Detailed information NTG0160725

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: HSISS4_RS09050
• Genome accession: NZ_CP013216
• Coordinates: 1962487..1962924 (-)
• Length: 145 a.a.
• NCBI ID: WP_021144684.1
• Uniprot ID: A0AAW6D9H3
• Organism: Streptococcus salivarius strain HSISS4
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1957487..1967924

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HSISS4_RS09015 (HSISS4_01755)	-	1957526..1958188 (-)	663	WP_021144681.1	CPBP family intramembrane glutamic endopeptidase	-
HSISS4_RS09020 (HSISS4_01756)	-	1958301..1958747 (-)	447	WP_021144682.1	hypothetical protein	-
HSISS4_RS09025 (HSISS4_01757)	-	1958825..1959481 (-)	657	WP_002887011.1	CPBP family intramembrane glutamic endopeptidase	-
HSISS4_RS09030 (HSISS4_01758)	-	1959493..1959690 (-)	198	WP_002885716.1	helix-turn-helix transcriptional regulator	-
HSISS4_RS09035 (HSISS4_01759)	-	1959941..1961134 (-)	1194	WP_002885831.1	acetate kinase	-
HSISS4_RS09040 (HSISS4_01760)	comYH	1961191..1962147 (-)	957	WP_002885753.1	class I SAM-dependent methyltransferase	Machinery gene
HSISS4_RS09045 (HSISS4_01761)	comGG	1962192..1962509 (-)	318	WP_021144683.1	competence type IV pilus minor pilin ComGG	-
HSISS4_RS09050 (HSISS4_01762)	comYF	1962487..1962924 (-)	438	WP_021144684.1	competence type IV pilus minor pilin ComGF	Machinery gene
HSISS4_RS09055 (HSISS4_01763)	comGE	1962911..1963141 (-)	231	WP_021144685.1	competence type IV pilus minor pilin ComGE	-
HSISS4_RS09060 (HSISS4_01764)	comYD	1963173..1963601 (-)	429	WP_253183813.1	competence type IV pilus minor pilin ComGD	Machinery gene
HSISS4_RS09065 (HSISS4_01765)	comYC	1963561..1963887 (-)	327	WP_002885815.1	competence type IV pilus major pilin ComGC	Machinery gene
HSISS4_RS09070 (HSISS4_01766)	comYB	1963884..1964984 (-)	1101	WP_144429890.1	competence type IV pilus assembly protein ComGB	Machinery gene
HSISS4_RS09075 (HSISS4_01767)	comYA	1964866..1965807 (-)	942	WP_002885658.1	competence type IV pilus ATPase ComGA	Machinery gene
HSISS4_RS09080 (HSISS4_01768)	-	1965889..1966251 (-)	363	WP_021144688.1	DUF1033 family protein	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16229.70 Da
• Isoelectric Point: 9.0226

>NTDB_id=160725 HSISS4_RS09050 WP_021144684.1 1962487..1962924(-) (comYF) [Streptococcus salivarius strain HSISS4]
MSLNSKVRAFTLLECLVALLVIAGSIQVYQGLTTVLVSNVKQVKQQENQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSATDFRKTNADGRGYQPMLFNVKSSTISQKSQIVTIQVTLKNGLQRSFVYAFEKTS

Nucleotide

• Length: 438 bp

>NTDB_id=160725 HSISS4_RS09050 WP_021144684.1 1962487..1962924(-) (comYF) [Streptococcus salivarius strain HSISS4]
ATGTCTCTCAATAGCAAGGTTCGTGCTTTTACCCTCTTGGAATGTCTAGTAGCTCTATTGGTGATTGCTGGTTCAATTCA
AGTTTATCAAGGTCTGACCACAGTATTGGTGAGCAATGTTAAGCAGGTCAAGCAACAAGAAAACCAAGACTGGCTCCTAT
TTGTTCAACAAATGGAGGCAGAATTAGAAGGTTGTCAGTTGGTAAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACTTAGCTTTTGGACTATCAAGTGCTACTGATTTTCGAAAAACAAATGCTGATGGTCGAGGTTACCAACCTAT
GCTTTTTAATGTAAAATCTAGTACGATAAGCCAAAAGAGCCAGATAGTCACCATCCAAGTGACTCTTAAAAATGGGCTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAAGTTAG

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	56.738	97.241	0.552
comYF	Streptococcus mutans UA159	56.028	97.241	0.545
comGF/cglF	Streptococcus pneumoniae D39	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae Rx1	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae R6	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae TIGR4	50	97.931	0.49
comGF/cglF	Streptococcus mitis NCTC 12261	47.222	99.31	0.469
comGF/cglF	Streptococcus mitis SK321	47.222	99.31	0.469
comGF	Lactococcus lactis subsp. cremoris KW2	44.604	95.862	0.428