Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ATY38_RS05830 Genome accession   NZ_CP013341
Coordinates   1262790..1263926 (-) Length   378 a.a.
NCBI ID   WP_062558482.1    Uniprot ID   A0A1H2DNC7
Organism   Nitrosomonas ureae strain Nm10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1257790..1268926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ATY38_RS05805 (ATY38_05800) recX 1257851..1258309 (+) 459 WP_074701413.1 recombination regulator RecX -
  ATY38_RS05810 (ATY38_05805) - 1258354..1258671 (+) 318 WP_235590400.1 hypothetical protein -
  ATY38_RS05815 (ATY38_05810) alaS 1258668..1261265 (+) 2598 WP_062558479.1 alanine--tRNA ligase -
  ATY38_RS05820 (ATY38_05815) - 1261522..1261710 (+) 189 WP_062558480.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  ATY38_RS05825 (ATY38_05820) - 1261724..1262638 (+) 915 WP_062558481.1 hypothetical protein -
  ATY38_RS05830 (ATY38_05825) pilU 1262790..1263926 (-) 1137 WP_062558482.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ATY38_RS05835 (ATY38_05830) pilT 1263967..1265010 (-) 1044 WP_062558483.1 type IV pilus twitching motility protein PilT Machinery gene
  ATY38_RS05840 (ATY38_05835) - 1265277..1266026 (+) 750 WP_082632977.1 IS5 family transposase -
  ATY38_RS05845 (ATY38_05840) sbcB 1266238..1267674 (-) 1437 WP_062558484.1 exodeoxyribonuclease I -
  ATY38_RS05850 (ATY38_05845) - 1267790..1268182 (+) 393 WP_062558485.1 RidA family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41962.52 Da        Isoelectric Point: 7.2877

>NTDB_id=162159 ATY38_RS05830 WP_062558482.1 1262790..1263926(-) (pilU) [Nitrosomonas ureae strain Nm10]
MNKEQAAKFMSDLLRLMLSKKASDLFITAGFPPAFKIDGKMTPVSQQSLTAQHTEMLAKAIMNEKQVGEFKTSQECNFAI
NPAGIGRFRVNAFVQQQRVGMVLRTITTKIPEFDDLALPQVLKEVVMSKRGLVIFVGGTGSGKSTSMAALIGHRNKNSYG
HIITIEDPVEYVHEHINCVITQREVGVDTESWEAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCMGTLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVAQRLIPAKDSKGRVAAIEVLLNSPLISDLIFKGNVHEIKTIMKSSREMG
MQTFDMALFNLYEAGKISYEDALRNADSMNELRLQIKLEGAEAKTHDLNSGIAHLAIT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=162159 ATY38_RS05830 WP_062558482.1 1262790..1263926(-) (pilU) [Nitrosomonas ureae strain Nm10]
GTGAATAAAGAACAAGCTGCAAAATTTATGTCGGATTTACTGCGCTTAATGCTGAGCAAGAAAGCCTCGGATTTGTTTAT
TACAGCCGGTTTCCCGCCTGCATTCAAAATCGATGGAAAAATGACGCCGGTCTCTCAGCAATCCCTGACTGCACAGCACA
CCGAAATGTTAGCCAAAGCGATCATGAATGAAAAACAAGTCGGCGAATTTAAAACCTCGCAGGAATGCAATTTTGCAATT
AACCCAGCGGGAATCGGGCGTTTCCGTGTTAATGCGTTTGTCCAGCAACAACGCGTGGGTATGGTATTACGTACGATCAC
CACCAAAATCCCCGAGTTTGATGATTTGGCCCTGCCACAAGTGCTCAAGGAAGTTGTGATGAGCAAGCGCGGCCTGGTGA
TTTTTGTCGGCGGAACAGGTTCCGGAAAATCAACATCAATGGCAGCATTGATCGGGCATCGCAACAAGAACAGCTATGGC
CATATCATCACCATTGAAGATCCGGTTGAATATGTTCACGAGCACATTAACTGTGTCATCACCCAACGCGAAGTGGGTGT
CGACACCGAATCATGGGAAGCGGCGCTGAAAAACACACTGCGGCAAGCGCCCGATGTCATATTGATTGGCGAAATCCGTG
ATCGTGAAACCATGGAACACGCCATTGCATTTGCCGAAACCGGCCACCTTTGCATGGGAACATTGCATGCCAACAGCGCA
AACCAGGCGCTCGATCGTATCATTAATTTTTTCCCGGAAGAACGCAGGGCACAGTTATTGATGGATTTATCGCTGAATCT
GCGCTCATTGGTGGCACAACGGTTGATCCCGGCTAAAGACAGTAAAGGAAGAGTCGCGGCTATCGAAGTCCTGCTCAATT
CACCGTTGATTTCGGATTTGATTTTTAAGGGCAACGTGCATGAAATCAAAACCATCATGAAAAGCTCGCGCGAAATGGGC
ATGCAAACCTTTGATATGGCATTGTTTAACCTCTATGAAGCCGGAAAGATCAGTTATGAAGATGCGCTGCGCAATGCCGA
CTCGATGAATGAATTGCGCCTGCAAATCAAACTCGAAGGCGCCGAAGCTAAAACTCATGATCTTAATTCAGGGATTGCAC
ATCTGGCAATTACGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(19-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H2DNC7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.687

94.709

0.603
  pilU Acinetobacter baylyi ADP1

60.571

92.593

0.561
  pilU Vibrio cholerae strain A1552

56.305

90.212

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.62

89.153

0.389
  pilT Pseudomonas aeruginosa PAK

41.84

89.153

0.373
  pilT Legionella pneumophila strain Lp02

41.141

88.095

0.362
  pilT Legionella pneumophila strain ERS1305867

41.141

88.095

0.362

Multiple sequence alignment