Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACT75_RS07360 Genome accession   NZ_CP012959
Coordinates   1509981..1511390 (-) Length   469 a.a.
NCBI ID   WP_005540457.1    Uniprot ID   Q8KRY5
Organism   Aggregatibacter actinomycetemcomitans strain 624     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1504981..1516390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACT75_RS07340 (ACT75_07330) - 1506574..1507701 (-) 1128 WP_005539200.1 DUF4123 domain-containing protein -
  ACT75_RS07350 (ACT75_07340) - 1508079..1508768 (-) 690 WP_005594882.1 prepilin peptidase -
  ACT75_RS07355 (ACT75_07345) pilC 1508765..1509988 (-) 1224 WP_061866560.1 type II secretion system F family protein Machinery gene
  ACT75_RS07360 (ACT75_07350) pilB 1509981..1511390 (-) 1410 WP_005540457.1 GspE/PulE family protein Machinery gene
  ACT75_RS07365 (ACT75_07355) pilA 1511417..1511869 (-) 453 WP_005550263.1 pilin Machinery gene
  ACT75_RS07370 (ACT75_07360) ampD 1511991..1512545 (+) 555 WP_005540460.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACT75_RS07375 (ACT75_07365) nanQ 1513011..1513484 (-) 474 WP_005594879.1 N-acetylneuraminate anomerase -
  ACT75_RS07380 (ACT75_07370) pnp 1513778..1515907 (+) 2130 WP_032997160.1 polyribonucleotide nucleotidyltransferase -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 52493.93 Da        Isoelectric Point: 6.3457

>NTDB_id=158613 ACT75_RS07360 WP_005540457.1 1509981..1511390(-) (pilB) [Aggregatibacter actinomycetemcomitans strain 624]
MQNLMAKGYLIHSQNHGDFTIAPELWLKNQQQQTVLLRYLALPLAENGQTLWLGLDSLTNLSACEAFSFLTGKTIEPVLI
ESQTLKTALQDLSPRVEKVEENQPLFYSALSQEQPTQPSDEPIIRLLNDIFESATAQKASDIHLEPQADCLQTRFRIDGV
LQVQNTVPLSIASRLISRLKLLAKLDISETRLPQDGRFQFKTTFSDTLDFRLSTLATQFGEKAVLRLQQNRPVQLAFGEL
GMTESQQQCFRRALSQPQGLILVTGPTGSGKSISLYTALQYLNTSEKHIMTAEDPIEIQLPGIIQSQVNPPIGLDFSRLL
RTFLRQDPDIIMLGEIRDEESAAMALRAAQTGHLVLSTLHTNDAPSAISRLQQLGIQRHEIDGSLLLVIAQRLVRKRCQK
CGGNATRFCDCHQGYKGRIGIYQFLQPNLQHPQGYETDFADLRQSALEKLKDGTTDLTEIQRVLGQAHD

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=158613 ACT75_RS07360 WP_005540457.1 1509981..1511390(-) (pilB) [Aggregatibacter actinomycetemcomitans strain 624]
ATGCAAAATCTGATGGCGAAAGGTTATTTGATTCACAGCCAAAATCACGGGGACTTTACCATTGCGCCCGAGCTATGGCT
TAAAAATCAGCAACAACAAACCGTCCTGTTGCGTTATCTCGCCCTGCCCTTAGCGGAAAACGGGCAGACCTTGTGGCTCG
GGTTAGACTCGTTAACTAACCTGAGCGCCTGTGAAGCCTTTTCTTTTCTCACCGGCAAAACCATTGAACCGGTATTAATT
GAAAGCCAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTA
TTCCGCACTGTCACAGGAACAGCCGACACAACCGTCTGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCG
CGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTG
CTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATAT
TAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCA
CCCTTGCCACCCAATTTGGTGAAAAAGCGGTCCTGCGTTTGCAACAAAATCGTCCCGTACAACTGGCATTCGGTGAATTG
GGTATGACGGAATCGCAGCAACAGTGTTTTCGTCGTGCCTTGAGCCAACCACAAGGCTTGATTTTAGTCACCGGTCCGAC
AGGCAGCGGCAAAAGTATTTCTTTATATACGGCGTTGCAGTACTTAAATACGTCTGAAAAACACATTATGACAGCGGAAG
ATCCCATTGAAATTCAACTCCCCGGCATTATTCAGAGCCAGGTGAATCCGCCTATCGGGTTGGATTTCAGCCGATTGCTT
CGCACCTTTTTACGCCAAGATCCGGACATTATTATGTTAGGTGAAATTCGTGATGAAGAAAGTGCCGCCATGGCGTTACG
CGCCGCGCAAACGGGGCATTTAGTGCTTTCGACGCTGCATACCAACGACGCCCCTTCCGCCATTTCCCGCCTGCAACAAC
TCGGCATTCAGCGCCATGAAATCGACGGCAGTTTGTTGCTGGTGATCGCCCAACGTTTGGTGCGCAAACGCTGCCAAAAG
TGCGGTGGAAATGCCACGCGTTTTTGCGATTGCCATCAAGGTTACAAAGGACGAATCGGGATCTATCAATTCCTGCAACC
GAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTACGTCAAAGCGCTTTAGAAAAACTCAAAGATGGCA
CCACAGATTTAACCGAAATCCAACGGGTATTAGGACAAGCCCATGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(31-93)

Type II/IV secretion system protein (T2SSE)

(130-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8KRY5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

70

100

0.701
  pilB Haemophilus influenzae 86-028NP

71.46

97.868

0.699
  pilB Glaesserella parasuis strain SC1401

57.328

98.934

0.567
  pilB Vibrio cholerae strain A1552

40.733

100

0.426
  pilB Acinetobacter baylyi ADP1

39.479

100

0.42
  pilB Vibrio parahaemolyticus RIMD 2210633

41.127

100

0.42
  pilB Vibrio campbellii strain DS40M4

40.166

100

0.414
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.412
  pilB Acinetobacter baumannii D1279779

39.264

100

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.03

100

0.394
  pilF Neisseria gonorrhoeae MS11

36

100

0.365

Multiple sequence alignment