Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WL1483_RS03255 Genome accession   NZ_CP013067
Coordinates   647525..648643 (+) Length   372 a.a.
NCBI ID   WP_060584086.1    Uniprot ID   A0A0S2SES6
Organism   Aeromonas schubertii strain WL1483     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 642525..653643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WL1483_RS03215 (WL1483_1000) hupA 642761..643033 (+) 273 WP_005351739.1 nucleoid-associated protein HU-alpha -
  WL1483_RS03220 (WL1483_2451) - 643141..643557 (+) 417 WP_050667360.1 hypothetical protein -
  WL1483_RS03225 (WL1483_1659) - 643609..644028 (-) 420 WP_050667361.1 DUF4426 domain-containing protein -
  WL1483_RS03230 (WL1483_3994) yggU 644057..644347 (-) 291 WP_060584082.1 DUF167 family protein YggU -
  WL1483_RS03235 (WL1483_3506) - 644347..644898 (-) 552 WP_050667363.1 YggT family protein -
  WL1483_RS03240 (WL1483_354) proC 644908..645729 (-) 822 WP_060584084.1 pyrroline-5-carboxylate reductase -
  WL1483_RS03245 (WL1483_4144) - 645741..646442 (-) 702 WP_060584085.1 YggS family pyridoxal phosphate-dependent enzyme -
  WL1483_RS03250 pilT 646482..647516 (+) 1035 WP_050667366.1 type IVa pilus ATPase TapT Machinery gene
  WL1483_RS03255 (WL1483_727) pilU 647525..648643 (+) 1119 WP_060584086.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WL1483_RS03260 (WL1483_3261) yaaA 648658..649437 (+) 780 WP_060584087.1 peroxide stress protein YaaA -
  WL1483_RS03265 (WL1483_1709) srmB 649581..650810 (-) 1230 WP_050667369.1 ATP-dependent RNA helicase SrmB -
  WL1483_RS03270 (WL1483_5013) - 650925..651632 (+) 708 WP_050667370.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  WL1483_RS03275 (WL1483_763) brnQ 651867..653129 (+) 1263 Protein_617 branched-chain amino acid transport system II carrier protein -
  WL1483_RS03280 (WL1483_294) - 653240..653476 (-) 237 WP_060584090.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41200.42 Da        Isoelectric Point: 6.1358

>NTDB_id=159531 WL1483_RS03255 WP_060584086.1 647525..648643(+) (pilU) [Aeromonas schubertii strain WL1483]
MSMDAWLGELVARRGSDLFVTTGMAPTVKQDGRFVALGDTPLSAKEVEEAMALIMDEESRHLFAQTREANFAIQRPGLGR
FRVSTFLQLAQPGMVLRRIETRIPTFEELKLPPVLREVVMAKRGLILFVGATGAGKSTTQAAMIGYRNQQSDGHILTVED
PVEFVHHHGRSIVTQREVGLDTPSFDSALHSALRQAPDLILIGEIRTQETMEFAIQFAETGHICMATLHANNADQAIERI
MHLMPEGRHRQLRYDLAFNLKAIVAQQLLPLKSGSGRSAAFEILLNTPLIADIIRKGEVDRLKDVMGRSREQGMVTFDQS
LFDLYMGGQIDMETALAHADSVNDLRLMIKLKGGEKLGSGSLDNVTLQPDME

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=159531 WL1483_RS03255 WP_060584086.1 647525..648643(+) (pilU) [Aeromonas schubertii strain WL1483]
ATGAGCATGGATGCCTGGTTGGGCGAGCTGGTGGCGAGGCGTGGTTCGGATCTCTTCGTCACGACTGGCATGGCCCCTAC
CGTGAAGCAGGACGGGCGCTTCGTCGCCCTCGGCGATACGCCCTTGTCGGCAAAAGAGGTTGAAGAGGCGATGGCCCTCA
TCATGGATGAGGAGAGCAGACACCTCTTTGCACAGACCAGGGAGGCCAACTTCGCCATACAGAGGCCGGGTCTCGGTCGT
TTCCGGGTCAGTACTTTCCTGCAACTGGCCCAGCCTGGCATGGTGCTGCGTCGCATCGAGACCCGGATCCCCACCTTCGA
AGAGCTCAAGCTGCCACCTGTGCTCAGGGAGGTCGTGATGGCCAAGCGGGGGCTGATCCTCTTCGTCGGTGCCACCGGTG
CCGGTAAGTCGACGACCCAGGCGGCCATGATCGGCTATCGCAATCAGCAGAGTGATGGCCACATCCTGACGGTCGAAGAT
CCGGTGGAATTTGTGCATCACCACGGGCGCAGCATCGTGACCCAGCGAGAGGTGGGGCTGGATACGCCCTCTTTCGATTC
GGCCCTGCACAGTGCCCTGCGTCAGGCGCCGGACCTTATCCTGATCGGCGAGATCCGTACCCAGGAGACCATGGAGTTTG
CCATCCAGTTTGCCGAGACGGGCCATATCTGCATGGCCACTCTGCACGCCAACAATGCTGACCAGGCGATCGAACGGATC
ATGCATCTGATGCCGGAGGGGCGGCACCGCCAGCTGCGCTACGATCTGGCGTTCAATCTGAAGGCCATCGTCGCCCAGCA
GTTGCTGCCCCTCAAGAGTGGTAGTGGGCGCAGTGCTGCGTTCGAGATCCTGCTCAACACGCCCCTGATTGCCGACATCA
TCCGTAAAGGAGAGGTGGACAGACTCAAGGATGTGATGGGGCGCTCCCGCGAACAGGGGATGGTGACCTTCGATCAATCC
CTCTTCGATCTCTACATGGGGGGACAGATCGACATGGAGACGGCCCTGGCCCATGCGGACTCGGTGAACGATCTCCGGTT
GATGATCAAGCTGAAGGGGGGTGAGAAACTTGGCTCCGGCTCTCTGGATAATGTTACCCTGCAGCCCGATATGGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0S2SES6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

58.333

100

0.583
  pilU Pseudomonas stutzeri DSM 10701

57.418

97.849

0.562
  pilU Acinetobacter baylyi ADP1

50.938

100

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.795

85.215

0.382
  pilT Acinetobacter baylyi ADP1

40.176

91.667

0.368
  pilT Legionella pneumophila strain Lp02

40.299

90.054

0.363
  pilT Legionella pneumophila strain ERS1305867

40.299

90.054

0.363

Multiple sequence alignment