Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AR383_RS08745 Genome accession   NZ_CP013021
Coordinates   1876491..1878197 (+) Length   568 a.a.
NCBI ID   WP_055732785.1    Uniprot ID   -
Organism   Agarivorans gilvus strain WH0801     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1871491..1883197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AR383_RS08720 pdhR 1872293..1873057 (-) 765 WP_055732781.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AR383_RS08725 ampE 1873300..1874154 (-) 855 WP_055732782.1 regulatory signaling modulator protein AmpE -
  AR383_RS08730 ampD 1874232..1874771 (-) 540 WP_083481554.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AR383_RS08735 nadC 1874948..1875790 (+) 843 WP_055732783.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AR383_RS22010 - 1876031..1876483 (+) 453 WP_055732784.1 pilin -
  AR383_RS08745 pilB 1876491..1878197 (+) 1707 WP_055732785.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AR383_RS08750 pilC 1878284..1879534 (+) 1251 WP_055732786.1 type II secretion system F family protein Machinery gene
  AR383_RS08755 pilD 1879537..1880415 (+) 879 WP_055732787.1 prepilin peptidase Machinery gene
  AR383_RS08760 coaE 1880415..1881017 (+) 603 WP_055732788.1 dephospho-CoA kinase -
  AR383_RS08765 zapD 1881076..1881816 (+) 741 WP_055732789.1 cell division protein ZapD -
  AR383_RS08770 yacG 1881816..1882016 (+) 201 WP_055732790.1 DNA gyrase inhibitor YacG -
  AR383_RS08775 mutT 1882013..1882423 (-) 411 WP_055732791.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62860.02 Da        Isoelectric Point: 4.9393

>NTDB_id=159302 AR383_RS08745 WP_055732785.1 1876491..1878197(+) (pilB) [Agarivorans gilvus strain WH0801]
MQQSASLNGLASSLAVSFPEKEQQLLELYKRSQQEQSAFTTVLVENELFTSKALADYCEAQFGIPLLDLDAIEINEIPQS
FLNEKLIFKHHVLPVYSQNQTLYVAMADPSNVSALEDFGFSFGLHVEALLVEENKLSKAIQSLVDDSANLDLGDISDEDI
NDLEVSEENSRLDEKQNNKEDDAPIVVYINKILMDSIRRGASDLHFEPYEHKYRIRFRIDGILHEIASPPINLANRFSAR
LKVMARMDIAERRLPQDGRIKLKTGRNKAIDMRVNSLPTMWGEKIVIRILDSSAANLNIDILGYDDKQKQLYLNALSRPQ
GMILVTGPTGSGKTVSLYTGLNILNTVETNISTAEDPVEINLPGVNQVQINNKAGLTFAAALRAFLRQDPDIVMVGEIRD
LETAEISIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPAFNIASSVSLIIAQRLARRLCEHCKKPKMLEAELLPKIGFS
AEQIAQGIKLYEPVGCDKCTGGFKGRVGIYEVMPMSSEIAKLIMAEGNSIQIAEQAINEGMYSLRMSALEKARIGVTSLS
EVERVTNV

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=159302 AR383_RS08745 WP_055732785.1 1876491..1878197(+) (pilB) [Agarivorans gilvus strain WH0801]
ATGCAACAATCAGCCTCACTAAACGGCCTAGCTTCTAGCTTGGCCGTTTCTTTTCCAGAAAAAGAGCAACAGCTGCTGGA
GCTGTACAAGCGCAGCCAGCAAGAGCAGAGTGCCTTTACTACGGTATTGGTAGAAAACGAGTTGTTTACCAGTAAGGCGC
TCGCTGATTATTGCGAGGCCCAGTTCGGCATTCCGCTGCTGGATCTCGATGCCATCGAGATCAACGAAATTCCCCAGAGT
TTTCTCAACGAAAAGCTGATTTTTAAACACCACGTGCTGCCGGTGTATAGTCAAAACCAAACCCTATATGTGGCCATGGC
TGACCCAAGTAATGTAAGCGCGCTAGAAGACTTTGGTTTTAGCTTTGGCCTACACGTAGAAGCACTGTTGGTTGAGGAAA
ATAAACTCAGCAAAGCGATTCAGTCACTGGTGGATGATAGCGCCAACCTCGACTTGGGTGATATTTCCGATGAGGACATC
AACGACCTAGAAGTTAGCGAAGAAAACTCTCGGCTCGACGAAAAACAAAATAATAAAGAAGACGATGCGCCCATCGTTGT
TTATATTAATAAAATTTTAATGGACTCGATCCGCCGTGGCGCATCGGATTTGCATTTCGAGCCCTACGAGCATAAATACC
GAATTCGTTTTCGCATCGACGGCATATTGCACGAAATCGCCAGCCCGCCAATCAATTTGGCCAACCGCTTCTCGGCTCGC
CTCAAAGTAATGGCGCGTATGGACATCGCCGAGCGACGCTTACCGCAAGATGGCCGTATTAAGCTTAAAACCGGCCGCAA
CAAAGCCATCGACATGCGGGTTAACTCCTTGCCCACCATGTGGGGCGAGAAAATTGTGATTCGTATTTTGGACTCCTCGG
CCGCCAACCTGAATATCGATATATTGGGTTACGACGATAAACAAAAGCAGCTTTACCTAAACGCGCTGTCTCGCCCGCAG
GGGATGATACTAGTCACCGGCCCCACCGGCTCAGGTAAAACCGTATCGCTGTATACTGGCCTAAATATTTTGAATACCGT
GGAAACCAATATTTCTACCGCAGAAGATCCGGTGGAAATTAACTTACCTGGGGTTAACCAAGTTCAAATTAATAACAAGG
CCGGATTAACTTTTGCCGCCGCGCTGCGCGCCTTTCTTCGTCAAGATCCCGACATTGTAATGGTGGGAGAAATCCGTGAC
TTAGAAACCGCCGAAATTTCGATTAAGGCGGCGCAAACTGGTCACTTAGTCTTATCCACCCTGCATACCAATTCAGCCGC
CGAAACCTTGACCCGTTTACTGAATATGGGAGTGCCGGCCTTTAACATTGCTTCTTCGGTGAGTTTGATTATTGCTCAGC
GCTTGGCACGCCGCCTCTGCGAGCATTGTAAAAAACCAAAAATGCTAGAAGCTGAATTGCTGCCTAAGATTGGCTTTAGC
GCAGAGCAAATAGCCCAAGGCATTAAACTCTACGAACCCGTGGGCTGTGATAAATGCACCGGTGGCTTTAAGGGCCGCGT
GGGTATTTACGAAGTGATGCCCATGAGTAGTGAAATCGCTAAACTGATTATGGCCGAAGGCAACTCTATACAAATTGCCG
AACAAGCCATTAACGAAGGCATGTATTCACTGCGTATGTCAGCCTTAGAGAAGGCGCGTATAGGAGTCACCTCTTTATCG
GAAGTGGAGCGGGTCACAAATGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.204

96.479

0.542
  pilB Acinetobacter baylyi ADP1

55.311

96.127

0.532
  pilB Vibrio cholerae strain A1552

54.197

96.479

0.523
  pilB Legionella pneumophila strain ERS1305867

52.482

99.296

0.521
  pilB Vibrio campbellii strain DS40M4

52

96.831

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

53.992

92.606

0.5
  pilF Neisseria gonorrhoeae MS11

48.529

95.775

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

38.584

99.472

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.113

93.486

0.375
  pilF Thermus thermophilus HB27

36.348

100

0.368

Multiple sequence alignment