Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AL542_RS07475 Genome accession   NZ_CP014056
Coordinates   828208..829902 (+) Length   564 a.a.
NCBI ID   WP_005502102.1    Uniprot ID   D0I4V9
Organism   Grimontia hollisae strain FDAARGOS_111     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 823208..834902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL542_RS07455 (AL542_07365) pdhR 824810..825583 (-) 774 WP_005502094.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AL542_RS07460 (AL542_07370) ampD 825891..826433 (-) 543 WP_005502096.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AL542_RS07465 (AL542_07375) nadC 826625..827530 (+) 906 WP_005502097.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AL542_RS07470 (AL542_07380) pilA 827758..828186 (+) 429 WP_040528180.1 pilin Machinery gene
  AL542_RS07475 (AL542_07385) pilB 828208..829902 (+) 1695 WP_005502102.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AL542_RS07480 (AL542_07390) pilC 829914..831137 (+) 1224 WP_005502104.1 type II secretion system F family protein Machinery gene
  AL542_RS07485 (AL542_07395) pilD 831245..832114 (+) 870 WP_005502106.1 prepilin peptidase Machinery gene
  AL542_RS07490 (AL542_07400) coaE 832118..832741 (+) 624 WP_005502108.1 dephospho-CoA kinase -
  AL542_RS07495 (AL542_07405) zapD 832731..833471 (+) 741 WP_005502110.1 cell division protein ZapD -
  AL542_RS07500 (AL542_07410) yacG 833506..833703 (+) 198 WP_040528217.1 DNA gyrase inhibitor YacG -
  AL542_RS07510 (AL542_07415) mutT 833934..834338 (-) 405 WP_005502113.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 61860.38 Da        Isoelectric Point: 5.6165

>NTDB_id=168122 AL542_RS07475 WP_005502102.1 828208..829902(+) (pilB) [Grimontia hollisae strain FDAARGOS_111]
MTSNLAVALSRAGYISPAQEQEVIAKVKAEGVSAPSALLALHILSSEALANTVSELFSEPLHSIKEYPWENTSQFLNQQD
LVLSSQALPLEIKHHQLFLGLSDPTRLDIQDDFQFATGYSIVPVLLEHAQIEGAIRRLYGKNVTERVKTSGVSDSELEAL
STNSDEITDTDDLFSDDAPVSRYIQQVLLDAVRKQASDIHFEPFEQRYQIRFRLDGILQTHSTPPVGISRRLSTRLKIIA
KLNIAERRLPQDGRIKLNLSENKSIDMRVSTLPTLWGEKVVLRILDGGSVALDINKLGYSQQQKDDYLAALDRPQGMILM
TGPTGSGKTVSLYTGLKYLNTAERNISTAEDPVEINLPGINQVNINNDIGLDFAKALRAFLRQDPDVVMVGEIRDLETAE
IGIKAAQTGHLVLSTLHTNSAAESVTRLSNMGVAPYNLAASLTLIIAQRLGRRLCQHCKVPHPPGDRHLTALTQHYPSLR
PEQVYQASPEGCDHCNNGYLGRVGIYEVMPITRELAEAIGNGASASQIETLAMGNGMLTLPLSGIEKLNEGITSLAELQR
VISL

