Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACT75_RS07365 Genome accession   NZ_CP012959
Coordinates   1511417..1511869 (-) Length   150 a.a.
NCBI ID   WP_005550263.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain 624     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1506417..1516869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACT75_RS07340 (ACT75_07330) - 1506574..1507701 (-) 1128 WP_005539200.1 DUF4123 domain-containing protein -
  ACT75_RS07350 (ACT75_07340) - 1508079..1508768 (-) 690 WP_005594882.1 prepilin peptidase -
  ACT75_RS07355 (ACT75_07345) pilC 1508765..1509988 (-) 1224 WP_061866560.1 type II secretion system F family protein Machinery gene
  ACT75_RS07360 (ACT75_07350) pilB 1509981..1511390 (-) 1410 WP_005540457.1 GspE/PulE family protein Machinery gene
  ACT75_RS07365 (ACT75_07355) pilA 1511417..1511869 (-) 453 WP_005550263.1 pilin Machinery gene
  ACT75_RS07370 (ACT75_07360) ampD 1511991..1512545 (+) 555 WP_005540460.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACT75_RS07375 (ACT75_07365) nanQ 1513011..1513484 (-) 474 WP_005594879.1 N-acetylneuraminate anomerase -
  ACT75_RS07380 (ACT75_07370) pnp 1513778..1515907 (+) 2130 WP_032997160.1 polyribonucleotide nucleotidyltransferase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15503.95 Da        Isoelectric Point: 9.4349

>NTDB_id=158614 ACT75_RS07365 WP_005550263.1 1511417..1511869(-) (pilA) [Aggregatibacter actinomycetemcomitans strain 624]
MKIQSAFISLKFVKKGFTLIELMIVIAIVAILATVAVPSYQNYTKKAAVSELLQASAPLRSEVELCIYNTGKAENCSGGQ
NGIQADVADASKQKYVKSTAVKGGVITVTGKGSLDKISYTLTASGTAATGVSWNAACGNNAGIFPAGFCS

Nucleotide


Download         Length: 453 bp        

>NTDB_id=158614 ACT75_RS07365 WP_005550263.1 1511417..1511869(-) (pilA) [Aggregatibacter actinomycetemcomitans strain 624]
ATGAAAATTCAATCCGCGTTTATTTCGCTAAAATTCGTTAAAAAAGGGTTTACATTAATCGAATTAATGATCGTCATCGC
TATTGTTGCTATTCTCGCCACCGTAGCCGTACCATCTTATCAAAATTACACTAAAAAAGCGGCGGTTTCCGAATTATTAC
AAGCCTCCGCCCCATTACGTTCCGAAGTGGAACTCTGTATTTATAACACCGGTAAGGCTGAAAATTGTAGCGGCGGACAA
AACGGCATTCAAGCAGATGTTGCGGATGCTTCGAAACAAAAATATGTGAAATCCACTGCGGTCAAAGGCGGCGTCATCAC
CGTTACCGGCAAAGGCAGTTTGGATAAAATCAGCTACACCCTTACCGCTTCGGGCACGGCTGCCACCGGGGTCAGCTGGA
ACGCCGCCTGCGGCAATAATGCCGGCATTTTCCCGGCAGGTTTCTGTTCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(14-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.043

92

0.58
  pilA Haemophilus influenzae Rd KW20

59.028

96

0.567
  pilA Glaesserella parasuis strain SC1401

53.846

95.333

0.513

Multiple sequence alignment