Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AT705_RS09730 Genome accession   NZ_CP013611
Coordinates   2201016..2202692 (+) Length   558 a.a.
NCBI ID   WP_058796440.1    Uniprot ID   A0A0U3IIT1
Organism   Pseudoalteromonas rubra strain SCSIO 6842     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2196016..2207692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AT705_RS09705 (AT705_09705) nadC 2196407..2197246 (+) 840 WP_058796435.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AT705_RS26010 (AT705_09710) - 2197644..2198108 (+) 465 WP_058796436.1 pilin -
  AT705_RS09715 (AT705_09715) pilC 2198162..2199394 (+) 1233 WP_058796437.1 type II secretion system F family protein Machinery gene
  AT705_RS09720 (AT705_09720) pilD 2199417..2200292 (+) 876 WP_058796438.1 prepilin peptidase Machinery gene
  AT705_RS09725 (AT705_09725) coaE 2200296..2200907 (+) 612 WP_058796439.1 dephospho-CoA kinase -
  AT705_RS09730 (AT705_09730) pilB 2201016..2202692 (+) 1677 WP_058796440.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AT705_RS09735 (AT705_09735) yacG 2202764..2203006 (+) 243 WP_058796441.1 DNA gyrase inhibitor YacG -
  AT705_RS25485 (AT705_09740) - 2203270..2203779 (-) 510 WP_058796442.1 Spy/CpxP family protein refolding chaperone -
  AT705_RS09745 (AT705_09745) - 2203987..2204673 (+) 687 WP_058796443.1 response regulator transcription factor -
  AT705_RS09750 (AT705_09750) - 2204675..2206054 (+) 1380 WP_058796444.1 ATP-binding protein -
  AT705_RS09755 (AT705_09755) - 2206044..2207024 (+) 981 WP_058796445.1 alpha/beta fold hydrolase -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62200.28 Da        Isoelectric Point: 5.5614

>NTDB_id=163213 AT705_RS09730 WP_058796440.1 2201016..2202692(+) (pilB) [Pseudoalteromonas rubra strain SCSIO 6842]
MEHHSPLLRKFITLGKLTAEQVKSKQADAQSTAELICLCSGMSSQEMAEQCLDLFRVPYFDLSGFDINQVPKELVKEKLI
RQHHLLPLVKKDRKLFIATSDPTDYGAFENFEFSTGLQCEVVVVDYKLLDQKIDQLFDDNSSLTLSEDEFKEFASMDVED
EPKAQNNDEEKDDAPIIVYINKILMDAIKKGASDLHFEPYEKKYRVRFRIDGLLHEMASPPHALSSRLSARIKVMSHLDI
AEKRKPQDGRIKLKISERKSIDFRVSTLPTMWGEKIVMRILDSSAAKLGIEALGYEADQKSMYLNALDQPQGMILVTGPT
GSGKTVSLYTGLNILNKPERNISTAEDPVEINLEGINQVQINTKADMTFANALRAFLRQDPDVVMVGEIRDLETAEIAIK
AAQTGHLVMSTLHTNSAAETLTRLLNMGVPAYNVASSVSLIIAQRLARRLCPKCKEPEQLPEEELQRQGFSAEQIANMTL
YAPKGCDHCTDGYKGRVGIYEVMKITPEISHAIMEGGNSLEIAELAASKGFANLRQSGLKKAADGITSLAEVNRVTNY

