Detailed information NTG0153817

Bioinformatically predicted

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: AKN90_RS07975
• Genome accession: NZ_CP012363
• Coordinates: 1706479..1708092 (+)
• Length: 537 a.a.
• NCBI ID: WP_414820312.1
• Uniprot ID: -
• Organism: Thiopseudomonas alkaliphila strain E1086
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1684038..1706359	1706479..1708092	flank	120

Gene organization within MGE regions

Location: 1684038..1708092

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AKN90_RS07905 (AKN90_07920)	-	1684038..1685258 (+)	1221	WP_053102924.1	HlyD family type I secretion periplasmic adaptor subunit	-
AKN90_RS07910	-	1685960..1689937 (+)	3978	WP_053106256.1	Mur ligase family protein	-
AKN90_RS07915 (AKN90_07930)	-	1690067..1691416 (+)	1350	WP_199532920.1	TolC family outer membrane protein	-
AKN90_RS07920 (AKN90_07935)	-	1691413..1693590 (+)	2178	WP_231692597.1	type I secretion system permease/ATPase	-
AKN90_RS07925 (AKN90_07940)	-	1693612..1694832 (+)	1221	WP_053102924.1	HlyD family type I secretion periplasmic adaptor subunit	-
AKN90_RS07930	-	1694906..1696240 (+)	1335	WP_053106257.1	glycosyltransferase family 2 protein	-
AKN90_RS07935 (AKN90_07950)	ppk1	1696646..1698775 (-)	2130	Protein_1564	polyphosphate kinase 1	-
AKN90_RS07940 (AKN90_07955)	rpmE	1699081..1699293 (+)	213	WP_053100542.1	50S ribosomal protein L31	-
AKN90_RS07945 (AKN90_07960)	-	1699743..1701011 (+)	1269	WP_053100541.1	malic enzyme-like NAD(P)-binding protein	-
AKN90_RS07950 (AKN90_07965)	-	1701091..1703271 (-)	2181	WP_053106258.1	penicillin-binding protein 1A	-
AKN90_RS07955 (AKN90_07970)	pilM	1703586..1704659 (+)	1074	WP_158487769.1	type IV pilus assembly protein PilM	-
AKN90_RS07960 (AKN90_07975)	-	1704646..1705209 (+)	564	WP_053100538.1	PilN domain-containing protein	-
AKN90_RS07965 (AKN90_07980)	-	1705221..1705832 (+)	612	WP_053106260.1	type 4a pilus biogenesis protein PilO	-
AKN90_RS07970 (AKN90_07985)	-	1705829..1706359 (+)	531	WP_053104862.1	pilus assembly protein PilP	-
AKN90_RS07975 (AKN90_07990)	pilQ	1706479..1708092 (+)	1614	WP_414820312.1	type IV pilus secretin PilQ	Machinery gene

Sequence

Protein

• Length: 537 a.a.
• Molecular weight: 59479.99 Da
• Isoelectric Point: 6.4016

>NTDB_id=153817 AKN90_RS07975 WP_414820312.1 1706479..1708092(+) (pilQ) [Thiopseudomonas alkaliphila strain E1086]
MLALQLAASELTTYSKVVSNTQIENLYFKRGGQGEGNVILTLTEPDVRFEVKEESDKIHLVLLNTSLSKQTSTRLKAKGF
ATPVQLISMSQSGKDVSVIIELIDNYKYAVHQVGRQITVSVNALPYKALEEHHSKNIRYVGEKISLNFQDVNLRAVLQVI
ADFTQLNLVVNDTVQGNITLQLQNVPWDQALDVILKTKGLAQRKEGNVLLIAPSEELAARERQELEIQRQAEELAPLRRE
LIQINYAKATDIAALFRLASGEQTEERGIITVDERTNAIIAYQTQERLAELSKIVSQLDTPVRQVMIEARIVEASVDYEK
ELGVRWGGERRGSSTHYNINNAFVDLGANNASSGVGIGFLSGKTRLDLELSAMEKTGNGEVVSQPKIITSDKETAKIMKG
SEVPYQEASSSGATSTSFKQAALSLEVTPYITPDNRILMEVRVTKDAPDFQKAINGVPPIDKNELSAKVLVVDGETIVIG
GVFQKVQKESLEKVPVLGDIPGLKGLFRRKMRRDDKTELLVFITPRIMNNQSISLNR

