Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AL537_RS01435 Genome accession   NZ_CP014036
Coordinates   310915..312600 (+) Length   561 a.a.
NCBI ID   WP_104970940.1    Uniprot ID   -
Organism   Vibrio diabolicus strain FDAARGOS_105     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 305915..317600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL537_RS01415 (AL537_01415) pdhR 307556..308323 (-) 768 WP_005396680.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AL537_RS01420 (AL537_01420) ampD 308729..309280 (-) 552 WP_104970938.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AL537_RS01425 (AL537_01425) nadC 309373..310260 (+) 888 WP_053809204.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AL537_RS01430 (AL537_01430) pilA 310522..310914 (+) 393 WP_104970939.1 pilin Machinery gene
  AL537_RS01435 (AL537_01435) pilB 310915..312600 (+) 1686 WP_104970940.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AL537_RS01440 (AL537_01440) pilC 312624..313847 (+) 1224 WP_005396687.1 type II secretion system F family protein Machinery gene
  AL537_RS01445 (AL537_01445) pilD 313912..314781 (+) 870 WP_012841418.1 A24 family peptidase Machinery gene
  AL537_RS01450 (AL537_01450) coaE 314782..315396 (+) 615 WP_047008979.1 dephospho-CoA kinase -
  AL537_RS01455 (AL537_01455) zapD 315424..316164 (+) 741 WP_005396691.1 cell division protein ZapD -
  AL537_RS01460 (AL537_01460) yacG 316323..316517 (+) 195 WP_005396692.1 DNA gyrase inhibitor YacG -
  AL537_RS01465 (AL537_01465) rplS 317001..317354 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62228.05 Da        Isoelectric Point: 5.6670

>NTDB_id=167721 AL537_RS01435 WP_104970940.1 310915..312600(+) (pilB) [Vibrio diabolicus strain FDAARGOS_105]
MLTNLSTALRQKGLLTFSQEESLIEQVKASGISMPEALLSSGLFTSSELAEHLSSLFSLSQPELSQYEYASLCQQLGLRE
LITRHNALPLQRTPSTLLLAVADPTNQQTEDDFRFATGLQVELVLADFRQLSTAIRRLYGRSLSHEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNHDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKQSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=167721 AL537_RS01435 WP_104970940.1 310915..312600(+) (pilB) [Vibrio diabolicus strain FDAARGOS_105]
ATGCTCACCAACCTATCCACGGCTCTTCGCCAAAAAGGGTTACTGACCTTTTCCCAAGAAGAATCGTTAATAGAGCAAGT
CAAAGCTTCTGGCATTTCGATGCCAGAGGCTTTACTCAGTTCTGGATTATTTACATCAAGCGAACTCGCTGAGCACTTAA
GTTCTCTCTTTAGTTTGAGTCAGCCCGAGTTATCTCAGTATGAGTACGCTTCGCTTTGTCAACAACTCGGTCTACGTGAA
TTAATCACTCGACATAACGCACTCCCACTCCAGCGTACGCCTTCAACTTTGTTATTAGCCGTTGCCGACCCGACGAACCA
ACAAACAGAAGATGACTTTCGTTTTGCGACAGGGTTACAGGTTGAACTAGTCTTGGCGGATTTTCGTCAACTCAGCACGG
CAATTCGACGCTTATATGGTCGTTCATTGAGTCATGAGAAGTCTGGATTAAAAGAGATCAACCAAGAAGAGTTAGCCAAT
CTGGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATAAA
CCAAATTCTGTTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAACCTTATGAAAAGATGTACCGCGTTCGCC
TACGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTAAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCATGACACCGCGAT
TGATATGCGTGTATCCACGCTACCGACTCTATTCGGAGAGAAAATTGTATTACGACTGCTCGACAGCAGCTCTGCGTCAC
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGCCCAACTGGGAGCGGGAAAACCGTTTCTCTGTACACTGGGCTGAGTATTCTCAATAAACCAGAAATCAA
CATTTCCACGGCAGAAGACCCGGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCCCTGCGCTCTTTCTTACGTCAAGACCCCGATGTAGTAATGGTCGGTGAGATTCGTGATTTAGATACC
GCCGAAATCGCCATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTACATACCAATTCTGCAGCTGAAACGGT
GATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCATCTCTTAGTTTGATTATTGCCCAGCGCTTAGCCC
GTAAACTGTGCTCGCACTGCAAACAATCACAAGAGCTTACCGTTCAGCTACAACATCTCGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGCAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGTGCCTCGGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

95.9

100

0.959
  pilB Vibrio campbellii strain DS40M4

89.661

100

0.897
  pilB Vibrio cholerae strain A1552

74.021

100

0.742
  pilB Acinetobacter baumannii D1279779

51.429

93.583

0.481
  pilB Acinetobacter baylyi ADP1

47.67

99.465

0.474
  pilB Legionella pneumophila strain ERS1305867

48.983

96.435

0.472
  pilF Neisseria gonorrhoeae MS11

45.213

100

0.455
  pilB/pilB1 Synechocystis sp. PCC 6803

37.225

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.155

100

0.365

Multiple sequence alignment