Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   lpt_RS05060 Genome accession   NZ_CP012019
Coordinates   1122313..1124409 (+) Length   698 a.a.
NCBI ID   WP_061483600.1    Uniprot ID   -
Organism   Legionella pneumophila subsp. pneumophila strain Toronto-20052     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1117313..1129409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  lpt_RS05035 (lpt_05020) ravI 1118075..1119082 (-) 1008 WP_027266406.1 Dot/Icm T4SS effector RavI -
  lpt_RS05040 (lpt_05025) pilM 1119499..1120563 (+) 1065 WP_027266405.1 type IV pilus assembly protein PilM Machinery gene
  lpt_RS05045 (lpt_05030) pilN 1120567..1121115 (+) 549 WP_010946663.1 PilN domain-containing protein Machinery gene
  lpt_RS05050 (lpt_05035) pilO 1121129..1121728 (+) 600 WP_011215096.1 type 4a pilus biogenesis protein PilO Machinery gene
  lpt_RS05055 (lpt_05040) pilP 1121725..1122309 (+) 585 WP_027266404.1 pilus assembly protein PilP Machinery gene
  lpt_RS05060 (lpt_05045) pilQ 1122313..1124409 (+) 2097 WP_061483600.1 type IV pilus secretin PilQ Machinery gene
  lpt_RS05065 (lpt_05050) aroK 1124856..1125383 (+) 528 WP_010946667.1 shikimate kinase AroK -
  lpt_RS05070 (lpt_05055) aroB 1125370..1126479 (+) 1110 WP_013101269.1 3-dehydroquinate synthase -
  lpt_RS05075 (lpt_05060) - 1126476..1127927 (+) 1452 WP_027219627.1 SPOR domain-containing protein -
  lpt_RS05080 (lpt_05065) - 1128036..1128467 (+) 432 WP_010946670.1 universal stress protein -

Sequence

Protein


Download         Length: 698 a.a.        Molecular weight: 77501.78 Da        Isoelectric Point: 9.5395

>NTDB_id=150382 lpt_RS05060 WP_061483600.1 1122313..1124409(+) (pilQ) [Legionella pneumophila subsp. pneumophila strain Toronto-20052]
MRKIIAILILISASLSMAYSKDNSLISVKVLPLPENRVRIDFQFSKPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKT
KTIQLGSLHSYNIVAVGDRVRAILELDRSVSYSGSTAGSVYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTRIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQAL
DIILTTQGLDKRRKGNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSLLSDRGKIS
VDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
NVVPLERRLNLDLAAAPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQE
ATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKN
KVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK

