Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AL468_RS21495 Genome accession   NZ_CP014134
Coordinates   2540997..2542682 (-) Length   561 a.a.
NCBI ID   WP_104974818.1    Uniprot ID   -
Organism   Vibrio diabolicus strain FDAARGOS_99     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2535997..2547682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL468_RS21465 (AL468_12880) rplS 2536077..2536430 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  AL468_RS21470 (AL468_12885) yacG 2537091..2537285 (-) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  AL468_RS21475 (AL468_12890) zapD 2537433..2538173 (-) 741 WP_104974815.1 cell division protein ZapD -
  AL468_RS21480 (AL468_12895) coaE 2538201..2538815 (-) 615 WP_047008979.1 dephospho-CoA kinase -
  AL468_RS21485 (AL468_12900) pilD 2538816..2539685 (-) 870 WP_104974816.1 A24 family peptidase Machinery gene
  AL468_RS21490 (AL468_12905) pilC 2539750..2540973 (-) 1224 WP_104974817.1 type II secretion system F family protein Machinery gene
  AL468_RS21495 (AL468_12910) pilB 2540997..2542682 (-) 1686 WP_104974818.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AL468_RS21500 (AL468_12915) pilA 2542688..2543161 (-) 474 WP_012841419.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AL468_RS21510 (AL468_12925) nadC 2543424..2544311 (-) 888 WP_104974819.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AL468_RS21515 (AL468_12930) ampD 2544404..2544955 (+) 552 WP_104974820.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AL468_RS21520 (AL468_12935) pdhR 2545361..2546128 (+) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62229.04 Da        Isoelectric Point: 5.6670

>NTDB_id=168590 AL468_RS21495 WP_104974818.1 2540997..2542682(-) (pilB) [Vibrio diabolicus strain FDAARGOS_99]
MLTNLSTALRQKGLLTFSQEESLIEQVKASGISMPEALLSSGFFTSSELAEHLSSIFGLNQPELSQYEYASLCQQLGLRE
LITRHNALPLQRTPSTLLLAVADPTNQQTEDDFRFATGLQVELVLADFRQLSAAIRRLYGRSLSHEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNHDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKQSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=168590 AL468_RS21495 WP_104974818.1 2540997..2542682(-) (pilB) [Vibrio diabolicus strain FDAARGOS_99]
ATGTTGACCAACCTATCCACGGCTCTTCGCCAAAAAGGGTTACTGACCTTTTCCCAAGAAGAATCGTTAATAGAGCAAGT
CAAAGCTTCTGGCATTTCGATGCCAGAGGCTTTACTCAGTTCTGGATTCTTCACGTCAAGCGAACTGGCCGAGCACTTAA
GTTCTATTTTTGGTTTAAACCAGCCTGAGTTATCTCAATATGAATATGCTTCGCTTTGCCAACAGCTCGGCCTACGTGAA
TTAATCACACGACATAACGCACTCCCACTCCAGCGTACGCCTTCAACTTTGTTATTAGCCGTTGCCGACCCGACGAACCA
ACAAACAGAAGATGACTTTCGTTTTGCGACAGGGTTACAGGTTGAACTAGTCTTGGCGGATTTTCGTCAACTCAGCGCGG
CAATTCGACGCTTGTATGGTCGTTCATTGAGTCATGAGAAGTCTGGATTAAAAGAGATCAACCAAGAAGAGTTAGCCAAT
CTGGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATAAA
CCAAATTCTGTTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAACCTTATGAAAAGATGTACCGCGTTCGCC
TACGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTAAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCACGACACCGCGAT
TGATATGCGTGTATCCACGCTACCGACTCTATTCGGAGAGAAAATTGTATTACGACTGCTCGACAGCAGCTCTGCGTCAC
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGCCCAACTGGGAGCGGGAAAACCGTTTCTCTGTACACTGGGCTGAGTATTCTCAATAAACCAGAAATCAA
CATTTCCACGGCAGAAGACCCCGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCCCTGCGCTCTTTCTTACGTCAAGACCCCGATGTAGTAATGGTCGGTGAGATTCGTGATTTAGATACC
GCCGAAATCGCCATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTACATACCAATTCTGCAGCTGAAACGGT
GATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCATCTCTTAGTTTGATTATTGCCCAGCGCTTAGCCC
GTAAACTGTGCTCGCACTGCAAACAATCACAAGAGCTTACCGTTCAGCTACAACATCTCGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGCAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATAAAAGGCGCATCAGTGCATGAGTTAGAAAAACTCGCCATCGCAAACGGCA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGAATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

96.078

100

0.961
  pilB Vibrio campbellii strain DS40M4

90.018

100

0.9
  pilB Vibrio cholerae strain A1552

74.555

100

0.747
  pilB Acinetobacter baumannii D1279779

51.036

94.652

0.483
  pilB Legionella pneumophila strain ERS1305867

49.353

96.435

0.476
  pilB Acinetobacter baylyi ADP1

47.397

99.287

0.471
  pilF Neisseria gonorrhoeae MS11

44.858

100

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

37.056

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.332

100

0.367

Multiple sequence alignment