Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AKN91_RS06605 Genome accession   NZ_CP012362
Coordinates   1388468..1390141 (-) Length   557 a.a.
NCBI ID   WP_053104861.1    Uniprot ID   -
Organism   Thiopseudomonas alkaliphila strain D3318     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1383468..1395141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AKN91_RS06585 (AKN91_06585) - 1384518..1385489 (-) 972 WP_053100532.1 phosphate ABC transporter substrate-binding protein PstS family protein -
  AKN91_RS06590 (AKN91_06590) - 1385596..1386771 (-) 1176 WP_053100533.1 SPOR domain-containing protein -
  AKN91_RS06595 (AKN91_06595) aroB 1386775..1387887 (-) 1113 WP_053101910.1 3-dehydroquinate synthase -
  AKN91_RS06600 (AKN91_06600) aroK 1387938..1388462 (-) 525 WP_080995523.1 shikimate kinase AroK -
  AKN91_RS06605 (AKN91_06605) pilQ 1388468..1390141 (-) 1674 WP_053104861.1 type IV pilus secretin PilQ Machinery gene
  AKN91_RS06610 (AKN91_06610) - 1390201..1390731 (-) 531 WP_053104862.1 pilus assembly protein PilP -
  AKN91_RS10980 (AKN91_06615) pilO 1390728..1391339 (-) 612 WP_064502523.1 type 4a pilus biogenesis protein PilO -
  AKN91_RS06620 (AKN91_06620) - 1391351..1391914 (-) 564 WP_053109667.1 PilN domain-containing protein -
  AKN91_RS06625 (AKN91_06625) pilM 1391901..1392974 (-) 1074 WP_064502524.1 type IV pilus assembly protein PilM -

Sequence

Protein


Download         Length: 557 a.a.        Molecular weight: 61955.12 Da        Isoelectric Point: 6.0665

>NTDB_id=153790 AKN91_RS06605 WP_053104861.1 1388468..1390141(-) (pilQ) [Thiopseudomonas alkaliphila strain D3318]
MVANFFQRFVMLLVIILFDAMLALQLAASELTIYSKVVSNTQVENLYFKRGGQGEGNVILTLTEPDVRFEVKEESDKIHL
VLLNTSLSKQINTHLKTKEFATPVQLISMSQSGKDVSVIIELIDSYKYAVHQVGRQIIVSVNALPYKALEEHHSKNIRYV
GEKISLNFQDVNLRAVLQVIADFTQLNLVVNDTVQGNITLQLQNVPWDQALDVILKTKGLAQRKEGNVLLIAPSEELAAR
ERQELEIQRQAEELAPLRRELIQINYAKATDIAALFRLASGEQTEERGIITVDERTNAIIAYQTQERLAELSKIVSQLDT
PVRQVMIEARIVEASVDYEKELGVRWGGERRGSSTHYNINNAFVDLGANNASSGVGIGFLSGKTRLDLELSAMEKTGNGE
VVSQPKIITSDKETAKIMKGSEVPYQEASSSGATSTSFKQAALSLEVTPYITPDNRILMEVRVTKDAPDFQKAINGVPPI
DKNELSAKVLVVDGETIVIGGVFQKVQKESLEKVPVLGDIPGLKGLFRRKMRRDDKTELLVFITPRIMNNQSISLNR

