Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AL475_RS16695 Genome accession   NZ_CP014033
Coordinates   1830297..1832033 (+) Length   578 a.a.
NCBI ID   WP_104966439.1    Uniprot ID   -
Organism   Vibrio fluvialis strain FDAARGOS_100     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1825297..1837033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL475_RS16675 (AL475_08715) pilM 1827603..1828607 (+) 1005 WP_104966436.1 type IV pilus assembly protein PilM Machinery gene
  AL475_RS16680 (AL475_08720) pilN 1828597..1829187 (+) 591 WP_044362423.1 PilN domain-containing protein Machinery gene
  AL475_RS16685 (AL475_08725) pilO 1829180..1829770 (+) 591 WP_104966437.1 type 4a pilus biogenesis protein PilO Machinery gene
  AL475_RS16690 (AL475_08730) pilP 1829760..1830272 (+) 513 WP_104966438.1 pilus assembly protein PilP Machinery gene
  AL475_RS16695 (AL475_08735) pilQ 1830297..1832033 (+) 1737 WP_104966439.1 type IV pilus secretin PilQ Machinery gene
  AL475_RS16700 (AL475_08740) aroK 1832221..1832739 (+) 519 WP_004728569.1 shikimate kinase AroK -
  AL475_RS16705 (AL475_08745) aroB 1832767..1833852 (+) 1086 WP_044362429.1 3-dehydroquinate synthase -
  AL475_RS16710 (AL475_08750) - 1833898..1835406 (+) 1509 WP_104966440.1 AAA family ATPase -
  AL475_RS16715 (AL475_08755) - 1835501..1836331 (+) 831 WP_020329450.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62413.35 Da        Isoelectric Point: 4.5347

>NTDB_id=167661 AL475_RS16695 WP_104966439.1 1830297..1832033(+) (pilQ) [Vibrio fluvialis strain FDAARGOS_100]
MIKGLNASTAWGIRCLFVGVCLLLSAATLAQETAANQLKNIDFRINKDKAAVLIVELASASAVVDVQKTEQGLNIELLKT
DVSDDKLYLLDVRDFATAVENIEVFRQQPSTLLVATVQGEFNYDYSLKGNYLEVVIRKLKAEEKAKPKSVLEKEGKLISI
NFQDIPVRNVLQLIADYNEFNLVVSDSVRGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQLE
KARMAEELGDLQSDILIINFAKASDIAQMIGGDGAVSMLSERGSISIDERTNALLIRELPENIAVIRDIVASLDIPVKQV
QIEARIVTVNEGNLDELGVRWGFSSTNGSTSTGGSIESNLGAIGLYDGGNNSGGSGDGVAIDDFLNVNLAATSANASSIA
FQVAKLGADTLLDLELSAMQRESKADIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPD
NRLVLDLSVTQDRPGEVVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPVLGALFRRSYE
QMGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=167661 AL475_RS16695 WP_104966439.1 1830297..1832033(+) (pilQ) [Vibrio fluvialis strain FDAARGOS_100]
ATGATTAAAGGACTAAATGCCTCCACGGCGTGGGGAATACGTTGTCTGTTTGTGGGCGTATGCCTGCTGTTGTCTGCCGC
CACACTTGCCCAGGAAACGGCGGCCAATCAGCTGAAAAATATCGATTTTCGCATCAATAAAGATAAAGCGGCGGTATTGA
TTGTCGAACTGGCTTCCGCCTCGGCCGTGGTGGATGTGCAGAAAACCGAGCAAGGCCTGAATATCGAACTGCTGAAAACC
GATGTCAGCGACGACAAACTCTACCTGCTCGATGTGCGCGACTTTGCCACCGCAGTGGAAAACATCGAAGTGTTCCGCCA
GCAGCCCAGCACCTTGTTGGTCGCAACGGTGCAGGGGGAGTTCAATTATGATTACAGCCTGAAGGGTAACTATCTGGAAG
TGGTGATCCGCAAGCTAAAAGCCGAGGAGAAAGCCAAACCCAAAAGCGTATTAGAAAAAGAAGGCAAGCTGATTTCGATT
AACTTTCAGGATATTCCGGTGCGCAATGTTCTGCAGTTGATCGCAGACTACAACGAGTTCAATTTGGTGGTTTCCGATTC
AGTGCGCGGTAATCTGACGCTGCGCCTTGATGGCGTGCCCTGGCAGCAGGTGCTGGACATTATTCTGCAAGTCAAAGGTC
TGGATAAACGCGTCGATGGCAATGTGATTCTGGTGGCACCCAAAGAAGAGCTGGATCTGCGTGAAAAGCAGCAGTTGGAA
AAAGCCCGCATGGCGGAAGAACTCGGCGATCTGCAATCGGATATTCTCATCATCAACTTTGCCAAAGCGTCTGACATTGC
GCAGATGATTGGCGGCGATGGGGCGGTCAGTATGTTGTCGGAGCGTGGTTCGATCAGCATTGACGAACGCACCAACGCGC
TGTTGATTCGTGAACTGCCGGAGAACATTGCTGTGATTCGCGATATCGTGGCGTCGCTCGATATTCCGGTCAAGCAAGTA
CAGATTGAAGCACGTATTGTGACGGTCAATGAAGGCAACTTGGATGAACTGGGTGTGCGCTGGGGTTTCAGCTCCACCAA
TGGCAGCACGAGCACGGGTGGCTCGATTGAAAGTAATCTGGGGGCGATTGGTTTATACGATGGCGGTAATAATAGTGGCG
GTTCTGGTGATGGTGTTGCGATTGATGATTTCCTCAATGTTAACCTCGCAGCCACCTCAGCCAATGCGTCCAGCATTGCG
TTTCAGGTCGCCAAGCTCGGCGCCGATACCTTGCTCGATCTTGAACTGTCAGCGATGCAACGCGAGTCGAAAGCGGACAT
CATCTCCAGTCCGAGGCTGATCACTACCAACAAGCAGCCGGCGTATATTGAGCAGGGTACTGAGATTCCGTACCTGGAAT
CGTCATCCAGTGGCGCTACCAGTGTGGCATTCAAGAAGGCAGTACTCAGCCTCAAAGTGACGCCGCAAATCACCCCCGAT
AACCGTCTGGTGCTGGATTTGAGCGTCACTCAGGATCGGCCGGGTGAAGTGGTGAAGACTGGTACTGGCGAGGCGGTGGC
GATCAACACGCAGCGCATTGGTACACAGGTGCTGGTCAACAACGGTGAAACCGTGGTTCTGGGCGGCATTTTCCAGCACA
GCATTACGAATTCAGTCGACAAGGTGCCGCTGCTGGGTGACTTGCCCGTGTTGGGCGCGCTGTTCCGTCGCAGCTACGAA
CAAATGGGCAAAAGTGAGCTGCTGATCTTCGTAACACCGAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(259-319)

Bacterial type II and III secretion system protein (Secretin)

(418-576)

Secretin and TonB N terminus short domain (STN)

(180-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

81.52

100

0.817
  pilQ Vibrio cholerae strain A1552

81.52

100

0.817
  pilQ Vibrio campbellii strain DS40M4

79.053

94.983

0.751
  pilQ Legionella pneumophila strain Lp02

42.364

95.156

0.403
  pilQ Legionella pneumophila strain ERS1305867

42.364

95.156

0.403
  pilQ Pseudomonas aeruginosa PAK

40.721

96.021

0.391
  comQ Acinetobacter baylyi ADP1

37.629

100

0.379

Multiple sequence alignment