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=168122 AL542_RS07475 WP_005502102.1 828208..829902(+) (pilB) [Grimontia hollisae strain FDAARGOS_111]
ATGACCAGCAACCTTGCTGTCGCCCTGAGCCGGGCTGGATATATTTCCCCGGCGCAGGAGCAAGAAGTGATAGCCAAAGT
CAAAGCCGAGGGTGTCAGCGCACCGTCGGCTTTGCTCGCTTTACATATTCTGAGCAGTGAAGCGCTGGCAAACACGGTAT
CCGAGCTTTTCAGTGAGCCACTGCACAGCATTAAAGAGTACCCCTGGGAGAACACCAGCCAGTTTCTCAACCAACAGGAT
CTGGTGCTCTCCAGCCAGGCCCTGCCGCTGGAGATTAAACACCACCAGCTGTTTCTGGGCCTCAGCGACCCTACCCGCCT
GGATATCCAGGATGATTTCCAGTTTGCCACCGGTTATTCGATTGTGCCGGTGCTGCTGGAACATGCCCAGATAGAAGGAG
CGATTCGCCGCCTGTACGGCAAAAATGTCACAGAACGGGTCAAAACCAGCGGCGTCTCGGATTCCGAACTGGAAGCGCTC
AGCACCAACAGTGATGAGATAACAGACACCGACGATCTGTTTTCCGATGATGCCCCGGTCAGCCGTTATATCCAGCAGGT
TCTTTTGGATGCGGTGCGCAAACAAGCCTCCGATATTCACTTTGAACCCTTTGAACAGCGCTACCAGATACGTTTCCGTC
TCGACGGCATTTTGCAAACTCACAGCACACCGCCGGTGGGGATCTCCCGCCGCCTTTCCACGCGCCTGAAAATCATCGCC
AAGCTCAATATTGCCGAGCGCCGGCTGCCACAGGATGGGCGAATCAAACTCAACCTGTCAGAAAACAAATCCATCGATAT
GCGGGTCTCTACCCTGCCCACCCTGTGGGGAGAAAAAGTGGTGCTGCGAATCCTCGATGGCGGCAGTGTGGCACTGGATA
TCAACAAACTGGGCTATAGCCAACAGCAAAAAGACGACTACCTTGCCGCCCTCGATCGCCCACAGGGGATGATCCTGATG
ACCGGGCCAACAGGCAGCGGGAAAACCGTCTCCTTGTATACCGGGCTGAAATACTTAAATACCGCCGAGCGCAATATCTC
TACCGCAGAAGACCCGGTAGAAATCAACCTGCCCGGTATCAACCAGGTCAATATCAACAACGATATCGGGCTCGATTTTG
CCAAGGCACTGCGGGCCTTCTTGCGTCAGGACCCGGATGTGGTGATGGTGGGTGAAATCCGGGATTTGGAAACCGCAGAA
ATCGGCATTAAAGCGGCGCAAACCGGCCATTTGGTGCTTTCCACTCTCCACACCAACTCTGCTGCTGAGAGCGTCACCCG
CCTGTCGAACATGGGGGTTGCCCCTTACAACCTGGCTGCGTCCCTGACCCTGATTATTGCCCAGCGTCTGGGACGCCGGC
TGTGCCAGCACTGTAAAGTGCCACATCCTCCCGGCGATCGCCACCTGACAGCGCTCACCCAGCATTATCCTTCGCTGAGG
CCGGAACAGGTTTACCAGGCATCGCCTGAAGGCTGTGACCATTGCAACAACGGCTATCTGGGACGGGTGGGAATTTATGA
GGTGATGCCCATCACCCGTGAGCTTGCCGAAGCCATTGGCAATGGCGCATCGGCATCACAGATTGAAACCCTCGCCATGG
GCAATGGCATGTTAACACTGCCACTTTCCGGCATTGAGAAACTAAATGAAGGCATCACCAGCCTTGCTGAACTGCAACGT
GTGATCAGCCTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(186-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D0I4V9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

58.584

100

0.587
  pilB Vibrio campbellii strain DS40M4

57.447

100

0.574
  pilB Vibrio parahaemolyticus RIMD 2210633

56.738

100

0.567
  pilB Acinetobacter baylyi ADP1

47.163

100

0.472
  pilB Legionella pneumophila strain ERS1305867

48.423

95.567

0.463
  pilB Acinetobacter baumannii D1279779

48.771

93.794

0.457
  pilF Neisseria gonorrhoeae MS11

43.173

96.099

0.415
  pilF Thermus thermophilus HB27

38.336

98.05

0.376

Multiple sequence alignment