Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AN401_RS16195 Genome accession   NZ_CP012621
Coordinates   3436759..3438474 (+) Length   571 a.a.
NCBI ID   WP_096779924.1    Uniprot ID   A0A291HSS3
Organism   Zobellella denitrificans strain F13-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3431759..3443474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AN401_RS16170 (AN401_15935) pdhR 3432556..3433317 (-) 762 WP_094041117.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AN401_RS16175 (AN401_15940) ampD 3433644..3434189 (-) 546 WP_096779922.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AN401_RS16180 (AN401_15945) - 3434273..3434782 (+) 510 WP_198401753.1 TIGR02281 family clan AA aspartic protease -
  AN401_RS16185 (AN401_15950) nadC 3434840..3435682 (+) 843 WP_096780394.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AN401_RS16190 (AN401_15955) - 3436303..3436746 (+) 444 WP_096779923.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AN401_RS16195 (AN401_15960) pilB 3436759..3438474 (+) 1716 WP_096779924.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AN401_RS16200 (AN401_15965) pilC 3438509..3439735 (+) 1227 WP_094041121.1 type II secretion system F family protein Machinery gene
  AN401_RS16205 (AN401_15970) pilD 3439793..3440647 (+) 855 WP_096779925.1 A24 family peptidase Machinery gene
  AN401_RS16210 (AN401_15975) coaE 3440644..3441249 (+) 606 WP_096779926.1 dephospho-CoA kinase -
  AN401_RS16215 (AN401_15980) trmB 3441325..3442041 (-) 717 WP_096779927.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  AN401_RS16220 (AN401_15985) - 3442195..3443373 (-) 1179 WP_094040173.1 PLP-dependent aminotransferase family protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62739.26 Da        Isoelectric Point: 5.6880

>NTDB_id=155793 AN401_RS16195 WP_096779924.1 3436759..3438474(+) (pilB) [Zobellella denitrificans strain F13-1]
MTMPINTIRSGLARSLAASSLLLTDELDQALALARQEGKPLSTVLVEQRLLSSAELARFCEQEYGLPLIDLNDFELDQVP
PQYLGMDLIERHHVIPIYLQGKVLYLAMSDPTNVAALEDFSFRFNLVTDALLVEEDKLQQMLVRLQQGGSSGVLDLEHIE
DSDIAGLVLDDSPVEQDDGLGRADEDAPIVRYINKIMLDAVRKEASDLHFEPYERFYRIRFRIDGMLHEVASPPVGLAAR
FAARLKVMARLDIAERRLPQDGRLKLRLTRNKSVDMRVNSLPTQWGEKIVIRILDSSGARLNIDQLGYDERQKQLYLSAL
RKPQGMILVTGPTGSGKTVSLYTGLSILNTVTANISTAEDPIEINLPGINQVQINPKAGLTFAHALRAFLRQDPDIIMVG
EIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLGNMGLPAYNIASSVSLIMAQRLARKLCPHCKVPEQVPEEQLRA
LGFTAGQQAQGVSLYRAVGCNRCTGGYKGRIGIYEVLPMSEKLAHLIMEGANSLTLAKAAEEDGMVSLHGSALEKARLGI
ISLAEVNRISG

