Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AL542_RS07485 Genome accession   NZ_CP014056
Coordinates   831245..832114 (+) Length   289 a.a.
NCBI ID   WP_005502106.1    Uniprot ID   D0I4W1
Organism   Grimontia hollisae strain FDAARGOS_111     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 826245..837114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL542_RS07465 (AL542_07375) nadC 826625..827530 (+) 906 WP_005502097.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AL542_RS07470 (AL542_07380) pilA 827758..828186 (+) 429 WP_040528180.1 pilin Machinery gene
  AL542_RS07475 (AL542_07385) pilB 828208..829902 (+) 1695 WP_005502102.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AL542_RS07480 (AL542_07390) pilC 829914..831137 (+) 1224 WP_005502104.1 type II secretion system F family protein Machinery gene
  AL542_RS07485 (AL542_07395) pilD 831245..832114 (+) 870 WP_005502106.1 prepilin peptidase Machinery gene
  AL542_RS07490 (AL542_07400) coaE 832118..832741 (+) 624 WP_005502108.1 dephospho-CoA kinase -
  AL542_RS07495 (AL542_07405) zapD 832731..833471 (+) 741 WP_005502110.1 cell division protein ZapD -
  AL542_RS07500 (AL542_07410) yacG 833506..833703 (+) 198 WP_040528217.1 DNA gyrase inhibitor YacG -
  AL542_RS07510 (AL542_07415) mutT 833934..834338 (-) 405 WP_005502113.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 289 a.a.        Molecular weight: 31710.75 Da        Isoelectric Point: 6.2123

>NTDB_id=168124 AL542_RS07485 WP_005502106.1 831245..832114(+) (pilD) [Grimontia hollisae strain FDAARGOS_111]
MDWLFDSPGLYVFLASLFGLIVGSFLNVVIYRLPKMMESQWRQECHACFPQEMPAPSESEVFNLSLPRSHCPSCHAPVRA
IDNIPVLSWLILGGKCRSCKTNISVRYPLIEILTAVLSGVVAYTLSGYWSLAVIGATFVLVVLCFIDLDTLLLPDQMTLP
LMWAGMLLALTGLSPVSLQDAVIGAMAGYLSLWSVFQLFKLLTGKEGMGYGDFKLLAALGAWLGWQALPMVILMASMVGA
IGGIITMRVKGTGKETPFSFGPYLAIAGWISLLWGNMILDWYLTSYLGY

Nucleotide


Download         Length: 870 bp        

>NTDB_id=168124 AL542_RS07485 WP_005502106.1 831245..832114(+) (pilD) [Grimontia hollisae strain FDAARGOS_111]
ATGGATTGGCTTTTCGACTCCCCGGGTCTTTACGTTTTCCTTGCCTCACTGTTCGGCCTGATTGTAGGCAGCTTCCTCAA
TGTGGTTATCTATCGTTTACCCAAAATGATGGAGAGCCAGTGGCGACAGGAATGCCACGCCTGTTTTCCGCAAGAGATGC
CTGCGCCCAGTGAAAGTGAGGTATTCAACCTCAGCCTGCCGCGTTCTCACTGTCCGTCATGCCATGCGCCCGTCCGCGCG
ATTGACAACATTCCGGTATTAAGCTGGCTGATCCTTGGCGGCAAGTGCCGTTCCTGCAAAACCAACATCAGCGTCCGCTA
TCCCTTGATTGAAATTCTGACTGCCGTGCTGTCAGGTGTTGTGGCCTATACGCTGTCAGGGTATTGGTCGCTTGCCGTGA
TTGGTGCCACCTTTGTGCTGGTTGTGCTCTGTTTTATCGACCTTGATACCCTGTTGCTGCCAGACCAGATGACACTGCCC
CTGATGTGGGCCGGCATGTTACTTGCGCTTACCGGTCTCTCCCCGGTTTCACTCCAGGATGCGGTGATCGGCGCGATGGC
GGGATACCTGTCACTGTGGAGCGTGTTCCAGCTGTTTAAGCTGCTGACGGGCAAGGAAGGGATGGGTTATGGCGATTTCA
AGCTCCTTGCAGCCCTTGGGGCCTGGCTGGGTTGGCAGGCATTGCCTATGGTTATTCTGATGGCGTCTATGGTTGGTGCG
ATTGGTGGCATCATCACAATGCGCGTGAAAGGCACAGGGAAAGAAACACCTTTTTCATTTGGCCCTTACCTGGCCATTGC
GGGCTGGATAAGTTTGCTGTGGGGCAATATGATCCTGGACTGGTATTTAACATCTTATCTGGGTTATTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)

Type IV leader peptidase family (Peptidase_A24)

(138-243)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D0I4W1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

61.938

100

0.619
  pilD Vibrio campbellii strain DS40M4

63.475

97.578

0.619
  pilD Neisseria gonorrhoeae MS11

50.903

95.848

0.488
  pilD Acinetobacter nosocomialis M2

49.462

96.54

0.478
  pilD Acinetobacter baumannii D1279779

49.462

96.54

0.478

Multiple sequence alignment