Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AKN87_RS10150 Genome accession   NZ_CP012358
Coordinates   2087271..2088488 (+) Length   405 a.a.
NCBI ID   WP_053103342.1    Uniprot ID   -
Organism   Thiopseudomonas alkaliphila strain B4199     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2082271..2093488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AKN87_RS10125 (AKN87_10130) rplQ 2083149..2083532 (+) 384 WP_053100999.1 50S ribosomal protein L17 -
  AKN87_RS10130 (AKN87_10135) - 2083611..2084027 (+) 417 WP_053103338.1 PACE efflux transporter -
  AKN87_RS10135 (AKN87_10140) elbB 2084070..2084723 (+) 654 WP_053103339.1 isoprenoid biosynthesis glyoxalase ElbB -
  AKN87_RS10140 (AKN87_10145) - 2084794..2085303 (-) 510 WP_053103340.1 pilin -
  AKN87_RS10145 (AKN87_10150) pilB 2085574..2087265 (+) 1692 WP_053103341.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AKN87_RS10150 (AKN87_10155) pilC 2087271..2088488 (+) 1218 WP_053103342.1 type II secretion system F family protein Machinery gene
  AKN87_RS10155 (AKN87_10160) pilD 2088492..2089388 (+) 897 WP_053103343.1 A24 family peptidase Machinery gene
  AKN87_RS10160 (AKN87_10165) - 2089820..2090809 (-) 990 WP_053103344.1 hypothetical protein -
  AKN87_RS10165 (AKN87_10170) - 2090980..2092191 (-) 1212 WP_053103345.1 NAD(P)/FAD-dependent oxidoreductase -
  AKN87_RS10170 (AKN87_10175) - 2092188..2092646 (-) 459 WP_053103346.1 YiiD C-terminal domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44054.76 Da        Isoelectric Point: 9.7260

>NTDB_id=153741 AKN87_RS10150 WP_053103342.1 2087271..2088488(+) (pilC) [Thiopseudomonas alkaliphila strain B4199]
MEIKKSPKSYMYVWHGKSRQGNKLSGVINSHDINIVKAQLRKQGVLHAKVKKKGITFGRDKKIKSMDITVFTRQMATMMQ
AGVPLLQSFTIIIEGLDNSNMRKLLEAVRNDIAGGSSFAGALRKHSLYFDELYCNLVASGETSGALETLLDRVATYKEKS
EALKSKIKKAMNYPIAVVVVAAVVTSILLLKVVPQFQEIFEGFGAELPVFTQLVIGISELLQEYSLYVLIGLVLLLYAAR
RTLRLSEQLRNRADKILLKMPIIGDILYNSAVARYGRTLATTFSAGVPLVDALDSVSGATGNVVFKNAVQQVKADVSTGV
QLHTSMMSSGVFPPLALQLTAIGEESGALDTMLEKVATHYEAEVDNAVDGLTALLEPIIMSVLGVLIGGLIIAMYLPIFQ
MGNAV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=153741 AKN87_RS10150 WP_053103342.1 2087271..2088488(+) (pilC) [Thiopseudomonas alkaliphila strain B4199]
ATGGAAATTAAGAAGTCGCCTAAAAGCTATATGTATGTTTGGCATGGTAAAAGCAGGCAAGGGAATAAGTTAAGTGGTGT
TATTAATTCGCATGATATTAATATTGTAAAGGCTCAGCTAAGAAAGCAGGGAGTTCTGCATGCTAAGGTGAAAAAGAAAG
GTATCACCTTTGGTCGCGATAAAAAAATTAAATCCATGGACATAACCGTCTTTACTCGGCAAATGGCCACCATGATGCAA
GCCGGGGTGCCTTTATTACAGTCGTTTACCATTATTATTGAAGGCTTAGACAATTCCAATATGCGTAAATTACTAGAAGC
TGTGCGTAATGATATTGCTGGGGGTAGCAGTTTTGCAGGAGCTTTACGCAAACACTCACTATATTTTGATGAGTTGTACT
GCAATTTAGTGGCCTCAGGCGAAACGTCGGGAGCTTTGGAAACTTTGCTTGATCGAGTAGCGACCTATAAAGAAAAGTCA
GAGGCGCTTAAATCTAAAATTAAAAAAGCCATGAACTACCCGATTGCTGTAGTGGTGGTGGCAGCTGTTGTGACGAGTAT
TTTGCTATTAAAAGTGGTACCACAGTTCCAGGAGATCTTTGAAGGTTTTGGGGCTGAATTGCCTGTATTTACGCAATTGG
TAATTGGTATTTCAGAGTTGCTGCAAGAGTATAGTTTATATGTGCTGATAGGCTTAGTACTGTTACTTTATGCTGCTCGT
AGGACGTTGCGCCTATCAGAGCAGTTGCGCAATCGTGCGGATAAAATACTGCTAAAAATGCCGATAATAGGTGATATTTT
ATATAACTCTGCTGTGGCTCGTTATGGGCGCACCTTAGCTACAACCTTCTCAGCCGGCGTACCTTTGGTTGATGCGTTAG
ATTCAGTTTCTGGGGCAACGGGTAACGTGGTTTTTAAAAACGCAGTGCAGCAAGTAAAAGCCGATGTATCTACCGGTGTG
CAGTTACACACTTCAATGATGTCGAGCGGAGTATTTCCTCCTTTAGCACTGCAACTGACTGCCATTGGTGAAGAGTCAGG
TGCGCTAGATACCATGCTAGAAAAAGTTGCCACTCACTATGAAGCCGAGGTAGATAACGCGGTAGATGGTTTAACTGCGT
TACTAGAGCCAATCATTATGTCAGTGCTAGGAGTATTAATTGGTGGGCTAATTATTGCCATGTACCTGCCAATCTTCCAG
ATGGGTAACGCGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(277-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.012

99.506

0.647
  pilC Acinetobacter baumannii D1279779

54.412

100

0.548
  pilC Acinetobacter baylyi ADP1

53.641

100

0.546
  pilC Legionella pneumophila strain ERS1305867

49.878

100

0.504
  pilG Neisseria meningitidis 44/76-A

42.607

98.519

0.42
  pilG Neisseria gonorrhoeae MS11

42.607

98.519

0.42
  pilC Vibrio campbellii strain DS40M4

40.955

98.272

0.402
  pilC Vibrio cholerae strain A1552

40.816

96.79

0.395
  pilC Thermus thermophilus HB27

39.599

98.519

0.39

Multiple sequence alignment