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=163213 AT705_RS09730 WP_058796440.1 2201016..2202692(+) (pilB) [Pseudoalteromonas rubra strain SCSIO 6842]
ATGGAACATCACTCGCCGTTACTGAGAAAGTTTATTACCCTTGGCAAACTGACTGCCGAACAGGTTAAATCCAAACAAGC
CGATGCACAGTCAACCGCTGAGCTCATTTGCCTATGTAGTGGTATGAGCAGCCAGGAGATGGCCGAGCAATGCCTTGACT
TGTTCAGGGTACCCTATTTTGATCTGAGCGGCTTTGACATTAATCAGGTCCCTAAGGAACTGGTCAAAGAAAAGCTCATT
CGCCAGCACCATTTGCTGCCGCTGGTGAAAAAGGATCGCAAACTCTTTATTGCGACCTCAGATCCTACCGATTACGGGGC
TTTCGAGAATTTTGAATTCAGTACCGGTTTGCAATGTGAAGTCGTCGTTGTTGACTATAAACTGCTCGACCAGAAAATTG
ACCAACTGTTTGACGATAACAGCAGCCTAACCCTGAGTGAGGATGAATTTAAAGAGTTCGCCAGCATGGATGTTGAGGAC
GAACCCAAAGCACAAAACAACGATGAAGAAAAAGACGATGCCCCTATCATCGTTTATATCAATAAGATCCTCATGGATGC
GATCAAAAAAGGCGCTTCGGATCTGCACTTTGAGCCCTATGAAAAGAAATACCGGGTGCGCTTTCGTATCGACGGTTTGC
TGCATGAAATGGCCAGTCCGCCTCACGCCCTCTCGAGCCGCTTGTCGGCCCGTATCAAGGTGATGTCTCACCTTGATATA
GCGGAAAAGCGAAAGCCACAGGATGGCCGCATTAAGCTGAAAATATCCGAACGCAAGAGCATAGACTTTCGTGTCAGTAC
CCTGCCTACCATGTGGGGTGAGAAAATCGTGATGCGTATCCTCGATTCATCCGCCGCCAAACTGGGCATTGAGGCTCTGG
GCTATGAAGCAGATCAAAAGAGCATGTATCTAAATGCACTCGATCAGCCGCAGGGGATGATCCTGGTCACCGGACCGACA
GGTTCAGGTAAAACAGTCTCGCTTTACACCGGCCTGAATATACTCAATAAACCAGAACGCAACATCAGTACCGCCGAAGA
TCCGGTCGAAATTAACCTGGAAGGCATCAACCAGGTTCAAATCAATACGAAAGCGGACATGACCTTTGCCAACGCGCTGC
GGGCATTCCTGCGCCAGGATCCCGATGTGGTCATGGTCGGTGAGATCCGTGATCTGGAAACTGCGGAAATCGCCATCAAG
GCTGCACAAACTGGTCACCTTGTGATGAGCACACTGCATACCAATTCAGCTGCAGAGACCTTAACTCGCTTACTCAATAT
GGGCGTGCCGGCCTATAATGTGGCCAGCTCTGTCAGCCTGATCATTGCCCAGCGTCTTGCGCGTCGACTGTGTCCCAAAT
GCAAAGAGCCTGAACAATTGCCTGAAGAGGAATTGCAGCGTCAGGGTTTTAGCGCAGAACAAATTGCAAATATGACACTC
TATGCGCCCAAAGGGTGCGATCACTGCACCGATGGCTATAAAGGCCGGGTAGGTATTTATGAAGTGATGAAGATCACACC
CGAGATCTCGCATGCAATCATGGAAGGTGGTAACTCACTGGAGATTGCTGAACTTGCCGCGAGCAAGGGCTTTGCAAACC
TGCGCCAATCCGGGTTAAAAAAAGCTGCAGATGGGATCACCTCACTGGCTGAAGTCAACCGGGTCACGAACTATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-141)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0U3IIT1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.074

96.774

0.572
  pilB Acinetobacter baylyi ADP1

59.136

91.219

0.539
  pilB Vibrio cholerae strain A1552

54.083

98.746

0.534
  pilB Vibrio parahaemolyticus RIMD 2210633

54.328

97.312

0.529
  pilB Vibrio campbellii strain DS40M4

53.114

97.849

0.52
  pilB Legionella pneumophila strain ERS1305867

55.662

93.369

0.52
  pilF Neisseria gonorrhoeae MS11

47.491

100

0.475
  pilF Thermus thermophilus HB27

37.77

99.642

0.376
  pilB/pilB1 Synechocystis sp. PCC 6803

39.265

92.652

0.364

Multiple sequence alignment