Detailed information NTG0162213

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PI2015_RS13615
• Genome accession: NZ_CP013350
• Coordinates: 2957903..2958814 (+)
• Length: 303 a.a.
• NCBI ID: WP_058155277.1
• Uniprot ID: -
• Organism: Pseudoalteromonas issachenkonii strain KCTC 12958
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2952903..2963814

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PI2015_RS13595 (PI2015_2645)	ampD	2953878..2954423 (-)	546	WP_058155274.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
PI2015_RS13600 (PI2015_2646)	nadC	2954615..2955460 (+)	846	WP_058155275.1	carboxylating nicotinate-nucleotide diphosphorylase	-
PI2015_RS13605 (PI2015_2647)	-	2955968..2956402 (+)	435	WP_157066903.1	pilin	-
PI2015_RS13610 (PI2015_2648)	pilC	2956476..2957705 (+)	1230	WP_058155276.1	type II secretion system F family protein	Machinery gene
PI2015_RS13615 (PI2015_2649)	pilD	2957903..2958814 (+)	912	WP_058155277.1	prepilin peptidase	Machinery gene
PI2015_RS13620 (PI2015_2650)	coaE	2958817..2959446 (+)	630	WP_058155278.1	dephospho-CoA kinase	-
PI2015_RS13625 (PI2015_2651)	pilB	2959580..2961256 (+)	1677	WP_024601729.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PI2015_RS13630 (PI2015_2652)	yacG	2961321..2961551 (+)	231	WP_024601728.1	DNA gyrase inhibitor YacG	-
PI2015_RS13635 (PI2015_2653)	-	2961611..2962099 (-)	489	WP_036956691.1	Spy/CpxP family protein refolding chaperone	-
PI2015_RS13640 (PI2015_2654)	-	2962267..2962956 (+)	690	WP_016899750.1	response regulator transcription factor	-

Sequence

Protein

• Length: 303 a.a.
• Molecular weight: 33399.74 Da
• Isoelectric Point: 7.2372

>NTDB_id=162213 PI2015_RS13615 WP_058155277.1 2957903..2958814(+) (pilD) [Pseudoalteromonas issachenkonii strain KCTC 12958]
MQSLFIDITAAMQNHLWFYLTTIGLVSLCIGSFLNVVIYRLPLMMQKEWQTECRLLLADELTSPKAKQTTEHTTDTFNLV
QPNSCCPKCKAAIKPWQNIPIISWLLLKGKCATCNNPISVRYPLVEAITAILSLVVAHTFGATEQALLYIVVTWALVALT
FIDIDHMLLPDQLTLPLLWLALIASVMGHTIAPSDAIIGAACGYLSLWSVFWLFKLITGKEGMGYGDFKLLAVFGALLGW
QSLLTIILLSSVVGAVIGIALLSIQGKDKATPIPFGPYLAIAGWITMLWGTQLQNSYFNFIGY

Nucleotide

• Length: 912 bp

>NTDB_id=162213 PI2015_RS13615 WP_058155277.1 2957903..2958814(+) (pilD) [Pseudoalteromonas issachenkonii strain KCTC 12958]
ATGCAAAGTCTTTTTATTGATATCACCGCCGCGATGCAAAACCATTTATGGTTTTACTTAACCACTATTGGACTGGTGAG
TTTATGTATTGGTAGCTTTTTAAATGTGGTGATATATCGCTTACCGCTGATGATGCAAAAAGAATGGCAAACTGAATGCC
GCTTATTATTAGCCGATGAACTAACCAGCCCAAAGGCTAAACAGACCACTGAACACACTACTGACACGTTTAACTTAGTT
CAACCCAATTCATGTTGCCCAAAGTGCAAAGCGGCCATTAAACCGTGGCAAAATATCCCTATAATTAGCTGGTTATTATT
AAAAGGTAAATGCGCAACCTGTAACAACCCAATATCAGTACGTTACCCTCTCGTTGAGGCCATTACCGCTATTTTAAGTT
TAGTGGTTGCCCACACCTTTGGCGCTACAGAGCAGGCTTTATTGTACATTGTAGTTACTTGGGCGTTGGTAGCACTGACT
TTTATTGATATTGATCACATGCTACTGCCGGACCAGCTTACTTTGCCACTTTTATGGTTAGCACTAATAGCCTCAGTTAT
GGGTCATACCATTGCCCCTAGCGATGCCATTATTGGCGCAGCATGTGGCTATTTAAGCTTATGGAGTGTGTTTTGGTTGT
TTAAATTAATAACCGGCAAAGAAGGCATGGGCTATGGCGACTTTAAATTACTTGCGGTGTTTGGCGCATTGCTAGGTTGG
CAATCGTTATTGACCATTATTTTGCTCTCAAGCGTGGTTGGCGCAGTAATTGGCATTGCTCTACTGAGCATTCAAGGTAA
AGACAAAGCAACCCCTATTCCTTTTGGCCCTTATTTAGCAATAGCCGGTTGGATCACTATGCTATGGGGAACGCAACTGC
AAAACAGCTATTTTAATTTCATTGGCTACTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (26-140)

• Type IV leader peptidase family (Peptidase_A24) (151-259)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	50.853	96.7	0.492
pilD	Vibrio cholerae strain A1552	51.568	94.719	0.488
pilD	Acinetobacter nosocomialis M2	50.512	96.7	0.488
pilD	Vibrio campbellii strain DS40M4	50.859	96.04	0.488
pilD	Neisseria gonorrhoeae MS11	45.149	88.449	0.399