Detailed information NTG0154604

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: AMM49_RS05345
• Genome accession: NZ_CP012480
• Coordinates: 1044172..1045014 (-)
• Length: 280 a.a.
• NCBI ID: WP_001015250.1
• Uniprot ID: X5JZS7
• Organism: Streptococcus agalactiae strain NGBS128
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1039172..1050014

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AMM49_RS05335 (AMM49_05325)	-	1041119..1041820 (-)	702	WP_000120355.1	ABC transporter ATP-binding protein	-
AMM49_RS05340 (AMM49_05330)	topA	1041957..1044077 (-)	2121	WP_000246611.1	type I DNA topoisomerase	-
AMM49_RS05345 (AMM49_05335)	dprA	1044172..1045014 (-)	843	WP_001015250.1	DNA-processing protein DprA	Machinery gene
AMM49_RS05350 (AMM49_05340)	-	1045152..1046173 (-)	1022	Protein_968	siderophore ABC transporter substrate-binding protein	-
AMM49_RS05360 (AMM49_05350)	-	1046235..1046996 (-)	762	WP_000614758.1	ABC transporter ATP-binding protein	-
AMM49_RS05365 (AMM49_05355)	-	1046993..1047967 (-)	975	WP_000588583.1	iron chelate uptake ABC transporter family permease subunit	-
AMM49_RS05370 (AMM49_05360)	ceuB	1047964..1048926 (-)	963	WP_000735592.1	ABC transporter permease	Machinery gene
AMM49_RS05375 (AMM49_05365)	-	1049166..1049714 (-)	549	WP_000136503.1	sugar O-acetyltransferase	-

Sequence

Protein

• Length: 280 a.a.
• Molecular weight: 31271.32 Da
• Isoelectric Point: 9.3800

>NTDB_id=154604 AMM49_RS05345 WP_001015250.1 1044172..1045014(-) (dprA) [Streptococcus agalactiae strain NGBS128]
MNHFELFKLKKAGLTNLNINNIINYLKKNSLTSLSVRNMAVVSKCKNPTFFIENYKQLDLKKLRQEFKKFPVLSILDSNY
PLELKEIYNPPVLLFYQGNIELLSKPKLAVVGARQASQIGCQSVKKIIKETNNQFVIVSGLARGIDTAAHVSALKNGGSS
IAVIGSGLDVYYPTENKKLQEYMSYNHLVLSEYFTGEQPLKFHFPERNRIIAGLCQGIVVAEAKMRSGSLITCERALEEG
REVFAIPGNIIDGKSDGCHHLIQEGAKCIISGKDILSEYQ

Nucleotide

• Length: 843 bp

>NTDB_id=154604 AMM49_RS05345 WP_001015250.1 1044172..1045014(-) (dprA) [Streptococcus agalactiae strain NGBS128]
ATGAATCATTTTGAACTATTTAAGCTTAAAAAAGCTGGACTAACAAATCTTAATATCAACAATATTATCAACTATCTCAA
AAAGAATAGTTTAACTTCTCTATCTGTTCGCAATATGGCCGTAGTATCAAAATGTAAAAATCCTACTTTCTTTATAGAAA
ATTATAAACAGCTAGACCTTAAAAAACTTCGACAAGAATTCAAAAAATTTCCAGTTCTATCGATTTTGGATTCTAACTAT
CCTTTAGAGTTAAAAGAAATATATAATCCACCAGTTCTACTTTTTTATCAGGGTAATATTGAACTTCTATCTAAACCTAA
ATTAGCTGTAGTGGGGGCAAGACAGGCATCTCAGATAGGTTGTCAGTCTGTTAAAAAGATTATCAAGGAGACTAACAATC
AATTTGTTATCGTAAGTGGTTTAGCGCGTGGCATTGATACAGCAGCACATGTTAGTGCTTTAAAAAATGGCGGCAGTAGT
ATAGCTGTTATCGGGAGTGGTTTAGATGTTTATTATCCAACGGAGAATAAGAAACTTCAAGAATATATGTCATATAATCA
TCTCGTATTATCAGAATATTTTACCGGAGAACAACCCTTGAAATTTCATTTCCCCGAACGTAACCGTATTATTGCAGGGC
TGTGTCAAGGTATTGTGGTTGCCGAAGCTAAGATGAGATCTGGAAGTTTAATTACCTGTGAAAGAGCATTAGAAGAGGGA
CGAGAAGTTTTTGCCATTCCCGGAAATATTATCGATGGCAAATCAGATGGATGCCACCATCTTATCCAAGAAGGAGCTAA
ATGCATTATTTCAGGAAAAGATATCCTTTCTGAATATCAGTAG

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (69-270)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-63)

3D structure

• AlphaFold DB: X5JZS7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	70.251	99.643	0.7
dprA/cilB/dalA	Streptococcus mitis SK321	62.5	100	0.625
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	61.786	100	0.618
dprA/cilB/dalA	Streptococcus pneumoniae D39	61.786	100	0.618
dprA/cilB/dalA	Streptococcus pneumoniae R6	61.786	100	0.618
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	61.786	100	0.618
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	61.429	100	0.614
dprA	Lactococcus lactis subsp. cremoris KW2	61.29	99.643	0.611
dprA	Latilactobacillus sakei subsp. sakei 23K	42.231	89.643	0.379
dprA	Haemophilus influenzae Rd KW20	37.132	97.143	0.361