Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AL541_RS12695 Genome accession   NZ_CP014040
Coordinates   2551321..2553006 (+) Length   561 a.a.
NCBI ID   WP_104979679.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain FDAARGOS_110     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2546321..2558006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL541_RS12675 (AL541_21045) pdhR 2547904..2548671 (-) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AL541_RS12680 (AL541_21050) ampD 2549077..2549628 (-) 552 WP_017635914.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AL541_RS12685 (AL541_21055) nadC 2549721..2550608 (+) 888 WP_017635915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AL541_RS12690 (AL541_21060) - 2550871..2551314 (+) 444 WP_064354289.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AL541_RS12695 (AL541_21065) pilB 2551321..2553006 (+) 1686 WP_104979679.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AL541_RS12700 (AL541_21070) pilC 2553030..2554253 (+) 1224 WP_017635918.1 type II secretion system F family protein Machinery gene
  AL541_RS12705 (AL541_21075) pilD 2554318..2555187 (+) 870 WP_017635919.1 A24 family peptidase Machinery gene
  AL541_RS12710 (AL541_21080) coaE 2555188..2555802 (+) 615 WP_017635920.1 dephospho-CoA kinase -
  AL541_RS12715 (AL541_21085) zapD 2555830..2556570 (+) 741 WP_005373086.1 cell division protein ZapD -
  AL541_RS12720 (AL541_21090) yacG 2556729..2556923 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  AL541_RS12725 (AL541_21095) rplS 2557578..2557931 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62276.10 Da        Isoelectric Point: 5.5338

>NTDB_id=167828 AL541_RS12695 WP_104979679.1 2551321..2553006(+) (pilB) [Vibrio alginolyticus strain FDAARGOS_110]
MLTNLSTILRQKGVLTFSQEESLIEQVKASGISMPEALLSSGFFTSSELADHLSSLFGLNQPELSQYEYASLCQQLGLRE
LITRHNALPLQRTSSTLLLAVADPTNQQAEDDFRFATGLQVELVLADFRQLSAAIRRLYGRSLNHEKSGLKEINQEELAN
LVDVGVDEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLNLIIAQRLARKLCSHCKQSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=167828 AL541_RS12695 WP_104979679.1 2551321..2553006(+) (pilB) [Vibrio alginolyticus strain FDAARGOS_110]
ATGTTAACAAACCTCTCAACCATTCTTCGTCAAAAAGGAGTACTGACCTTTTCCCAAGAAGAATCGTTAATAGAGCAAGT
CAAAGCTTCTGGCATTTCGATGCCGGAAGCTTTACTCAGTTCTGGATTCTTCACGTCAAGTGAGCTCGCTGATCACTTAA
GTTCTCTCTTTGGTTTAAATCAGCCCGAGTTATCTCAGTACGAGTACGCTTCGCTTTGTCAACAACTCGGTCTACGTGAA
TTAATCACACGACATAACGCACTCCCACTCCAGCGTACGTCTTCAACGTTATTATTAGCGGTTGCCGACCCCACCAACCA
ACAAGCCGAAGATGACTTCCGCTTTGCCACTGGGTTACAGGTTGAACTAGTCTTGGCGGATTTTCGTCAACTCAGCGCGG
CAATTCGACGCTTGTATGGTCGTTCATTGAATCATGAGAAGTCTGGATTAAAAGAGATCAACCAAGAAGAATTAGCCAAC
CTGGTTGATGTGGGCGTAGATGAAATCGACAACATTGAAGACTTGAGCCAAGACGAGTCGCCCGTCAGCCGTTACATAAA
CCAAATTCTGCTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAGCCTTATGAAAAGATGTACCGTGTGCGCC
TACGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTAAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAACTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCAAT
TGATATGCGTGTATCCACGCTACCGACTCTATTCGGAGAGAAAATTGTACTACGACTGCTCGACAGCAGCTCTGCGTCAT
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGCCCAACCGGGAGCGGAAAAACCGTTTCTCTGTACACTGGGCTGAGTATCCTCAATAAGCCAGAAATCAA
CATTTCCACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCCCTGCGCTCTTTCTTACGTCAAGACCCGGATGTGGTAATGGTCGGTGAGATTCGTGACTTAGACACC
GCCGAAATCGCTATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTACATACCAATTCTGCGGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCATCTCTTAATTTGATTATTGCCCAGCGCTTGGCCC
GTAAACTGTGCTCGCACTGTAAACAATCACAAGAGCTTACCGTTCAGCTACAACATCTTGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGTAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGAATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGCGCCTCAGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

95.365

100

0.954
  pilB Vibrio campbellii strain DS40M4

90.196

100

0.902
  pilB Vibrio cholerae strain A1552

74.199

100

0.743
  pilB Acinetobacter baumannii D1279779

50.94

94.831

0.483
  pilB Legionella pneumophila strain ERS1305867

49.259

96.257

0.474
  pilB Acinetobacter baylyi ADP1

50.29

92.157

0.463
  pilF Neisseria gonorrhoeae MS11

44.858

100

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

37.182

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.508

100

0.369
  pilF Thermus thermophilus HB27

36.121

100

0.362

Multiple sequence alignment