Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AUP74_RS16665 Genome accession   NZ_CP014143
Coordinates   3805352..3807064 (+) Length   570 a.a.
NCBI ID   WP_069948547.1    Uniprot ID   A0A1C9WC80
Organism   Microbulbifer aggregans strain CCB-MM1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3800352..3812064
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AUP74_RS16650 (AUP74_03352) pilE 3802426..3802962 (-) 537 WP_269465979.1 pilin Machinery gene
  AUP74_RS16655 (AUP74_03353) tfpZ 3803445..3804245 (-) 801 WP_158514578.1 TfpX/TfpZ family type IV pilin accessory protein -
  AUP74_RS16660 (AUP74_03354) pilE 3804263..3804799 (-) 537 WP_069948983.1 pilin Machinery gene
  AUP74_RS16665 (AUP74_03355) pilB 3805352..3807064 (+) 1713 WP_069948547.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AUP74_RS16670 (AUP74_03356) pilC 3807173..3808384 (+) 1212 WP_069948548.1 type II secretion system F family protein Machinery gene
  AUP74_RS16675 (AUP74_03357) - 3808398..3809282 (+) 885 WP_069948549.1 prepilin peptidase -
  AUP74_RS16680 (AUP74_03358) coaE 3809366..3809977 (+) 612 WP_069948550.1 dephospho-CoA kinase -
  AUP74_RS16685 (AUP74_03359) yacG 3809984..3810208 (+) 225 WP_069948551.1 DNA gyrase inhibitor YacG -
  AUP74_RS16690 (AUP74_03360) mutT 3810378..3810782 (-) 405 WP_069948552.1 8-oxo-dGTP diphosphatase MutT -
  AUP74_RS17520 - 3810973..3811104 (-) 132 WP_257785519.1 hypothetical protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62327.17 Da        Isoelectric Point: 5.3382

>NTDB_id=168706 AUP74_RS16665 WP_069948547.1 3805352..3807064(+) (pilB) [Microbulbifer aggregans strain CCB-MM1]
MSSIPLSGLAKRLVADQKLDESTAVSATKAAQRENQTFAQHAVEAKLIKSRELASIAASAFGSPLFDLSGYNFDLMPKGL
VDDRLISKHFALPLYKRGNRLFVAVADPTNLAALDELNFNTGLNTDAVLVEADKLAKAIDSYLSNSNDMGAGLDGMDDED
LDALDVEGGEPDQAQDDNPGGDEAPVVRFVNKVLLDAIRSGASDIHFEPYEKAYRVRFRTDGVLHEIAKPPIQLAPRISA
RLKVMSKMDISERRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKLGIDALGYEESQKKMYLDTLAQP
QGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGINQVNVNPKVGLNFADALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIATSVSLIIAQRLARRLCNECKKPVNLPDEVLKEEGF
DSVTIPKNEWKIYQPVGCEHCSKGYKGRVGVYEVVRITDGISRIIMEGGNSIQIADQARKEGFNDLRISALRKVVMGITS
LEEANRVTND

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=168706 AUP74_RS16665 WP_069948547.1 3805352..3807064(+) (pilB) [Microbulbifer aggregans strain CCB-MM1]
ATGAGCAGTATTCCTCTAAGTGGCCTTGCCAAACGGCTGGTAGCAGACCAGAAACTGGATGAATCCACAGCTGTCTCCGC
CACCAAGGCGGCTCAGCGTGAAAACCAGACTTTTGCCCAACATGCGGTTGAAGCCAAACTGATCAAGAGCCGTGAGCTAG
CCAGTATTGCCGCCAGCGCCTTCGGCTCCCCTCTGTTCGACCTCAGTGGTTACAATTTCGACCTGATGCCCAAGGGGCTG
GTCGATGATCGCCTGATCAGCAAGCACTTTGCCCTGCCCTTGTACAAGCGTGGCAACCGACTGTTTGTAGCCGTAGCAGA
CCCTACCAACCTGGCCGCACTGGATGAACTCAACTTCAATACCGGCCTGAATACCGATGCGGTGCTGGTTGAAGCTGACA
AACTTGCCAAGGCAATCGACAGCTACCTCTCCAACAGCAATGACATGGGGGCAGGCCTTGATGGAATGGATGATGAGGAC
CTTGATGCCCTGGATGTAGAGGGTGGCGAGCCTGATCAAGCGCAAGATGATAACCCTGGCGGCGACGAAGCCCCGGTTGT
ACGTTTTGTAAACAAAGTACTCCTCGATGCCATCCGTTCAGGCGCTTCGGATATCCATTTCGAACCTTACGAGAAAGCCT
ATCGGGTCCGCTTCCGTACTGATGGTGTGCTGCACGAAATAGCAAAGCCGCCCATTCAGCTGGCCCCGCGTATCTCCGCG
CGCCTAAAGGTCATGTCCAAGATGGATATCTCCGAGCGGCGAGTCCCTCAGGATGGCCGTATCAAGATGAAGCTGTCCAA
GACCAAGGCAATCGACTTCCGGGTCAATAGCCTCCCGACCCTCTGGGGGGAAAAGATCGTACTGCGGATCCTTGACCCCT
CCTCTGCCAAACTGGGCATCGACGCGCTCGGCTATGAGGAAAGTCAGAAGAAGATGTACCTGGATACCCTTGCCCAGCCA
CAGGGCATGATTCTTGTGACAGGCCCTACCGGCTCGGGTAAGACGGTCTCCCTCTATACCGGCCTCAATATCCTCAATAC
CGCCGAACGGAACATCTCCACCGCGGAGGACCCTGTGGAGATCAACCTGGAGGGGATCAACCAGGTTAACGTTAACCCCA
AAGTGGGCCTTAACTTTGCAGATGCTCTACGCTCTTTCCTTCGCCAGGATCCCGATGTGGTGATGGTAGGTGAGATCCGT
GACCTGGAAACAGCCGAGATTGCCATCAAGGCAGCCCAGACGGGGCACTTAGTGCTATCAACCCTACACACCAACTCCGC
TCCAGAGACACTGACCCGCCTGATGAATATGGGTGTGCCCACATTCAACATCGCAACTTCGGTAAGCCTGATCATAGCCC
AGCGGTTGGCCCGTAGGCTCTGCAACGAGTGCAAAAAGCCGGTTAACCTGCCGGATGAAGTGCTGAAGGAAGAAGGGTTT
GACTCAGTCACTATTCCAAAGAACGAATGGAAGATATATCAACCCGTGGGCTGTGAGCACTGCTCTAAAGGCTACAAAGG
CCGGGTGGGCGTCTATGAAGTAGTTCGCATCACCGATGGCATCTCCAGAATTATCATGGAGGGGGGTAACTCCATACAGA
TTGCAGACCAAGCCCGCAAAGAGGGCTTTAATGATCTGCGCATATCAGCCCTCCGCAAGGTGGTGATGGGAATAACCAGC
CTAGAAGAGGCAAACAGGGTTACCAATGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C9WC80

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60

100

0.6
  pilB Acinetobacter baylyi ADP1

58.554

99.474

0.582
  pilB Legionella pneumophila strain ERS1305867

55.203

99.474

0.549
  pilB Vibrio parahaemolyticus RIMD 2210633

55.258

91.754

0.507
  pilB Vibrio cholerae strain A1552

54.064

92.807

0.502
  pilB Vibrio campbellii strain DS40M4

53.788

92.632

0.498
  pilF Neisseria gonorrhoeae MS11

49.387

100

0.495
  pilB/pilB1 Synechocystis sp. PCC 6803

42.2

87.719

0.37

Multiple sequence alignment