Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AMS64_RS03340 Genome accession   NZ_CP012504
Coordinates   692685..694391 (-) Length   568 a.a.
NCBI ID   WP_005336134.1    Uniprot ID   -
Organism   Aeromonas veronii strain TH0426     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 687685..699391
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMS64_RS03310 (AMS64_03290) mutT 688197..688601 (+) 405 WP_042053534.1 8-oxo-dGTP diphosphatase MutT -
  AMS64_RS03315 (AMS64_03295) yacG 688678..688872 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  AMS64_RS03320 (AMS64_03300) zapD 688882..689604 (-) 723 WP_005336141.1 cell division protein ZapD -
  AMS64_RS03325 (AMS64_03305) coaE 689642..690256 (-) 615 WP_064335312.1 dephospho-CoA kinase -
  AMS64_RS03330 (AMS64_03310) pilD 690270..691142 (-) 873 WP_064335313.1 A24 family peptidase Machinery gene
  AMS64_RS03335 (AMS64_03315) pilC 691204..692445 (-) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  AMS64_RS03340 (AMS64_03320) pilB 692685..694391 (-) 1707 WP_005336134.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  AMS64_RS03345 (AMS64_03325) tapA 694401..694826 (-) 426 WP_082182205.1 type IVa pilus major pilin TapA -
  AMS64_RS03350 (AMS64_03330) nadC 695154..696029 (-) 876 WP_064335315.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AMS64_RS03355 (AMS64_03335) - 696033..696503 (-) 471 WP_005336129.1 TIGR02281 family clan AA aspartic protease -
  AMS64_RS03360 (AMS64_03340) ampD 696634..697209 (+) 576 WP_064335316.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AMS64_RS03365 (AMS64_03345) pdhR 697488..698252 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62533.87 Da        Isoelectric Point: 5.3192

>NTDB_id=154825 AMS64_RS03340 WP_005336134.1 692685..694391(-) (pilB) [Aeromonas veronii strain TH0426]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=154825 AMS64_RS03340 WP_005336134.1 692685..694391(-) (pilB) [Aeromonas veronii strain TH0426]
ATGACCTCTAGCCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTTGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTCGGCTTCAGCTTCGGTCTTCATACTGAAGCCCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAACGACGCCCTCGGCATGGAGGATATCGACGAATCCGAGATT
TCGGATCTCGAGGTATCCGACGAAAACAGTCGGCTGGACGAAAGCGTCAATACCGCCGATGACGATGCTCCCATCGTCAA
GTACATCAACAAGATCCTGATGGACGCCATCAAGCGCGGTGCCTCCGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTCCGCTTTCGCATCGACGGCATTCTGCATGAAATCGCTACGCCGCCGGTCAACCTCTCCAACCGCTTCTCCGCCCGC
CTCAAGGTCATGGCGCGGCTCGATATTGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGCTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTTAATACCCTGCCCACCATGTGGGGGGAGAAGATTGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTTAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAGCCACAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAATATCTCTACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTTTTGCGGCAAGACCCGGATGTGGTGATGGTGGGTGAAATTCGCGAT
CTCGAAACCGCCGAAATCGCCATCAAGGCGGCACAGACCGGCCACCTGGTGCTATCCACCCTGCATACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAAGCGCCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGCTGGCAGCCGGACTGCGACTGTTCAAACCGGTCGGCTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATATACGAAATCATGCTGATGTCGGAAAACATCGCCAAATTAATCATGCAGGGGGCCAACTCCCTGCAAATCGCTG
CTATCGCGCAGAAAGAGGGGATGCGTACCTTACGTACCTCGGGACTGGAGAAGGCTCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377

Multiple sequence alignment