Nucleotide

• Length: 1614 bp

>NTDB_id=153817 AKN90_RS07975 WP_414820312.1 1706479..1708092(+) (pilQ) [Thiopseudomonas alkaliphila strain E1086]
ATGTTGGCTCTACAATTGGCGGCTTCAGAGTTAACAACCTATTCTAAAGTAGTCAGTAATACCCAGATTGAAAATTTATA
TTTTAAGCGCGGTGGACAAGGAGAGGGTAATGTTATCTTGACCTTAACAGAACCTGATGTCCGATTTGAAGTAAAAGAAG
AGTCTGACAAGATTCACTTAGTTTTATTAAATACTAGCTTGTCCAAACAAACAAGTACTCGCTTAAAAGCTAAGGGGTTT
GCAACACCTGTTCAACTGATTAGTATGAGTCAATCAGGAAAAGATGTATCAGTTATAATTGAGTTAATAGATAACTATAA
GTACGCAGTGCATCAAGTAGGTAGGCAAATAACTGTGAGTGTAAACGCATTACCCTATAAAGCACTAGAGGAACATCACT
CGAAAAATATACGTTATGTGGGCGAAAAGATATCATTGAATTTTCAAGATGTTAATCTGAGAGCGGTACTACAAGTTATT
GCTGACTTTACTCAGCTTAATCTGGTGGTTAATGATACAGTGCAAGGCAATATTACATTGCAATTACAAAATGTGCCTTG
GGATCAAGCGCTGGATGTAATATTAAAAACTAAAGGGCTTGCTCAGCGTAAAGAGGGAAACGTGTTGTTGATTGCGCCCT
CTGAGGAACTGGCAGCACGTGAGCGTCAGGAGTTGGAAATACAGCGTCAAGCTGAAGAACTAGCCCCATTACGTCGTGAG
TTAATTCAAATTAACTATGCTAAAGCTACCGATATTGCCGCGTTATTCCGGTTGGCCTCAGGGGAGCAAACAGAAGAACG
CGGAATTATTACTGTGGATGAGCGGACCAATGCAATTATTGCTTACCAAACACAAGAGCGTTTAGCAGAGCTTAGTAAAA
TTGTCTCTCAGCTAGATACACCAGTACGTCAAGTAATGATTGAGGCGCGAATTGTTGAAGCTAGCGTGGACTATGAAAAA
GAACTTGGAGTTAGATGGGGAGGAGAGCGTAGAGGGAGCAGTACGCATTATAATATTAACAATGCTTTTGTAGACTTGGG
TGCCAATAATGCGAGCTCTGGAGTAGGCATTGGTTTTCTCAGTGGTAAAACTCGATTAGATCTAGAATTGTCAGCAATGG
AGAAAACTGGCAATGGGGAAGTTGTTTCGCAGCCCAAAATTATTACATCAGATAAAGAAACAGCGAAAATCATGAAGGGC
TCGGAAGTGCCTTACCAAGAGGCCAGCTCCAGTGGCGCTACCAGTACTTCATTTAAACAGGCAGCTCTATCGTTAGAGGT
TACACCCTACATTACGCCTGATAATCGTATTTTGATGGAAGTAAGAGTGACTAAAGACGCGCCAGATTTTCAAAAGGCTA
TTAATGGTGTTCCGCCGATTGATAAGAATGAGTTAAGTGCTAAGGTGTTAGTGGTGGATGGTGAAACTATCGTAATTGGT
GGCGTGTTTCAAAAAGTACAAAAAGAGTCCTTAGAAAAAGTGCCAGTGTTAGGAGATATCCCAGGCCTTAAAGGATTGTT
CCGCCGTAAGATGAGGCGAGATGATAAAACTGAATTATTAGTTTTCATTACGCCAAGAATCATGAATAATCAAAGTATTT
CCTTGAATCGTTAA

Domains

Predicted by InterproScan.

• Secretin and TonB N terminus short domain (STN) (166-213)

• Bacterial type II and III secretion system protein (Secretin) (372-528)

• Bacterial type II/III secretion system short domain (Secretin_N) (241-304)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Pseudomonas aeruginosa PAK	59.14	100	0.615
pilQ	Acinetobacter baumannii D1279779	39.652	100	0.425
pilQ	Acinetobacter baumannii strain A118	39.652	100	0.425
comQ	Acinetobacter baylyi ADP1	39.75	100	0.415
pilQ	Legionella pneumophila strain ERS1305867	40.15	99.255	0.399
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	38.42	100	0.399
pilQ	Vibrio cholerae strain A1552	38.42	100	0.399
pilQ	Legionella pneumophila strain Lp02	39.962	99.255	0.397
pilQ	Vibrio campbellii strain DS40M4	38.251	100	0.391