Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AL468_RS21500 Genome accession   NZ_CP014134
Coordinates   2542688..2543161 (-) Length   157 a.a.
NCBI ID   WP_012841419.1    Uniprot ID   -
Organism   Vibrio diabolicus strain FDAARGOS_99     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2537688..2548161
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL468_RS21480 (AL468_12895) coaE 2538201..2538815 (-) 615 WP_047008979.1 dephospho-CoA kinase -
  AL468_RS21485 (AL468_12900) pilD 2538816..2539685 (-) 870 WP_104974816.1 A24 family peptidase Machinery gene
  AL468_RS21490 (AL468_12905) pilC 2539750..2540973 (-) 1224 WP_104974817.1 type II secretion system F family protein Machinery gene
  AL468_RS21495 (AL468_12910) pilB 2540997..2542682 (-) 1686 WP_104974818.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AL468_RS21500 (AL468_12915) pilA 2542688..2543161 (-) 474 WP_012841419.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AL468_RS21510 (AL468_12925) nadC 2543424..2544311 (-) 888 WP_104974819.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AL468_RS21515 (AL468_12930) ampD 2544404..2544955 (+) 552 WP_104974820.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AL468_RS21520 (AL468_12935) pdhR 2545361..2546128 (+) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16575.17 Da        Isoelectric Point: 8.4643

>NTDB_id=168591 AL468_RS21500 WP_012841419.1 2542688..2543161(-) (pilA) [Vibrio diabolicus strain FDAARGOS_99]
MKNSKQKKQQGFTLIELMIVVAIIGILSAFAVPAYQNYTKKATLSEFPKAAAAAKLAVELCAHENASNETTFISECVKEE
NGIPAKFVLNNIEISVVKATDASVSGAIAVIAAADKVKGPIAKGEQYIMAASYKTQGLEWGASCKAGGKAQTDYCPN

Nucleotide


Download         Length: 474 bp        

>NTDB_id=168591 AL468_RS21500 WP_012841419.1 2542688..2543161(-) (pilA) [Vibrio diabolicus strain FDAARGOS_99]
ATGAAAAACAGTAAACAAAAAAAACAACAGGGTTTTACTCTGATTGAATTAATGATTGTGGTGGCGATTATTGGCATCTT
ATCTGCTTTCGCAGTTCCTGCTTACCAAAATTATACTAAGAAAGCGACATTGTCAGAATTTCCAAAAGCAGCTGCGGCAG
CCAAATTAGCAGTAGAATTATGTGCCCATGAGAATGCCTCAAATGAAACAACATTTATTAGTGAATGTGTAAAGGAAGAA
AATGGAATTCCCGCTAAATTCGTTTTGAATAATATTGAAATTAGCGTTGTTAAAGCCACAGATGCTAGCGTTTCTGGTGC
GATAGCAGTTATTGCAGCAGCTGATAAAGTTAAGGGACCTATTGCTAAAGGCGAGCAATATATCATGGCTGCATCATATA
AAACTCAAGGCTTAGAGTGGGGGGCTTCATGTAAAGCTGGCGGTAAAGCACAAACAGACTACTGCCCAAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

57.325

100

0.573
  pilA Haemophilus influenzae Rd KW20

43.056

91.72

0.395
  pilA Haemophilus influenzae 86-028NP

41.781

92.994

0.389

Multiple sequence alignment