Nucleotide


Download         Length: 2097 bp        

>NTDB_id=150382 lpt_RS05060 WP_061483600.1 1122313..1124409(+) (pilQ) [Legionella pneumophila subsp. pneumophila strain Toronto-20052]
GTGAGAAAAATTATCGCGATTTTGATATTAATTAGTGCCAGTTTGAGTATGGCATATTCTAAAGATAATTCGCTCATTTC
AGTAAAAGTATTACCATTGCCTGAAAACAGAGTGCGCATTGATTTTCAATTTTCCAAACCCTTAAAGCAGTTGCCCGCAA
GCTTTATTACTCAAAAGCCCCCACGTTTGGTGTTAGATTTTATCAGCACTGATATGCAAATTCCTTCAGATCAGAAAACT
AAAACCATTCAATTAGGCTCATTACATAGCTATAACATTGTTGCTGTTGGGGATAGAGTAAGAGCCATATTGGAGCTGGA
TCGTTCAGTGTCCTATTCTGGCAGTACGGCGGGTTCCGTTTATAGCCTTGTCCTGAATGGTAAAAGCAATGATTTATTTG
AAAATAGCAAGGAAGTATTCATAACGAACCAGGTTGTGAACGCCAAGCATGAAATTAAAAGAATTGATTTTCGTGGTATA
GAAAAGCAAGGCGGTAGAGTTTTAATTGATGTTTCTGATACTGGTATTCCAATTGATGTAACTCAAGTTGGGAAAGAAAT
AGTCGTTAATTTCCTGAATACCAGGATCCCTTTGAATTTAATGAAACGGTATGATGTTTCTGATTTTCACAGCCCTGTGC
AGATTATTACTATGCAACAGGAAGGAAAAAAGGTTCGTATGACCATTCTCAGCAAAGGGGATTATGGGCATTTTGTTTAT
CAGGTCAATAAACAATTTATGGTCGATGTCTTTCCTTTAACTGCGGAAGAAATTCGTCAGGCGAAATTGAAGAAACAGGT
ATTTACCGGTAAACGGATTTCTTTGAATTTCCAGAATATTAGTATTCGAGCTGTGCTTCAACTGCTGGCAGATTTTACCG
GAATCAATATGGTTGTCAGCGACAAAGTGCAAGGAGATATTACGCTAAGATTAAATGATATTCCCTGGGATCAGGCGCTG
GATATCATTTTAACCACACAAGGGTTAGATAAGCGCAGAAAGGGCAACGTCATGTTGATAGATACCAAGGCCTCCATGGA
TAAAATGGAGGAAGAACAACTCAAAAGTCAACAGACAATTGAAAAACTTGAGCCTATTCGTTCTGATTTAATACAGATTA
ATTATGCAAAGGCAGCAGATATTGCTGTTTTGATCAAGGATAAGCAAAATTCCCTATTATCAGACAGAGGAAAAATAAGT
GTTGATGCCAGAACGAATACAATCTGGATTCAGGATACTGGCACCAAAATTGAGGAAGTAAGGGAACTGATAAAGCAATT
GGATATCCCTGTTAAGCAAGTGCTTATTGAGGCAAGAATTGTTGAAGTAACTAAAGATTTTAGTCAAGATATTGGGATAC
GCTGGGGGGTATCAAGACCAACTCACTTAAGCGGTACATTGGAAGGTGCTAACGAGTTAGCGAGAGGAACAGCACCAGCT
AATGTTGTCCCCTTGGAAAGACGATTAAATCTTGATTTGGCAGCTGCTCCTTTAACTGGAGCTACACCGGCCTCGGTAGG
TATTGCTCTAGCCAAGCTTAATGATAACATTCTGCTTGATCTGGAATTGTCTGCTTTGGAAAGTGAAGGGCGTGCTGAAT
TAATATCCAGTCCCCGCCTGATTACTACGAATCAACAGGCAGCAGTCATCGAGTCTGGTGAGGAGATTCCCTATCAGGAG
GCAACATCGAGTGGAGCGACGGCTGTTGCATTTAAAAAAGCAGTGTTGAGTTTGAAAGTTACCCCGCAAATTACGCCTGA
CGGTAAAATTTTGATGGATTTACAGATAAATCAGGATACTCCTTCTCCACAAACATTCCTTGGTGTTCCAGCAATAATAA
CAAAGGAGATTCAGACGAATGTTCTGGTTAATAATGGACAAACCATAGTACTTGGGGGTATTTATAAGCAAGATAAGAAC
AAGGTAATCAATAGAATTCCTTTCTTTGGTGAGTTGCCTGTTGTCGGTATTTTATTTAGTAATAGACAAATCACATTGAA
AAATGAAGAATTACTTATTTTTATTACACCAAGAATAATAACGAATGCCCTATCGATAACAACGATAGAAGGACGTGAAA
AAGATGTTTATAAATAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(39-113)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-433)

Bacterial type II and III secretion system protein (Secretin)

(525-680)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain Lp02

99.713

100

0.997
  pilQ Legionella pneumophila strain ERS1305867

99.57

100

0.996
  pilQ Pseudomonas aeruginosa PAK

37.73

100

0.381
  comQ Acinetobacter baylyi ADP1

38.428

98.424

0.378
  pilQ Acinetobacter baumannii strain A118

34.959

100

0.37
  pilQ Acinetobacter baumannii D1279779

34.959

100

0.37

Multiple sequence alignment