Detailed information NTG0163212

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AT705_RS09720
• Genome accession: NZ_CP013611
• Coordinates: 2199417..2200292 (+)
• Length: 291 a.a.
• NCBI ID: WP_058796438.1
• Uniprot ID: A0A0U3GEC3
• Organism: Pseudoalteromonas rubra strain SCSIO 6842
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2194417..2205292

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AT705_RS09695 (AT705_09695)	ampE	2194785..2195669 (-)	885	WP_058796433.1	beta-lactamase regulator AmpE	-
AT705_RS09700 (AT705_09700)	ampD	2195681..2196217 (-)	537	WP_058796434.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
AT705_RS09705 (AT705_09705)	nadC	2196407..2197246 (+)	840	WP_058796435.1	carboxylating nicotinate-nucleotide diphosphorylase	-
AT705_RS26010 (AT705_09710)	-	2197644..2198108 (+)	465	WP_058796436.1	pilin	-
AT705_RS09715 (AT705_09715)	pilC	2198162..2199394 (+)	1233	WP_058796437.1	type II secretion system F family protein	Machinery gene
AT705_RS09720 (AT705_09720)	pilD	2199417..2200292 (+)	876	WP_058796438.1	prepilin peptidase	Machinery gene
AT705_RS09725 (AT705_09725)	coaE	2200296..2200907 (+)	612	WP_058796439.1	dephospho-CoA kinase	-
AT705_RS09730 (AT705_09730)	pilB	2201016..2202692 (+)	1677	WP_058796440.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AT705_RS09735 (AT705_09735)	yacG	2202764..2203006 (+)	243	WP_058796441.1	DNA gyrase inhibitor YacG	-
AT705_RS25485 (AT705_09740)	-	2203270..2203779 (-)	510	WP_058796442.1	Spy/CpxP family protein refolding chaperone	-
AT705_RS09745 (AT705_09745)	-	2203987..2204673 (+)	687	WP_058796443.1	response regulator transcription factor	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 32193.49 Da
• Isoelectric Point: 7.6384

>NTDB_id=163212 AT705_RS09720 WP_058796438.1 2199417..2200292(+) (pilD) [Pseudoalteromonas rubra strain SCSIO 6842]
MQEVWHLMEQQTWFWLLTVALTSLCIGSFLNVVIYRLPVMMQRNWQAECRIILEQAPQQAEPATFNLMTPGSTCPKCNTK
IKLHHNIPVLSWLLLGGKCAYCDAPIPKRYPLVELLTGAASLWLAWHFSATPQALVYILVTWVLIALIFIDIDHMLLPDQ
LTLPLLWFALLAAVAGLTIPPSAAIIGAAVGYLSLWSIYWVFKLLTGKEGMGFGDFKLLAVFGALMGWQSLLLIVLLSSV
VGAVIGATQLAVQGKDKASPIPFGPYLAIAGWLTLLYGEQLSRWYLSLLGV

Nucleotide

• Length: 876 bp

>NTDB_id=163212 AT705_RS09720 WP_058796438.1 2199417..2200292(+) (pilD) [Pseudoalteromonas rubra strain SCSIO 6842]
ATGCAAGAAGTTTGGCACCTTATGGAGCAGCAAACCTGGTTTTGGTTGCTGACAGTCGCACTGACCAGTTTGTGTATCGG
CAGCTTTTTAAACGTTGTCATTTATCGCTTACCCGTTATGATGCAGCGCAACTGGCAGGCCGAATGCCGGATCATTCTGG
AGCAGGCACCACAGCAAGCAGAACCTGCCACTTTTAATCTGATGACTCCCGGCTCTACCTGCCCTAAATGTAATACTAAG
ATTAAACTCCATCACAATATTCCCGTGCTCAGCTGGTTATTACTCGGTGGCAAATGCGCATACTGTGATGCACCAATTCC
CAAACGTTACCCGTTGGTTGAGCTACTCACTGGGGCGGCATCGCTCTGGCTGGCCTGGCATTTCTCAGCCACGCCTCAGG
CACTGGTGTACATTCTGGTCACCTGGGTATTAATCGCCCTGATCTTCATTGATATCGATCATATGCTGCTCCCCGATCAG
CTAACCCTCCCCTTACTCTGGTTTGCCCTGTTGGCGGCCGTGGCAGGCTTAACCATTCCTCCATCCGCAGCCATCATAGG
TGCGGCCGTTGGTTATCTCAGTCTGTGGAGCATTTACTGGGTATTCAAGCTACTGACCGGCAAAGAAGGCATGGGGTTTG
GTGATTTTAAGCTCTTAGCCGTATTTGGTGCTCTGATGGGCTGGCAGTCACTGTTGCTCATTGTGTTGCTATCAAGTGTT
GTTGGCGCGGTGATCGGCGCAACACAGCTCGCTGTGCAAGGTAAAGATAAGGCCAGCCCTATCCCCTTTGGCCCTTACCT
CGCGATTGCCGGCTGGCTAACGCTATTATATGGCGAGCAACTCAGCCGCTGGTATCTGTCGTTATTAGGAGTATAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (139-246)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-128)

3D structure

• AlphaFold DB: A0A0U3GEC3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.986	94.845	0.512
pilD	Vibrio campbellii strain DS40M4	51.042	98.969	0.505
pilD	Acinetobacter baumannii D1279779	48.432	98.625	0.478
pilD	Acinetobacter nosocomialis M2	48.084	98.625	0.474
pilD	Neisseria gonorrhoeae MS11	43.911	93.127	0.409