Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AKN89_RS07990 Genome accession   NZ_CP012364
Coordinates   1678178..1679791 (+) Length   537 a.a.
NCBI ID   WP_414820346.1    Uniprot ID   -
Organism   Thiopseudomonas alkaliphila strain E1148     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1673178..1684791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AKN89_RS07970 (AKN89_07965) pilM 1675285..1676358 (+) 1074 WP_064503659.1 type IV pilus assembly protein PilM -
  AKN89_RS07975 (AKN89_07970) - 1676345..1676908 (+) 564 WP_053108377.1 PilN domain-containing protein -
  AKN89_RS07980 (AKN89_07975) - 1676920..1677531 (+) 612 WP_053108378.1 type 4a pilus biogenesis protein PilO -
  AKN89_RS07985 (AKN89_07980) - 1677528..1678058 (+) 531 WP_053108379.1 pilus assembly protein PilP -
  AKN89_RS07990 (AKN89_07985) pilQ 1678178..1679791 (+) 1614 WP_414820346.1 type IV pilus secretin PilQ Machinery gene
  AKN89_RS07995 (AKN89_07990) aroK 1679797..1680321 (+) 525 WP_080995523.1 shikimate kinase AroK -
  AKN89_RS08000 (AKN89_07995) aroB 1680372..1681484 (+) 1113 WP_053101910.1 3-dehydroquinate synthase -
  AKN89_RS08005 (AKN89_08000) - 1681488..1682663 (+) 1176 WP_053100533.1 SPOR domain-containing protein -
  AKN89_RS08010 (AKN89_08005) - 1682770..1683741 (+) 972 WP_053108381.1 phosphate ABC transporter substrate-binding protein PstS family protein -

Sequence

Protein


Download         Length: 537 a.a.        Molecular weight: 59573.16 Da        Isoelectric Point: 6.2989

>NTDB_id=153837 AKN89_RS07990 WP_414820346.1 1678178..1679791(+) (pilQ) [Thiopseudomonas alkaliphila strain E1148]
MLALQLAASELTTYSKVVSNTQVENLYFKRGGQGEGNVILTLTEPDVRFEVKEESDKIHLVLLNTSLSKQINTHLKTKEF
ATPVQLISMSQSGKDVSVIIELIDSYKYAVHQVGRQIIVSVNALPYKALEEHHSKNIRYVGEKISLNFQDVNLRAVLQVI
ADFTQLNLVVNDTVQGNITLQLQNVPWDQALDVILKTKGLAQRKEGNVLLIAPSEELAARERKELEIQRQAEELAPLRRE
LIQINYAKATDIAALFRLASGEQTEERGIITVDERTNAIIAYQTQERLAELSKIVSQLDTPVRQVMIEARIVEASVDYEK
ELGVRWGGERRGSSTHYNINNAFVDLGANNASSGVGIGFLSGKTRLDLELSAMEKTGNGEVVSQPKIITSDKETAKIMKG
SEVPYQEASSSGATSTSFKQAALSLEVTPYITPDNRILMEVRVTKDAPDFQKAINGVPPIDKNELSAKVLVVDGETIVIG
GVFQKVQKESLEKVPVLGDIPGLKGLFRRKMRRDDKTELLVFITPRIMNNQSISLNR

