Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AOC04_RS18660 Genome accession   NZ_CP012676
Coordinates   4174625..4175980 (-) Length   451 a.a.
NCBI ID   WP_060695980.1    Uniprot ID   -
Organism   Pseudomonas versuta strain L10.10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4169625..4180980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOC04_RS18645 (AOC04_18635) - 4171080..4172651 (-) 1572 WP_060695970.1 AAA family ATPase -
  AOC04_RS18650 (AOC04_18640) aroB 4172662..4173762 (-) 1101 WP_060695973.1 3-dehydroquinate synthase -
  AOC04_RS18655 (AOC04_18645) aroK 4174102..4174620 (-) 519 WP_060695977.1 shikimate kinase AroK -
  AOC04_RS18660 (AOC04_18650) pilQ 4174625..4175980 (-) 1356 WP_060695980.1 type IV pilus secretin PilQ Machinery gene
  AOC04_RS18665 (AOC04_18655) - 4175993..4176520 (-) 528 WP_060695982.1 pilus assembly protein PilP -
  AOC04_RS18670 (AOC04_18660) - 4176517..4177137 (-) 621 WP_060695985.1 type 4a pilus biogenesis protein PilO -
  AOC04_RS18675 (AOC04_18665) - 4177137..4177700 (-) 564 WP_060695987.1 PilN domain-containing protein -
  AOC04_RS18680 (AOC04_18670) pilM 4177700..4178665 (-) 966 WP_060695989.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 451 a.a.        Molecular weight: 48786.68 Da        Isoelectric Point: 4.6733

>NTDB_id=156243 AOC04_RS18660 WP_060695980.1 4174625..4175980(-) (pilQ) [Pseudomonas versuta strain L10.10]
MKRIFPVFGLALWSALNSPPILALAQAGALVAPGPEASGTVYAGEKISLNFQDIEVRTVLQLLADFTRFNLVVSDAVEGS
VTLTLQDVPWDQALELVLKSKDLDKRLEGNVLLVAPADEIDAREQHEQEVRNKLAEAVPLRRELVQVSHARAADIARLFE
SGAASQPGAEGRGSLMVDERTNHIIALQSPEQLEELRRIVAQLDVPVRQVMIEARIVEAGVDFERNLGVRWGGAINQGGK
WSAGGLEPAGGSGDTSFVDLGIRSNSSGLGIAFISNNVLLDLELSAMEKTGNGEIISQPKVVTADKETAKILKGTEIPYQ
ETSGSGATSVSFKEASLSLQVTPQITPDNSVIMEVVVTKDEPDYINMVNDVPPIKKNEVKAKVLVKDGETIVIGGVFSNT
QSNVIDKVPFFGDLPYVGRLFRRDVVQEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 1356 bp        

>NTDB_id=156243 AOC04_RS18660 WP_060695980.1 4174625..4175980(-) (pilQ) [Pseudomonas versuta strain L10.10]
ATGAAAAGGATTTTCCCGGTGTTTGGGCTGGCGCTATGGAGTGCGCTGAATTCGCCGCCAATACTGGCGCTCGCACAGGC
CGGTGCCCTTGTGGCACCAGGGCCCGAAGCGAGCGGTACGGTGTATGCGGGCGAAAAAATATCGCTTAACTTTCAGGACA
TCGAAGTGCGTACGGTGCTTCAGTTGCTGGCGGATTTCACCCGATTCAATCTGGTGGTCAGTGACGCCGTAGAGGGCAGT
GTGACGCTCACCCTGCAGGATGTTCCCTGGGATCAGGCACTGGAGCTGGTGCTCAAAAGTAAAGACCTCGACAAGCGCCT
TGAAGGCAATGTGCTGTTGGTTGCACCGGCTGACGAAATAGACGCCCGTGAACAGCATGAACAAGAAGTGCGAAATAAAC
TGGCCGAAGCTGTGCCTTTGCGTCGAGAGCTGGTGCAGGTCAGTCATGCCCGGGCAGCAGACATTGCACGGCTGTTCGAG
TCAGGGGCCGCTAGCCAGCCTGGCGCAGAAGGGCGGGGCTCGCTGATGGTCGATGAGCGTACCAATCACATCATTGCGCT
GCAGTCCCCCGAGCAGCTTGAAGAGTTGCGCCGCATCGTGGCTCAGCTGGATGTGCCGGTGCGTCAGGTAATGATCGAGG
CCCGTATTGTTGAGGCCGGGGTGGACTTTGAAAGGAACCTGGGGGTGCGCTGGGGCGGTGCGATCAACCAGGGTGGTAAG
TGGAGCGCTGGCGGTCTTGAACCGGCAGGTGGTAGCGGCGATACGAGCTTCGTCGATCTGGGTATCAGGAGTAATTCCTC
GGGGTTGGGCATCGCCTTCATCAGCAACAATGTGTTGCTTGACCTGGAGCTCAGCGCCATGGAAAAAACCGGGAATGGCG
AAATCATCTCTCAACCCAAGGTCGTTACCGCGGACAAGGAAACCGCCAAAATCCTTAAAGGCACCGAAATCCCCTATCAG
GAAACCAGTGGCAGCGGGGCAACCTCGGTGTCGTTCAAGGAAGCGTCGCTGTCGCTTCAAGTGACTCCGCAAATCACTCC
GGACAACAGCGTCATCATGGAAGTCGTTGTGACCAAGGACGAGCCTGATTACATCAATATGGTCAACGATGTGCCGCCGA
TCAAAAAGAATGAGGTCAAGGCCAAAGTGCTGGTTAAAGATGGTGAAACCATTGTGATTGGGGGGGTGTTCTCAAATACT
CAAAGCAATGTAATAGATAAAGTGCCATTTTTTGGCGATCTGCCGTATGTTGGCCGACTTTTCCGTAGAGACGTTGTCCA
GGAAAAAAAATCCGAACTGCTGGTGTTCCTGACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(286-442)

Bacterial type II/III secretion system short domain (Secretin_N)

(144-209)

Secretin and TonB N terminus short domain (STN)

(69-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

65.675

96.896

0.636
  comQ Acinetobacter baylyi ADP1

43.527

99.335

0.432
  pilQ Acinetobacter baumannii strain A118

41.489

100

0.432
  pilQ Acinetobacter baumannii D1279779

41.489

100

0.432
  pilQ Vibrio campbellii strain DS40M4

43.418

96.009

0.417
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

44.365

92.461

0.41
  pilQ Vibrio cholerae strain A1552

44.365

92.461

0.41
  pilQ Legionella pneumophila strain Lp02

43.705

93.348

0.408
  pilQ Legionella pneumophila strain ERS1305867

43.705

93.348

0.408

Multiple sequence alignment