Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PI2015_RS13610 Genome accession   NZ_CP013350
Coordinates   2956476..2957705 (+) Length   409 a.a.
NCBI ID   WP_058155276.1    Uniprot ID   -
Organism   Pseudoalteromonas issachenkonii strain KCTC 12958     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2951476..2962705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PI2015_RS13590 (PI2015_2644) ampE 2952739..2953590 (-) 852 WP_058155273.1 beta-lactamase regulator AmpE -
  PI2015_RS13595 (PI2015_2645) ampD 2953878..2954423 (-) 546 WP_058155274.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PI2015_RS13600 (PI2015_2646) nadC 2954615..2955460 (+) 846 WP_058155275.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PI2015_RS13605 (PI2015_2647) - 2955968..2956402 (+) 435 WP_157066903.1 pilin -
  PI2015_RS13610 (PI2015_2648) pilC 2956476..2957705 (+) 1230 WP_058155276.1 type II secretion system F family protein Machinery gene
  PI2015_RS13615 (PI2015_2649) pilD 2957903..2958814 (+) 912 WP_058155277.1 prepilin peptidase Machinery gene
  PI2015_RS13620 (PI2015_2650) coaE 2958817..2959446 (+) 630 WP_058155278.1 dephospho-CoA kinase -
  PI2015_RS13625 (PI2015_2651) pilB 2959580..2961256 (+) 1677 WP_024601729.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PI2015_RS13630 (PI2015_2652) yacG 2961321..2961551 (+) 231 WP_024601728.1 DNA gyrase inhibitor YacG -
  PI2015_RS13635 (PI2015_2653) - 2961611..2962099 (-) 489 WP_036956691.1 Spy/CpxP family protein refolding chaperone -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44449.38 Da        Isoelectric Point: 9.4273

>NTDB_id=162212 PI2015_RS13610 WP_058155276.1 2956476..2957705(+) (pilC) [Pseudoalteromonas issachenkonii strain KCTC 12958]
MAKKQEDKGLDTFQWIGVSARGKKLEGELTGSSIALVKAQLRKQGITPSKVKRKAKPLFGLQSVQKITPKDIALVTRQIA
TMLMAGVPLIQSIEMIGSGSTNKSVSKLMETIGDEVRAGQPLSTALRKHPRYFDDLYCDLVASGEQSGALDKIFDRVALY
KEKSEALKSKIKKAMFYPIAVLVVALIVTSILLIFVVPQFQDIFNGFGAELPAFTLFVIAISEFMQEYWWMALIAVVAFG
YVFKETHLRSLALRDATDRAILKLPIIGMILNKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNAVYRYAILEIKAEV
SSGNQMNWAMRNSKIFPDMVIQMVAIGEESGSLDGMLAKVATIYEQEVDDAVDGLSSLLEPLIMAVLGVLVGGLIVAMYL
PIFQLGSVI

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=162212 PI2015_RS13610 WP_058155276.1 2956476..2957705(+) (pilC) [Pseudoalteromonas issachenkonii strain KCTC 12958]
ATGGCTAAAAAACAAGAAGATAAAGGCTTAGACACCTTCCAGTGGATTGGTGTGAGCGCTCGCGGCAAAAAACTCGAGGG
AGAACTTACCGGCTCAAGCATCGCTTTAGTCAAAGCGCAACTGCGTAAACAAGGCATTACCCCCTCAAAAGTAAAACGTA
AAGCTAAACCGTTATTTGGTTTGCAAAGTGTACAAAAAATCACCCCAAAAGATATTGCTTTAGTGACCAGGCAAATCGCG
ACTATGCTCATGGCGGGTGTGCCGCTGATTCAATCAATTGAAATGATAGGTTCTGGTTCCACCAATAAAAGCGTGAGCAA
GCTGATGGAAACCATTGGCGATGAAGTAAGAGCTGGCCAGCCGCTATCAACCGCGCTTAGAAAGCACCCTCGTTATTTTG
ATGATTTATATTGCGACTTAGTGGCCTCAGGTGAGCAGTCGGGCGCATTGGATAAAATATTCGACCGCGTTGCGCTCTAT
AAAGAAAAATCAGAGGCACTAAAATCAAAAATCAAAAAAGCCATGTTTTACCCGATTGCGGTATTAGTAGTAGCGCTGAT
TGTAACCTCCATATTACTTATTTTTGTGGTACCACAATTTCAGGATATTTTTAATGGCTTTGGCGCTGAGCTGCCTGCTT
TTACCTTATTTGTTATTGCTATTTCTGAATTTATGCAAGAGTACTGGTGGATGGCGCTAATAGCTGTTGTTGCTTTTGGT
TATGTATTCAAAGAAACCCATTTAAGAAGTTTAGCACTTAGGGATGCCACCGACCGAGCTATACTCAAACTGCCTATTAT
AGGTATGATACTTAATAAAGCAGCGGTAGCACGCTATGCGCGTACTTTATCGACCACCTTTGCTGCCGGTGTACCACTGG
TAGATGCGCTTGATTCTGCAGCTGGTGCCTCAGGAAACGCAGTTTACCGTTATGCTATTTTAGAAATAAAAGCCGAGGTA
AGCTCAGGTAACCAAATGAATTGGGCGATGCGTAATTCAAAAATATTCCCTGATATGGTAATTCAAATGGTTGCCATTGG
TGAAGAATCAGGATCGCTTGATGGCATGCTGGCTAAAGTGGCCACTATTTATGAGCAAGAAGTGGATGACGCCGTAGATG
GCCTATCTAGCCTATTAGAGCCATTAATAATGGCGGTACTCGGCGTATTAGTGGGTGGCTTAATTGTGGCCATGTACTTA
CCTATATTCCAACTAGGTTCAGTTATTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(280-401)

Type II secretion system (T2SS), protein F (T2SSF)

(76-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

62.72

97.066

0.609
  pilC Legionella pneumophila strain ERS1305867

55.25

97.8

0.54
  pilC Acinetobacter baumannii D1279779

55.25

97.8

0.54
  pilC Acinetobacter baylyi ADP1

55

97.8

0.538
  pilC Vibrio cholerae strain A1552

45.522

98.289

0.447
  pilG Neisseria meningitidis 44/76-A

44.246

95.599

0.423
  pilC Vibrio campbellii strain DS40M4

42.928

98.533

0.423
  pilG Neisseria gonorrhoeae MS11

43.99

95.599

0.421

Multiple sequence alignment