Nucleotide


Download         Length: 1614 bp        

>NTDB_id=153837 AKN89_RS07990 WP_414820346.1 1678178..1679791(+) (pilQ) [Thiopseudomonas alkaliphila strain E1148]
ATGTTGGCTCTACAGTTGGCGGCTTCAGAGTTAACAACCTATTCTAAAGTAGTCAGTAATACCCAGGTTGAAAATTTATA
TTTTAAGCGCGGTGGACAAGGAGAGGGTAATGTCATTTTGACCTTAACAGAACCTGATGTCCGATTTGAAGTAAAAGAAG
AGTCTGACAAGATTCACTTGGTTTTATTAAATACTAGCTTGTCCAAACAAATAAATACTCACTTAAAAACTAAAGAGTTT
GCAACACCTGTTCAACTGATTAGTATGAGTCAATCCGGAAAAGATGTATCAGTTATAATTGAGTTAATAGATAGCTATAA
GTACGCAGTGCATCAAGTAGGTAGGCAAATAATTGTGAGTGTAAACGCATTACCCTATAAAGCACTAGAGGAACATCATT
CGAAAAATATACGTTATGTGGGCGAAAAAATATCATTGAATTTTCAAGATGTTAATCTGAGAGCGGTACTACAAGTTATT
GCTGACTTTACTCAGCTTAATCTGGTGGTTAATGATACAGTGCAAGGCAATATTACATTGCAATTACAAAATGTGCCTTG
GGATCAAGCGCTGGATGTAATATTAAAAACTAAAGGGCTTGCTCAGCGTAAAGAGGGAAACGTGTTGTTGATTGCGCCCT
CTGAGGAACTGGCAGCACGTGAGCGTAAGGAGTTGGAAATACAGCGTCAAGCTGAAGAACTAGCCCCATTACGTCGTGAG
TTAATTCAAATTAACTATGCTAAAGCTACCGATATTGCCGCGTTATTCCGGTTGGCCTCAGGGGAGCAAACAGAAGAACG
CGGAATTATTACTGTGGATGAGCGGACCAATGCAATTATTGCTTACCAAACACAAGAGCGTTTAGCAGAGCTTAGTAAAA
TTGTCTCTCAGCTAGATACACCAGTACGTCAAGTAATGATTGAGGCGCGAATTGTTGAAGCTAGCGTGGACTATGAAAAA
GAACTTGGAGTTAGATGGGGAGGAGAGCGTAGAGGGAGCAGTACGCATTATAATATTAACAATGCTTTTGTAGACTTGGG
TGCCAATAATGCGAGCTCTGGAGTAGGCATCGGTTTTCTCAGTGGTAAAACTCGATTAGATCTAGAATTGTCAGCAATGG
AGAAAACTGGCAATGGGGAAGTTGTTTCGCAGCCCAAAATTATTACATCAGATAAAGAAACAGCGAAAATCATGAAGGGC
TCGGAAGTGCCTTACCAAGAGGCCAGCTCCAGTGGCGCTACCAGTACTTCATTTAAACAGGCAGCTCTATCGTTAGAGGT
TACACCCTACATTACGCCTGATAATCGTATTTTGATGGAAGTAAGAGTGACTAAAGACGCGCCAGATTTTCAAAAGGCTA
TTAATGGTGTTCCGCCGATTGATAAGAATGAGTTAAGTGCTAAGGTGTTAGTGGTGGATGGTGAAACTATCGTAATTGGT
GGCGTGTTTCAAAAAGTACAAAAAGAGTCCTTAGAAAAAGTGCCAGTGTTAGGAGATATCCCAGGCCTTAAAGGATTGTT
CCGCCGTAAGATGAGGCGAGATGATAAAACTGAATTATTAGTTTTCATTACGCCAAGAATCATGAATAATCAAAGTATTT
CCTTGAATCGTTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(166-213)

Bacterial type II and III secretion system protein (Secretin)

(372-528)

Bacterial type II/III secretion system short domain (Secretin_N)

(241-304)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.423

100

0.607
  pilQ Acinetobacter baumannii D1279779

39.652

100

0.425
  pilQ Acinetobacter baumannii strain A118

39.652

100

0.425
  comQ Acinetobacter baylyi ADP1

39.572

100

0.413
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.241

100

0.397
  pilQ Vibrio cholerae strain A1552

38.241

100

0.397
  pilQ Legionella pneumophila strain ERS1305867

40.655

96.648

0.393
  pilQ Legionella pneumophila strain Lp02

40.462

96.648

0.391
  pilQ Vibrio campbellii strain DS40M4

38.069

100

0.389

Multiple sequence alignment