Detailed information NTG0150378

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: lpt_RS05040
• Genome accession: NZ_CP012019
• Coordinates: 1119499..1120563 (+)
• Length: 354 a.a.
• NCBI ID: WP_027266405.1
• Uniprot ID: -
• Organism: Legionella pneumophila subsp. pneumophila strain Toronto-20052
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1114499..1125563

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
lpt_RS05030 (lpt_05015)	-	1115539..1117923 (-)	2385	WP_027266407.1	penicillin-binding protein 1A	-
lpt_RS05035 (lpt_05020)	ravI	1118075..1119082 (-)	1008	WP_027266406.1	Dot/Icm T4SS effector RavI	-
lpt_RS05040 (lpt_05025)	pilM	1119499..1120563 (+)	1065	WP_027266405.1	type IV pilus assembly protein PilM	Machinery gene
lpt_RS05045 (lpt_05030)	pilN	1120567..1121115 (+)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
lpt_RS05050 (lpt_05035)	pilO	1121129..1121728 (+)	600	WP_011215096.1	type 4a pilus biogenesis protein PilO	Machinery gene
lpt_RS05055 (lpt_05040)	pilP	1121725..1122309 (+)	585	WP_027266404.1	pilus assembly protein PilP	Machinery gene
lpt_RS05060 (lpt_05045)	pilQ	1122313..1124409 (+)	2097	WP_061483600.1	type IV pilus secretin PilQ	Machinery gene
lpt_RS05065 (lpt_05050)	aroK	1124856..1125383 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38790.81 Da
• Isoelectric Point: 5.0367

>NTDB_id=150378 lpt_RS05040 WP_027266405.1 1119499..1120563(+) (pilM) [Legionella pneumophila subsp. pneumophila strain Toronto-20052]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAITLKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=150378 lpt_RS05040 WP_027266405.1 1119499..1120563(+) (pilM) [Legionella pneumophila subsp. pneumophila strain Toronto-20052]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
GGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACCCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCGATTACCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGATAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCATGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.718	100	0.997
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407