Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   RD2015_RS04955 Genome accession   NZ_CP013729
Coordinates   1129010..1130242 (-) Length   410 a.a.
NCBI ID   WP_058933954.1    Uniprot ID   A0A0U3CVY3
Organism   Roseateles depolymerans strain KCTC 42856     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1124010..1135242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RD2015_RS04930 (RD2015_995) - 1125520..1126143 (+) 624 WP_058933950.1 HAD family hydrolase -
  RD2015_RS04935 - 1126285..1126509 (-) 225 WP_058933951.1 DNA gyrase inhibitor YacG -
  RD2015_RS04940 (RD2015_997) zapD 1126625..1127380 (-) 756 WP_058933952.1 cell division protein ZapD -
  RD2015_RS04945 (RD2015_998) coaE 1127458..1128051 (-) 594 WP_198164897.1 dephospho-CoA kinase -
  RD2015_RS04950 (RD2015_999) - 1128068..1129006 (-) 939 WP_058937209.1 prepilin peptidase -
  RD2015_RS04955 (RD2015_1000) pilC 1129010..1130242 (-) 1233 WP_058933954.1 type II secretion system F family protein Machinery gene
  RD2015_RS04960 (RD2015_1001) pilF 1130341..1132065 (-) 1725 WP_058933955.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RD2015_RS04965 (RD2015_1002) - 1132500..1132874 (+) 375 WP_083525346.1 glutaredoxin family protein -
  RD2015_RS04970 (RD2015_1003) - 1132989..1134656 (-) 1668 WP_058933957.1 hypothetical protein -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 44949.71 Da        Isoelectric Point: 10.0311

>NTDB_id=164526 RD2015_RS04955 WP_058933954.1 1129010..1130242(-) (pilC) [Roseateles depolymerans strain KCTC 42856]
MATTTAGSRAKQGIKEYIFEWEGKDRNGKLVRGEMRAGGEAVVSASLRRQGILVSKVKKRRSGGGRSIKQKDISIFTRQL
ATMMRAGVPLLQAFDIVARGSTNPRLTKLLNEIRQDVETGTSLSVAFRKHPLYFDALYCNLVEAGESGGILEALLDRLAI
YQEKTVALKQKIKSALTYPIAVLVAAFVVTAIIMIFVVPTFRDVFKSFGADLPAPTLFVIWLSDIFVNYWWLIFAAVGGG
LYFFFQSWKRSVRMQKVMDRLLLKVPIFGDLIYKSVIARWTRTLATMFAAGVPLVEALDSVGGASGNAVFVEGTERIQRD
VSTGASLTNSMQTTGLFPVMVLQMSSIGEESGSLDHMLGKAADFYEEEVDDAVKSLSSLMEPIIIVFLGTLIGGIVVAMY
LPIFKLGAVV

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=164526 RD2015_RS04955 WP_058933954.1 1129010..1130242(-) (pilC) [Roseateles depolymerans strain KCTC 42856]
ATGGCGACGACCACTGCGGGTTCCCGCGCAAAGCAGGGCATCAAGGAATACATCTTTGAGTGGGAAGGCAAGGACCGCAA
CGGCAAGCTGGTGCGCGGTGAAATGCGCGCCGGTGGTGAAGCCGTGGTCAGCGCCTCGCTGCGCCGCCAGGGCATCCTGG
TCAGCAAGGTCAAGAAGCGTCGCTCCGGTGGCGGCCGCTCGATCAAGCAGAAGGACATCTCCATCTTCACCCGCCAGCTG
GCGACCATGATGCGCGCGGGTGTGCCGCTGCTGCAGGCCTTCGACATCGTGGCCCGCGGCAGCACCAACCCCCGCCTGAC
CAAACTGCTCAACGAGATCCGCCAGGACGTGGAGACCGGCACCAGCCTGTCGGTGGCCTTCCGCAAGCACCCGCTCTACT
TCGACGCGCTGTATTGCAACCTGGTGGAAGCCGGTGAGTCCGGCGGTATCCTGGAAGCGCTGCTGGACCGTCTGGCCATC
TACCAGGAAAAGACGGTGGCCCTGAAGCAGAAGATCAAGTCGGCACTCACCTACCCGATCGCCGTGCTGGTGGCCGCGTT
CGTGGTGACGGCCATCATCATGATCTTCGTGGTGCCGACCTTCCGCGACGTGTTCAAGAGCTTCGGTGCGGACCTGCCGG
CCCCGACCCTGTTCGTGATCTGGCTGTCCGACATCTTCGTCAACTACTGGTGGCTGATCTTCGCCGCGGTCGGCGGCGGT
CTCTACTTCTTCTTCCAGAGCTGGAAGCGCTCGGTCCGCATGCAGAAGGTCATGGACCGCCTGCTGCTGAAGGTGCCCAT
CTTTGGCGACCTGATCTACAAGTCGGTCATCGCTCGCTGGACCCGCACCCTGGCCACCATGTTCGCCGCAGGCGTGCCGC
TGGTCGAAGCGTTGGACTCGGTGGGCGGTGCCTCCGGCAATGCGGTCTTCGTCGAAGGCACGGAGCGCATCCAGCGCGAT
GTGTCCACCGGCGCATCGCTGACCAATTCGATGCAGACCACCGGCCTGTTCCCGGTCATGGTGCTGCAGATGTCGTCCAT
CGGCGAAGAGTCCGGCTCGCTGGACCACATGCTGGGCAAGGCGGCTGACTTCTACGAAGAAGAAGTGGACGATGCGGTGA
AGTCGCTGTCCAGCCTGATGGAGCCGATCATCATCGTCTTCCTGGGTACGCTGATTGGCGGCATCGTGGTGGCCATGTAC
CTGCCCATCTTCAAGCTCGGCGCGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(76-199)

Type II secretion system (T2SS), protein F (T2SSF)

(280-402)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0U3CVY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.455

98.537

0.537
  pilG Neisseria meningitidis 44/76-A

53.904

96.829

0.522
  pilG Neisseria gonorrhoeae MS11

53.904

96.829

0.522
  pilC Legionella pneumophila strain ERS1305867

51.768

96.585

0.5
  pilC Acinetobacter baylyi ADP1

49.749

97.073

0.483
  pilC Acinetobacter baumannii D1279779

49.874

96.829

0.483
  pilC Vibrio cholerae strain A1552

41.872

99.024

0.415
  pilC Vibrio campbellii strain DS40M4

40.299

98.049

0.395
  pilC Thermus thermophilus HB27

39.348

97.317

0.383

Multiple sequence alignment