Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   P40_RS16570 Genome accession   NZ_CP012331
Coordinates   3595252..3596964 (+) Length   570 a.a.
NCBI ID   WP_080531404.1    Uniprot ID   -
Organism   Alloalcanivorax xenomutans strain P40     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3590252..3601964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P40_RS16550 (P40_16345) - 3590731..3591225 (+) 495 WP_080531400.1 DUF1850 domain-containing protein -
  P40_RS16555 (P40_16350) - 3591222..3593432 (+) 2211 WP_080531401.1 TRAP transporter permease -
  P40_RS22145 - 3593794..3594222 (+) 429 WP_145953609.1 hypothetical protein -
  P40_RS16565 (P40_16360) - 3594420..3594890 (-) 471 WP_080531403.1 pilin -
  P40_RS16570 (P40_16365) pilB 3595252..3596964 (+) 1713 WP_080531404.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P40_RS16575 (P40_16370) pilC 3597035..3598258 (+) 1224 WP_022995251.1 type II secretion system F family protein Machinery gene
  P40_RS16580 (P40_16375) pilD 3598286..3599149 (+) 864 WP_022995250.1 A24 family peptidase Machinery gene
  P40_RS16585 (P40_16380) coaE 3599364..3599969 (+) 606 WP_022995249.1 dephospho-CoA kinase -
  P40_RS16590 (P40_16385) - 3599959..3600153 (+) 195 WP_022995248.1 DNA gyrase inhibitor YacG -
  P40_RS16595 (P40_16390) - 3600154..3601104 (-) 951 WP_080531405.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62322.21 Da        Isoelectric Point: 5.7788

>NTDB_id=153546 P40_RS16570 WP_080531404.1 3595252..3596964(+) (pilB) [Alloalcanivorax xenomutans strain P40]
MSSPQSSLSGLARRLVNEGHLSTEQVMEAAGAARREQMPLVRRLVGSGLLTAATVARASAEEFGDPVIDLDAFSPDVLVK
DLVDTKLIARHTVLPLYKRGSRLFVAVSDPTNLPALEEIRFNTGLNVETVIVEDDKLTRWIDRLESEAEGKAFADLDEGL
DNLDVANGEPAADAKDEANGADDAPIVRFVNKLLLDAIKGGASDLHFEPYEKTYRVRFRQDGVLQTMSKPPINSASKIAA
RLKVMARLDISERRVPQDGRIKLKISKNRAIDFRVNTCPTLFGEKIVLRILDPDSAKMGIDALGYEPEQKDMYMKALNQP
QGMILVTGPTGSGKTVSLYTGVNILNTPERNISTAEDPVEINLEGINQVNVNPKQGLDFSSALRAFLRQDPDVILVGEIR
DLETAEIAVKAAQTGHLVLSTLHTNSAPETLTRLRNMGVPSFNIATSVSLIIAQRLARRLCSHCKEAVDVPKPSLLAMGF
TEHDIESGFTVFGPKGCQKCNNGYKGRVGIYEVVRVTDGIARIIMQDGNSIEIADQCRKEGFNDLRRSGLLKVMQGVTSL
EEMDRVTSGH

