Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AK972_RS28940 Genome accession   NZ_CP012400
Coordinates   6118520..6119857 (-) Length   445 a.a.
NCBI ID   WP_063033466.1    Uniprot ID   -
Organism   Pseudomonas yamanorum strain LBUM636     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6113520..6124857
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AK972_RS28925 (AK972_5503) - 6115179..6116738 (-) 1560 WP_017478245.1 SPOR domain-containing protein -
  AK972_RS28930 (AK972_5504) aroB 6116749..6117849 (-) 1101 WP_003209346.1 3-dehydroquinate synthase -
  AK972_RS28935 (AK972_5505) aroK 6117997..6118515 (-) 519 WP_003209345.1 shikimate kinase AroK -
  AK972_RS28940 (AK972_5506) pilQ 6118520..6119857 (-) 1338 WP_063033466.1 type IV pilus secretin PilQ Machinery gene
  AK972_RS28945 (AK972_5507) - 6119927..6120940 (-) 1014 WP_063033467.1 pilus assembly protein PilP -
  AK972_RS28950 (AK972_5508) - 6120937..6121488 (-) 552 WP_063033468.1 PilN domain-containing protein -
  AK972_RS28955 (AK972_5509) pilM 6121488..6122141 (-) 654 WP_063033469.1 type IV pilus biogenesis protein PilM -
  AK972_RS28960 (AK972_5511) - 6122362..6124818 (+) 2457 WP_412458861.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 48546.58 Da        Isoelectric Point: 4.7160

>NTDB_id=154309 AK972_RS28940 WP_063033466.1 6118520..6119857(-) (pilQ) [Pseudomonas yamanorum strain LBUM636]
MVMAVPNRIDLIHLPPPGNAPLSTERVAFTGDKLSLNFQNIELRAVLQQIADVAGLNLVASDDVQGAITLRLKDVPWDQA
LDLVLQTKGLDKRVTANVLLVAPAEELAARELLVLESRKQMTELAPLRRELLQVNYAKASDLAKLFQSAVGLEGATDERG
SVAVDDRTNNIIAYQTQERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGQMSRGNWGAGGIRKPLAEGA
EPPENPPSSPFVDMGTVNGSAGLGIAFITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESSSTG
ATSVSFKEASLSLEVTPQITPDGWVNMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVQDGETIVIGGVFSNTQSKVVD
KVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=154309 AK972_RS28940 WP_063033466.1 6118520..6119857(-) (pilQ) [Pseudomonas yamanorum strain LBUM636]
ATGGTCATGGCTGTGCCCAATCGAATCGACCTGATTCACCTTCCGCCACCTGGCAATGCGCCCCTGAGTACCGAGCGCGT
GGCATTTACCGGCGACAAGCTGTCCCTCAACTTCCAGAACATCGAGTTACGTGCGGTGCTGCAGCAGATTGCCGACGTCG
CCGGCCTCAATCTGGTGGCCAGCGACGACGTGCAGGGGGCGATCACCCTGCGCCTCAAGGACGTGCCCTGGGACCAGGCC
CTGGATCTTGTGCTGCAAACCAAGGGGCTGGACAAACGTGTGACGGCCAACGTACTGCTGGTGGCGCCTGCCGAGGAGTT
GGCGGCCCGCGAGCTGCTGGTGCTGGAGTCGCGCAAGCAAATGACGGAGCTGGCCCCGTTGCGCCGTGAACTGCTGCAAG
TGAATTATGCCAAGGCGTCGGACCTGGCCAAGCTGTTCCAGTCGGCCGTGGGCCTGGAAGGTGCAACCGATGAGCGCGGC
TCGGTGGCGGTGGATGATCGCACCAACAACATCATCGCCTACCAGACCCAGGAGCGGCTGGAGGAGCTGCGTCGGATCGT
GGCGCAACTGGATATTCCGGTAAGGCAGGTGATGATCGAGGCGCGGATTGTCGAGGCCAATGTGGATTACGACAAAAGCC
TGGGGGTACGCTGGGGCGGGCAGATGAGTCGGGGTAACTGGGGCGCTGGCGGTATCAGGAAGCCGCTGGCCGAGGGCGCC
GAGCCTCCCGAAAACCCGCCCAGCTCGCCGTTTGTAGATATGGGCACGGTCAACGGCAGCGCCGGCCTGGGGATCGCCTT
TATCACCGACAACCTGCTGCTGGACCTGGAGCTGACCGCCATGGAAAAAACCGGCAACGGTGAAATCGTCTCGCAGCCCA
AGGTCGTCACCTCCGATAAGGAAACCGCACGAATCCTCAAGGGCACCGAAATTCCCTATCAGGAATCCAGCTCCACCGGT
GCTACCTCGGTGTCGTTCAAGGAAGCCTCGTTGTCACTGGAAGTGACGCCGCAAATCACACCGGACGGCTGGGTGAACAT
GGAGGTCAAGGTGACCAAGGACGAGCCCGACTACCTGAACAAGCTCAACGATGTGCCGCCGATCAAGAAAAACGAGGTCA
ATGCCAAGGTGCTGGTCCAGGACGGGGAGACCATCGTGATTGGCGGAGTTTTCTCCAATACTCAAAGCAAAGTGGTAGAT
AAAGTGCCATTTTTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGGGATGTGGTTTCGGAGAAAAAATCCGAGCT
GCTGGTATTCCTGACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(280-436)

Secretin and TonB N terminus short domain (STN)

(57-103)

Bacterial type II/III secretion system short domain (Secretin_N)

(131-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

69.69

100

0.708
  pilQ Vibrio campbellii strain DS40M4

45.863

95.056

0.436
  comQ Acinetobacter baylyi ADP1

43.111

100

0.436
  pilQ Acinetobacter baumannii strain A118

41.81

100

0.436
  pilQ Acinetobacter baumannii D1279779

41.81

100

0.436
  pilQ Legionella pneumophila strain ERS1305867

43.807

97.978

0.429
  pilQ Legionella pneumophila strain Lp02

43.807

97.978

0.429
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

45.215

93.933

0.425
  pilQ Vibrio cholerae strain A1552

45.215

93.933

0.425

Multiple sequence alignment