Detailed information NTG0154060

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: DE8669_RS01855
• Genome accession: NZ_CP012391
• Coordinates: 342682..343542 (-)
• Length: 286 a.a.
• NCBI ID: WP_002241067.1
• Uniprot ID: A1KVV2
• Organism: Neisseria meningitidis strain DE8669
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 337682..348542

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DE8669_RS01825 (DE8669_0335)	ispB	338169..339143 (+)	975	WP_002221965.1	octaprenyl diphosphate synthase	-
DE8669_RS01830 (DE8669_0336)	-	339148..339594 (+)	447	WP_002220271.1	DUF441 domain-containing protein	-
DE8669_RS15155	-	339883..339945 (+)	63	Protein_330	transposase	-
DE8669_RS01840 (DE8669_0337)	pilF	340085..341761 (+)	1677	WP_002221967.1	type IV-A pilus assembly ATPase PilB	Machinery gene
DE8669_RS01845 (DE8669_0338)	-	341846..342055 (-)	210	WP_002216401.1	DNA gyrase inhibitor YacG	-
DE8669_RS01850 (DE8669_0339)	coaE	342048..342680 (-)	633	WP_002216402.1	dephospho-CoA kinase	-
DE8669_RS01855 (DE8669_0340)	pilD	342682..343542 (-)	861	WP_002241067.1	prepilin peptidase	Machinery gene
DE8669_RS01860 (DE8669_0341)	pilG	343616..344848 (-)	1233	WP_002216405.1	type 4 pilus assembly protein PilG	Machinery gene
DE8669_RS01865 (DE8669_0342)	pgi	345358..347001 (+)	1644	WP_014581240.1	glucose-6-phosphate isomerase	-
DE8669_RS11765	-	346971..347201 (+)	231	WP_002212338.1	hypothetical protein	-
DE8669_RS01870 (DE8669_0343)	dapD	347211..348032 (+)	822	WP_002255671.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31331.48 Da
• Isoelectric Point: 9.1920

>NTDB_id=154060 DE8669_RS01855 WP_002241067.1 342682..343542(-) (pilD) [Neisseria meningitidis strain DE8669]
MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKKHLNLPLTEKESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL

Nucleotide

• Length: 861 bp

>NTDB_id=154060 DE8669_RS01855 WP_002241067.1 342682..343542(-) (pilD) [Neisseria meningitidis strain DE8669]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCGGTGTTCGGGCTGCTGGTCGGCAGTTTCTTAAA
CGTCGTCATTTACCGCGTGCCGGTCATGATGGAACGCGGCTGGACGGTATTTGCCAAAAAACATTTAAACCTGCCGCTGA
CCGAAAAGGAAAGCCGTACCTTCAACCTGATGAAACCGGATTCCTGCTGTCCCAAATGCCGCGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGCTACCTGCTCCTGCGCGGCAAATGCGCTTCCTGCCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTATTCGGGCTGGTCGCCTGGCAATACGGCTGGTCTTGGATTACATTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACACTGCCC
TTGATTTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATGGTTCGATATGGCTTTTATGCGCGGTATATAAACTGATTAAAGGCGAAATCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCGCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCAGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGCTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (134-242)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: A1KVV2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	96.14	99.65	0.958
pilD	Vibrio cholerae strain A1552	44.444	100	0.448
pilD	Acinetobacter baumannii D1279779	46.97	92.308	0.434
pilD	Acinetobacter nosocomialis M2	46.97	92.308	0.434
pilD	Vibrio campbellii strain DS40M4	44.565	96.503	0.43