Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   DE8669_RS01860 Genome accession   NZ_CP012391
Coordinates   343616..344848 (-) Length   410 a.a.
NCBI ID   WP_002216405.1    Uniprot ID   Q7DDR1
Organism   Neisseria meningitidis strain DE8669     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 338616..349848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DE8669_RS01830 (DE8669_0336) - 339148..339594 (+) 447 WP_002220271.1 DUF441 domain-containing protein -
  DE8669_RS15155 - 339883..339945 (+) 63 Protein_330 transposase -
  DE8669_RS01840 (DE8669_0337) pilF 340085..341761 (+) 1677 WP_002221967.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DE8669_RS01845 (DE8669_0338) - 341846..342055 (-) 210 WP_002216401.1 DNA gyrase inhibitor YacG -
  DE8669_RS01850 (DE8669_0339) coaE 342048..342680 (-) 633 WP_002216402.1 dephospho-CoA kinase -
  DE8669_RS01855 (DE8669_0340) pilD 342682..343542 (-) 861 WP_002241067.1 prepilin peptidase Machinery gene
  DE8669_RS01860 (DE8669_0341) pilG 343616..344848 (-) 1233 WP_002216405.1 type 4 pilus assembly protein PilG Machinery gene
  DE8669_RS01865 (DE8669_0342) pgi 345358..347001 (+) 1644 WP_014581240.1 glucose-6-phosphate isomerase -
  DE8669_RS11765 - 346971..347201 (+) 231 WP_002212338.1 hypothetical protein -
  DE8669_RS01870 (DE8669_0343) dapD 347211..348032 (+) 822 WP_002255671.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  DE8669_RS01875 (DE8669_0344) fabI 348187..348972 (+) 786 WP_014581239.1 enoyl-ACP reductase FabI -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 45273.29 Da        Isoelectric Point: 9.9273

>NTDB_id=154061 DE8669_RS01860 WP_002216405.1 343616..344848(-) (pilG) [Neisseria meningitidis strain DE8669]
MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARKKLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLS
TMIKAGLPLMQAFEIVARGHGNPSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTVMDMSDFFVSYGWMVLIALGFAI
YGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTIARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRV
IQGLSMTSGMRATELFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIGTLLVAMYL
PLFNLGNVVA

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=154061 DE8669_RS01860 WP_002216405.1 343616..344848(-) (pilG) [Neisseria meningitidis strain DE8669]
ATGGCTAAAAACGGAGGATTTTCTTTGTTCGCAAAGAAAGAAAAACGCTTTATCTTTGAAGGCAGGCATTCCGCCTCCGA
CAAACTGGTCAACGGCGAAGTATCCGCGTTTACCGAAGAAGAGGCGCGCAAAAAACTGGCAAAACGCGGCATCCGCCCGT
TGCAGATTACCCGTGTGAAAACAAGCTCCAAGCGCAAAATCACACAAGAAGACATCACCGTTTTCACCCGCCAGCTGTCC
ACGATGATTAAAGCGGGCCTGCCGCTGATGCAGGCATTTGAAATCGTGGCGCGCGGACACGGCAACCCGTCTATGACGGA
AATGCTGATGGAAATCCGAGGCGAAGTGGAACAGGGCAGCTCGTTGAGCCGCGCATTCTCAAACCACCCGAAATATTTCG
ACCGCTTCTACTGCAACCTGGTTGCGGCAGGCGAAACGGGCGGCGTATTGGAAAGCCTGCTGGACAAATTGGCAATTTAC
AAAGAAAAAACCCAGGCCATCCGCAAAAAGGTAAAAACCGCACTGACCTATCCGGTATCCGTCATCGCCGTCGCCATCGG
TTTGGTATTCGTGATGATGATTTTCGTACTGCCCGCCTTTAAAGAAGTTTACGCCAATATGGGCGCGGAGCTTCCCGCAC
TGACCCAAACAGTGATGGATATGTCCGACTTTTTCGTCTCATACGGCTGGATGGTGCTGATCGCACTGGGCTTTGCCATA
TACGGCTTCCTTAAATTGAAGGCGCGTTCGATTAAAATCCAACGGCGTATGGATGCCATACTGCTGCGTATGCCGATTTT
CGGAGACATTGTCCGCAAAGGGACGATTGCCCGCTGGGGCAGGACGACAGCGACGCTGATTGCGGCAGGCGTGCCTTTGG
TCGATGTATTGGACTCCACTGCCGGCGCGGCGGGCAATTTAATCTATGAAGAAGCCACCCGGGAAATCCGTACGCGCGTC
ATCCAAGGTTTGTCTATGACTTCGGGGATGCGTGCGACGGAACTGTTCCCCAATATGATGTTGCAGATGTCTTCCATCGG
CGAGGAATCGGGTTCTTTGGACGATATGCTCAACAAAGCCGCCGAATTTTACGAAGACGAGGTGGACAATGCGGTCGGCA
GGCTGTCCGCTATGATGGAGCCGATCATTATCGTGATTTTAGGCTTGGTCATCGGCACGCTTCTGGTCGCCATGTATCTG
CCGCTGTTTAATTTGGGCAACGTGGTCGCCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(281-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q7DDR1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

99.268

100

0.993
  pilG Neisseria meningitidis 44/76-A

98.537

100

0.985
  pilC Acinetobacter baumannii D1279779

47.487

97.073

0.461
  pilC Acinetobacter baylyi ADP1

45.614

97.317

0.444
  pilC Pseudomonas stutzeri DSM 10701

44.913

98.293

0.441
  pilC Legionella pneumophila strain ERS1305867

44.836

96.829

0.434
  pilC Vibrio cholerae strain A1552

41.045

98.049

0.402
  pilC Vibrio campbellii strain DS40M4

39.163

99.024

0.388

Multiple sequence alignment