Nucleotide


Download         Length: 1674 bp        

>NTDB_id=153790 AKN91_RS06605 WP_053104861.1 1388468..1390141(-) (pilQ) [Thiopseudomonas alkaliphila strain D3318]
ATGGTAGCTAATTTTTTTCAGCGATTTGTTATGCTGTTAGTAATTATACTTTTCGATGCAATGTTGGCTCTACAGTTGGC
GGCTTCAGAGTTAACAATCTATTCTAAAGTAGTCAGTAATACTCAGGTTGAAAATTTATATTTTAAGCGCGGTGGACAAG
GAGAGGGTAATGTCATTTTGACCTTAACAGAACCTGATGTCCGATTTGAAGTAAAAGAAGAGTCTGACAAGATTCACTTG
GTTTTATTAAATACTAGCTTGTCCAAACAAATAAATACTCACTTAAAAACTAAAGAGTTTGCAACACCTGTTCAACTGAT
TAGTATGAGTCAATCCGGAAAAGATGTATCAGTTATAATTGAGTTAATAGATAGCTATAAGTACGCAGTGCATCAAGTAG
GTAGGCAAATAATTGTGAGTGTAAACGCATTACCCTATAAAGCACTAGAGGAACATCATTCGAAAAATATACGTTATGTA
GGCGAAAAGATATCATTGAATTTTCAAGATGTTAATCTGAGAGCGGTACTACAAGTTATTGCTGACTTTACTCAGCTTAA
TCTGGTGGTTAATGATACAGTGCAAGGCAATATTACATTGCAATTACAAAATGTGCCTTGGGATCAAGCGCTGGATGTAA
TATTAAAAACTAAAGGGCTTGCTCAGCGTAAAGAGGGAAACGTGTTGTTGATTGCGCCCTCTGAGGAACTGGCAGCACGT
GAGCGTCAGGAGTTGGAAATACAGCGTCAAGCTGAAGAACTAGCCCCATTACGTCGTGAGTTAATTCAAATTAACTATGC
TAAAGCTACCGATATTGCCGCGTTATTCCGGTTGGCCTCAGGGGAGCAAACAGAAGAACGCGGAATTATTACCGTGGATG
AGCGGACCAATGCAATTATTGCTTACCAAACACAAGAGCGTTTAGCAGAGCTTAGTAAAATTGTCTCTCAGCTAGATACA
CCAGTACGTCAAGTAATGATTGAGGCGCGAATTGTTGAAGCTAGCGTGGACTATGAAAAAGAACTTGGAGTTAGATGGGG
AGGAGAGCGTAGAGGGAGCAGTACGCATTATAATATTAACAATGCTTTTGTAGACTTGGGTGCCAATAATGCGAGCTCTG
GAGTAGGCATCGGTTTTCTCAGTGGTAAAACTCGATTAGATCTAGAATTGTCAGCAATGGAGAAAACTGGCAATGGGGAA
GTTGTTTCGCAGCCCAAAATTATTACATCAGATAAAGAAACAGCGAAAATCATGAAGGGCTCGGAAGTGCCTTACCAAGA
GGCCAGCTCCAGTGGCGCTACCAGTACTTCATTTAAACAGGCAGCTCTATCGTTAGAGGTTACACCCTACATTACGCCTG
ATAATCGTATTTTGATGGAAGTAAGAGTGACTAAAGACGCGCCAGATTTTCAAAAGGCTATTAATGGTGTTCCGCCGATT
GATAAGAATGAGTTAAGTGCTAAGGTGTTAGTGGTGGATGGTGAAACTATCGTAATTGGTGGCGTGTTTCAAAAAGTACA
AAAAGAGTCCTTAGAAAAAGTGCCAGTGTTAGGAGATATCCCAGGCCTTAAAGGATTGTTCCGCCGTAAAATGAGGCGAG
ATGATAAAACTGAATTATTAGTTTTCATTACGCCAAGAATCATGAATAATCAAAGTATTTCCTTGAATCGTTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(186-233)

Bacterial type II/III secretion system short domain (Secretin_N)

(261-324)

Bacterial type II and III secretion system protein (Secretin)

(392-548)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.602

100

0.587
  pilQ Acinetobacter baumannii D1279779

39.652

100

0.409
  pilQ Acinetobacter baumannii strain A118

39.652

100

0.409
  comQ Acinetobacter baylyi ADP1

39.572

100

0.399
  pilQ Legionella pneumophila strain ERS1305867

39.416

98.384

0.388
  pilQ Legionella pneumophila strain Lp02

39.234

98.384

0.386
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.42

100

0.384
  pilQ Vibrio cholerae strain A1552

38.42

100

0.384
  pilQ Vibrio campbellii strain DS40M4

38.251

98.564

0.377

Multiple sequence alignment