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=155793 AN401_RS16195 WP_096779924.1 3436759..3438474(+) (pilB) [Zobellella denitrificans strain F13-1]
ATGACCATGCCTATCAATACCATTAGAAGCGGTCTGGCCAGAAGCCTGGCCGCTTCTTCGCTGTTGCTGACCGACGAACT
GGACCAGGCCCTGGCCCTGGCCCGGCAGGAGGGCAAGCCGCTCAGTACCGTGCTGGTGGAGCAGCGGCTGCTGTCCAGCG
CCGAGCTGGCGCGTTTTTGCGAGCAGGAATACGGCCTGCCGCTGATCGATCTCAACGATTTCGAGCTGGATCAGGTGCCG
CCCCAGTACCTGGGCATGGATCTTATCGAGCGCCATCACGTGATCCCCATCTACCTGCAGGGCAAGGTGCTCTACCTGGC
CATGTCCGATCCTACCAATGTGGCGGCGCTGGAAGACTTCAGCTTCCGCTTCAACCTGGTGACCGATGCCCTGCTGGTGG
AGGAAGACAAGCTGCAGCAGATGCTGGTCCGGCTGCAGCAGGGGGGCAGCAGCGGGGTGCTGGATCTCGAGCATATCGAA
GACTCCGATATCGCCGGCCTGGTGCTGGATGACTCCCCGGTGGAGCAGGACGATGGGCTGGGCCGCGCCGACGAGGACGC
CCCCATCGTCCGCTATATCAACAAGATCATGCTGGACGCGGTACGCAAGGAGGCCTCCGACCTGCATTTCGAGCCTTACG
AGCGCTTTTACCGCATCCGTTTTCGCATCGACGGCATGCTGCACGAGGTGGCCTCGCCGCCGGTCGGCCTGGCCGCCCGT
TTTGCCGCCCGGCTCAAGGTGATGGCCCGGCTGGACATCGCCGAGCGCCGCCTGCCCCAGGACGGCCGCCTCAAGCTCAG
GCTGACCCGCAACAAGTCGGTGGACATGAGGGTCAACAGCCTGCCCACCCAGTGGGGCGAGAAGATAGTCATCCGTATCC
TCGACAGCTCCGGCGCCCGGCTCAACATCGACCAGCTCGGCTACGACGAGCGGCAGAAACAGCTCTACCTGAGCGCCCTG
CGCAAACCCCAGGGCATGATACTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTCTCGCTGTACACCGGCCTGAGCAT
CCTCAATACCGTCACTGCCAACATTTCCACCGCCGAAGATCCCATAGAGATCAACCTGCCCGGCATCAACCAGGTGCAGA
TCAACCCCAAGGCCGGGCTGACCTTCGCCCACGCCCTGCGCGCCTTCCTGCGCCAGGATCCGGACATCATCATGGTGGGG
GAAATCCGCGATCTGGAAACCGCCGAAATCGCGGTCAAGGCCGCCCAGACCGGCCACCTGGTGCTGTCCACCCTGCACAC
CAACTCCGCCGCCGAAACCCTGACCCGGCTCGGCAACATGGGCCTGCCCGCCTACAATATCGCCTCGTCGGTATCCCTGA
TCATGGCCCAGCGGCTGGCGCGCAAGCTGTGCCCCCACTGCAAGGTGCCGGAACAGGTACCGGAAGAACAACTGCGGGCC
CTGGGCTTTACGGCCGGGCAGCAGGCCCAGGGGGTCAGCCTGTACAGGGCGGTGGGCTGCAACCGCTGCACCGGCGGCTA
CAAGGGGCGTATCGGCATCTACGAGGTGTTGCCGATGAGCGAGAAACTGGCCCACCTGATCATGGAGGGCGCCAACTCCC
TGACCCTGGCCAAGGCCGCCGAAGAAGACGGCATGGTCAGCCTGCACGGCTCCGCCCTGGAGAAGGCCCGGCTCGGCATC
ATCAGCCTGGCCGAGGTCAACCGCATCAGTGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A291HSS3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

53.974

94.746

0.511
  pilB Acinetobacter baylyi ADP1

51.51

98.599

0.508
  pilB Vibrio campbellii strain DS40M4

50.883

99.124

0.504
  pilB Acinetobacter baumannii D1279779

50.887

98.774

0.503
  pilB Vibrio cholerae strain A1552

53.445

94.046

0.503
  pilB Vibrio parahaemolyticus RIMD 2210633

50.619

98.949

0.501
  pilF Neisseria gonorrhoeae MS11

46.429

98.074

0.455
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.835

96.497

0.394
  pilB/pilB1 Synechocystis sp. PCC 6803

35.922

100

0.389
  pilF Thermus thermophilus HB27

37.696

100

0.378

Multiple sequence alignment