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=153546 P40_RS16570 WP_080531404.1 3595252..3596964(+) (pilB) [Alloalcanivorax xenomutans strain P40]
ATGAGCAGTCCTCAATCATCCCTGAGCGGCCTCGCCCGCCGCCTGGTCAACGAAGGCCATCTGTCCACCGAGCAGGTGAT
GGAAGCGGCCGGCGCCGCCCGCCGGGAGCAGATGCCGCTGGTCCGGCGTCTGGTGGGCTCGGGTCTGCTGACCGCGGCCA
CCGTGGCACGGGCTTCCGCCGAGGAGTTCGGGGATCCGGTGATAGATCTGGACGCGTTTTCCCCGGATGTCCTGGTCAAG
GACCTGGTGGATACCAAGCTGATCGCCCGCCACACCGTCCTGCCCCTGTACAAAAGGGGCAGCCGTTTGTTCGTCGCCGT
CTCCGACCCCACCAATCTGCCGGCGCTGGAGGAAATCCGCTTCAATACCGGCCTCAACGTGGAAACAGTGATTGTCGAGG
ACGACAAGCTGACCCGTTGGATCGACCGGCTGGAGTCGGAGGCGGAGGGCAAGGCGTTCGCCGATCTCGACGAGGGACTG
GACAATCTGGACGTGGCCAACGGTGAACCGGCCGCGGACGCCAAGGACGAGGCCAACGGCGCCGACGATGCCCCCATCGT
TCGCTTCGTCAACAAGCTGCTGCTGGACGCCATCAAGGGCGGCGCCTCGGACCTGCACTTCGAACCCTACGAAAAGACCT
ACCGGGTACGGTTCCGTCAGGACGGTGTGTTGCAGACCATGTCCAAGCCGCCGATCAACAGCGCCAGCAAGATCGCCGCC
CGGCTCAAGGTGATGGCGCGCCTGGATATTTCCGAACGACGGGTTCCCCAGGACGGTCGCATCAAGCTGAAGATTTCCAA
GAATCGCGCCATCGATTTCCGGGTCAATACCTGCCCCACTCTGTTTGGCGAAAAGATCGTGCTGCGGATTCTGGATCCGG
ACAGCGCCAAGATGGGTATTGATGCGCTGGGCTATGAACCCGAGCAGAAAGACATGTACATGAAGGCCCTGAACCAGCCC
CAGGGCATGATCCTGGTCACCGGCCCCACCGGTTCCGGTAAGACCGTGTCCCTTTATACCGGCGTGAACATTCTCAACAC
GCCGGAGCGCAACATCTCCACCGCCGAGGATCCGGTGGAAATCAACCTGGAAGGCATCAATCAGGTCAACGTGAATCCCA
AGCAGGGGCTGGATTTCTCGTCCGCGCTGCGGGCGTTTCTGCGTCAGGATCCCGACGTCATTCTGGTGGGGGAGATCCGC
GATCTGGAAACCGCGGAAATCGCCGTGAAAGCGGCCCAGACCGGCCACCTGGTGTTATCGACCCTGCACACCAACAGCGC
TCCGGAAACCCTGACCCGTCTGCGTAACATGGGGGTGCCGTCCTTCAATATCGCCACTTCGGTGAGTCTGATCATCGCCC
AGCGCCTGGCCCGCCGCCTTTGCAGCCACTGCAAGGAAGCGGTGGATGTGCCCAAACCCTCGCTGCTTGCGATGGGTTTC
ACGGAACATGACATCGAGAGTGGCTTTACTGTGTTCGGCCCGAAAGGCTGCCAAAAATGCAATAACGGCTACAAGGGCCG
GGTGGGGATCTATGAGGTGGTCCGGGTCACGGACGGTATTGCCCGCATCATCATGCAGGATGGCAATTCCATCGAGATCG
CCGACCAGTGCCGCAAGGAAGGCTTCAACGATTTGCGCCGCTCCGGGCTGCTCAAGGTGATGCAGGGGGTCACCAGTCTG
GAGGAAATGGACCGGGTCACCAGCGGCCACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.742

99.825

0.626
  pilB Acinetobacter baylyi ADP1

61.456

98.772

0.607
  pilB Legionella pneumophila strain ERS1305867

52.705

100

0.53
  pilF Neisseria gonorrhoeae MS11

49.291

98.947

0.488
  pilB Vibrio cholerae strain A1552

54.403

89.649

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

50.659

93.158

0.472
  pilB Vibrio campbellii strain DS40M4

50.76

92.281

0.468
  pilB/pilB1 Synechocystis sp. PCC 6803

41.667

90.526

0.377
  pilF Thermus thermophilus HB27

38.559

97.368

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.228

92.456

0.372

Multiple sequence alignment