Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AL527_RS20305 Genome accession   NZ_CP014025
Coordinates   4487316..4488581 (-) Length   421 a.a.
NCBI ID   WP_050587290.1    Uniprot ID   A0A7S9L776
Organism   Pseudomonas fulva strain FDAARGOS_167     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4482316..4493581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL527_RS20290 (AL527_20290) - 4483988..4485580 (-) 1593 WP_027913512.1 SPOR domain-containing protein -
  AL527_RS20295 (AL527_20295) aroB 4485596..4486693 (-) 1098 WP_027913511.1 3-dehydroquinate synthase -
  AL527_RS20300 (AL527_20300) aroK 4486793..4487311 (-) 519 WP_027913510.1 shikimate kinase AroK -
  AL527_RS20305 (AL527_20305) pilQ 4487316..4488581 (-) 1266 WP_050587290.1 type IV pilus secretin PilQ Machinery gene
  AL527_RS20310 (AL527_20310) - 4488505..4489521 (-) 1017 WP_104926601.1 pilus assembly protein PilP -
  AL527_RS20315 (AL527_20315) - 4489518..4490054 (-) 537 WP_104926602.1 PilN domain-containing protein -
  AL527_RS20320 (AL527_20320) pilM 4490051..4490932 (-) 882 WP_027913507.1 type IV pilus biogenesis protein PilM -
  AL527_RS20325 (AL527_20325) - 4491108..4493558 (+) 2451 WP_027913506.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46052.61 Da        Isoelectric Point: 5.0888

>NTDB_id=167461 AL527_RS20305 WP_050587290.1 4487316..4488581(-) (pilQ) [Pseudomonas fulva strain FDAARGOS_167]
MSKTWKWLLATLLLPLTAMAAPYQGERLSLNFQDVEVRAVLQVLADHAGINLVASDDVQGSVTLRLEDVPWDQAIDLVLR
SKGLGRRHEGNVLLIAPLSTLSAQSFEGTSNPLDTQSQPMRRELMRIHYAKAADLSELLLASMSEDGILAGRGSLGVDER
TNTLVVHQPANRLAEIRQLIAQLDVPVRQVAIEARIVEANVDYQKGLGVRWGGDLYSAAPRAGKDLFVDLGVERGGSSLG
VGLLRGDVLLDLELSAMEKSGEGEIISQPKVVTADKETARILKGTEVPYQQSNKNGATTVSFREASLSLEVTPQITPDDS
VVMTVRVTKDEPDYVNALNDVPPIRKNEVNAKVRVENGQTIVIGGVYSTTQNNVVDKVPFFGDLPYVGRLFRRDVLQEKK
SELLVFLTPRIMSDQTIAVSR

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=167461 AL527_RS20305 WP_050587290.1 4487316..4488581(-) (pilQ) [Pseudomonas fulva strain FDAARGOS_167]
ATGAGCAAGACATGGAAATGGCTATTGGCAACCCTGCTGCTGCCATTGACGGCAATGGCGGCCCCTTACCAGGGTGAGCG
GCTGTCACTGAATTTTCAGGATGTCGAGGTACGCGCCGTGCTGCAGGTGCTTGCCGATCATGCCGGCATCAACCTGGTCG
CCAGCGACGACGTACAAGGGAGCGTCACCTTACGATTGGAAGATGTGCCTTGGGATCAAGCGATCGACCTGGTATTGCGC
AGCAAGGGCCTGGGTCGACGCCACGAAGGCAACGTGCTGCTGATCGCACCGCTTTCTACCCTGAGCGCGCAGTCGTTCGA
GGGCACCTCGAACCCACTCGACACGCAATCACAACCCATGCGTCGGGAACTGATGCGTATCCACTACGCCAAGGCCGCCG
ATTTGTCCGAGCTGTTACTGGCGAGCATGTCTGAGGACGGCATTCTTGCCGGCAGGGGCAGCCTGGGCGTGGATGAGCGC
ACCAACACGCTGGTGGTCCACCAGCCGGCGAATCGACTCGCCGAGATTCGGCAACTGATCGCCCAATTGGATGTGCCCGT
GCGTCAGGTAGCGATCGAGGCCCGTATCGTGGAGGCCAACGTCGACTACCAGAAAGGCCTGGGGGTGAGATGGGGCGGCG
ACCTGTACAGCGCCGCACCGAGAGCGGGCAAGGACCTGTTCGTTGACCTGGGCGTCGAGCGGGGCGGCAGCAGCCTTGGC
GTTGGTCTGTTGCGCGGCGACGTGCTCCTTGACCTTGAGCTCAGTGCCATGGAAAAGAGCGGTGAGGGCGAGATCATCTC
CCAGCCCAAGGTAGTGACCGCCGACAAGGAGACGGCGCGGATACTCAAAGGTACCGAGGTGCCTTACCAGCAGTCCAACA
AGAATGGCGCGACAACCGTGTCGTTTCGCGAAGCCTCGCTTTCACTGGAGGTGACGCCGCAGATCACGCCGGATGACAGT
GTCGTCATGACCGTGAGAGTGACCAAGGACGAACCTGACTACGTCAATGCCCTCAATGACGTCCCGCCCATTCGCAAGAA
CGAGGTCAATGCCAAGGTCAGGGTCGAGAACGGGCAGACTATCGTGATCGGTGGCGTCTACTCCACCACGCAAAACAATG
TAGTGGACAAGGTGCCATTTTTTGGCGATCTGCCGTATGTTGGACGGCTGTTTCGACGCGATGTACTACAAGAGAAAAAA
TCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGACGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(125-189)

Bacterial type II and III secretion system protein (Secretin)

(256-412)

Secretin and TonB N terminus short domain (STN)

(51-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S9L776

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

54.773

100

0.572
  pilQ Acinetobacter baumannii strain A118

41.048

100

0.447
  pilQ Acinetobacter baumannii D1279779

41.048

100

0.447
  comQ Acinetobacter baylyi ADP1

41.685

100

0.447
  pilQ Legionella pneumophila strain ERS1305867

43.062

99.287

0.428
  pilQ Legionella pneumophila strain Lp02

42.584

99.287

0.423
  pilQ Vibrio campbellii strain DS40M4

39.906

100

0.404
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.625

98.812

0.401
  pilQ Vibrio cholerae strain A1552

40.625

98.812

0.401

Multiple sequence alignment