Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WL1483_RS03250 Genome accession   NZ_CP013067
Coordinates   646482..647516 (+) Length   344 a.a.
NCBI ID   WP_050667366.1    Uniprot ID   -
Organism   Aeromonas schubertii strain WL1483     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 641482..652516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WL1483_RS03210 (WL1483_1153) rpoS 641561..642544 (+) 984 WP_060584075.1 RNA polymerase sigma factor RpoS -
  WL1483_RS03215 (WL1483_1000) hupA 642761..643033 (+) 273 WP_005351739.1 nucleoid-associated protein HU-alpha -
  WL1483_RS03220 (WL1483_2451) - 643141..643557 (+) 417 WP_050667360.1 hypothetical protein -
  WL1483_RS03225 (WL1483_1659) - 643609..644028 (-) 420 WP_050667361.1 DUF4426 domain-containing protein -
  WL1483_RS03230 (WL1483_3994) yggU 644057..644347 (-) 291 WP_060584082.1 DUF167 family protein YggU -
  WL1483_RS03235 (WL1483_3506) - 644347..644898 (-) 552 WP_050667363.1 YggT family protein -
  WL1483_RS03240 (WL1483_354) proC 644908..645729 (-) 822 WP_060584084.1 pyrroline-5-carboxylate reductase -
  WL1483_RS03245 (WL1483_4144) - 645741..646442 (-) 702 WP_060584085.1 YggS family pyridoxal phosphate-dependent enzyme -
  WL1483_RS03250 pilT 646482..647516 (+) 1035 WP_050667366.1 type IVa pilus ATPase TapT Machinery gene
  WL1483_RS03255 (WL1483_727) pilU 647525..648643 (+) 1119 WP_060584086.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WL1483_RS03260 (WL1483_3261) yaaA 648658..649437 (+) 780 WP_060584087.1 peroxide stress protein YaaA -
  WL1483_RS03265 (WL1483_1709) srmB 649581..650810 (-) 1230 WP_050667369.1 ATP-dependent RNA helicase SrmB -
  WL1483_RS03270 (WL1483_5013) - 650925..651632 (+) 708 WP_050667370.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38354.07 Da        Isoelectric Point: 6.6938

>NTDB_id=159530 WL1483_RS03250 WP_050667366.1 646482..647516(+) (pilT) [Aeromonas schubertii strain WL1483]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEMRKINLPVLEHREVHALIYDIMNDQQRKELEENYEVDFSFEIPGLAR
FRVNAFQQSRGSGAVFRTIPSTVLSLDELDAPEVFRKIAEYPRGLVLVTGPTGSGKSTTLAAMINYINESFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMIGIPAVRNLIREDKVPQLYSVIQTGMTHGMQTMDQCL
KQLVGRGVVTQQDARGKAADPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=159530 WL1483_RS03250 WP_050667366.1 646482..647516(+) (pilT) [Aeromonas schubertii strain WL1483]
ATGGATATCACAGAGTTATTGGCCTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCTGCCGGGGTGCCACCCAT
GATCCGGGTCGACGGGGAGATGCGCAAGATCAATCTGCCCGTCCTGGAGCACAGGGAGGTTCACGCCCTCATCTATGACA
TCATGAACGATCAGCAGCGCAAGGAGCTTGAAGAGAATTACGAGGTCGACTTCTCCTTCGAGATCCCCGGGTTGGCGCGC
TTTCGTGTCAACGCCTTCCAGCAGTCTCGTGGCTCGGGAGCCGTCTTCCGGACTATCCCCAGTACAGTATTGAGTCTCGA
TGAGCTCGATGCTCCCGAGGTGTTTCGCAAGATCGCCGAATATCCCCGTGGTCTGGTACTGGTGACGGGGCCAACCGGCT
CGGGCAAGTCGACGACGCTGGCCGCCATGATCAACTACATCAACGAGTCCTTTCATCATCACATCCTCACCATCGAGGAT
CCCATCGAGTTCGTGCATGAGAACAAACGCTGTCTGGTGAACCAGCGCGAGGTGCACCGGGATACCAAGAGCTTCAGCAA
TGCCCTGCGCTCGGCCCTGCGTGAAGACCCCGACATCATACTGGTCGGTGAGATGCGGGATCTCGAAACCATTCGGCTGG
CGATGACGGCGGCCGAGACCGGTCACCTGGTGTTTGGCACTCTGCACACCTCGTCGGCGGCCAAGACCATAGACCGTATC
ATCGACGTCTTCCCCGGGGCCGAGAAGGACATGGTGCGCTCCATGTTGTCCGAATCCCTGAGGGCGGTTATCTCCCAGAC
CCTGCTCAAACGTATCGGCGGTGGTCGGGTGGCCGCACACGAGATCATGATAGGTATCCCGGCGGTGCGAAACCTGATCC
GGGAAGACAAGGTGCCCCAGCTCTACTCTGTGATTCAGACCGGGATGACACATGGCATGCAGACCATGGATCAGTGCCTC
AAGCAGTTGGTCGGGCGCGGCGTGGTTACCCAGCAGGATGCCAGGGGCAAGGCCGCCGATCCCAACAGTATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.163

100

0.762
  pilT Acinetobacter baylyi ADP1

76.163

100

0.762
  pilT Acinetobacter baumannii D1279779

75.581

100

0.756
  pilT Acinetobacter baumannii strain A118

75.581

100

0.756
  pilT Acinetobacter nosocomialis M2

75.581

100

0.756
  pilT Pseudomonas stutzeri DSM 10701

75.581

100

0.756
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

74.481

97.965

0.73
  pilT Vibrio cholerae strain A1552

74.481

97.965

0.73
  pilT Legionella pneumophila strain Lp02

71.893

98.256

0.706
  pilT Legionella pneumophila strain ERS1305867

71.893

98.256

0.706
  pilT Neisseria meningitidis 8013

69.275

100

0.695
  pilT Neisseria gonorrhoeae MS11

68.986

100

0.692
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.632

99.419

0.523
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

41.739

100

0.419
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407

Multiple sequence alignment