Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ECTOBSL9_RS01950 Genome accession   NZ_CP011994
Coordinates   376075..377790 (+) Length   571 a.a.
NCBI ID   WP_063463642.1    Uniprot ID   A0A167A085
Organism   Ectothiorhodospira sp. BSL-9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 371075..382790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECTOBSL9_RS01930 (ECTOBSL9_0386) sucC 371288..372454 (-) 1167 WP_063463639.1 ADP-forming succinate--CoA ligase subunit beta -
  ECTOBSL9_RS16325 - 372602..372844 (+) 243 WP_025280629.1 PP0621 family protein -
  ECTOBSL9_RS01940 (ECTOBSL9_0388) - 372897..374537 (+) 1641 WP_205631987.1 nitrogen regulation protein NR(II) -
  ECTOBSL9_RS01945 (ECTOBSL9_0389) pilR 374559..375920 (+) 1362 WP_063463641.1 sigma-54 dependent transcriptional regulator Regulator
  ECTOBSL9_RS01950 (ECTOBSL9_0390) pilB 376075..377790 (+) 1716 WP_063463642.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ECTOBSL9_RS01955 (ECTOBSL9_0391) pilC 377794..379002 (+) 1209 WP_063463643.1 type II secretion system F family protein Machinery gene
  ECTOBSL9_RS01960 (ECTOBSL9_0392) pilD 379011..379874 (+) 864 WP_063465949.1 A24 family peptidase Machinery gene
  ECTOBSL9_RS01965 (ECTOBSL9_0393) coaE 379874..380473 (+) 600 WP_063463644.1 dephospho-CoA kinase -
  ECTOBSL9_RS01970 (ECTOBSL9_0394) zapD 380531..381373 (+) 843 WP_082829683.1 cell division protein ZapD -
  ECTOBSL9_RS01975 (ECTOBSL9_0395) - 381398..381907 (-) 510 WP_156500015.1 hypothetical protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63133.39 Da        Isoelectric Point: 5.3372

>NTDB_id=149960 ECTOBSL9_RS01950 WP_063463642.1 376075..377790(+) (pilB) [Ectothiorhodospira sp. BSL-9]
MSTPASSIPLSGLARRLVQDGQLNEADANRALEQSRKKRVPLVAYLVDEKLVEARSLAQSACEEFGTPVFDLDTLDRDHL
PKNLMDEKLVRQHQALPLYKRGNRLFVGLADPMNLTALDEIKFNTGIATEPVLVEQDKLVKLIEDALNDSGAMMDGLMDE
DLDDLQIKGDDDDRGQDVSESDIDDTPVVRFVNKVLLDAINKKASDIHFEPYEKNFRVRFRLDGVLSEVAHPPSNLAPRI
TARIKVMSRMDISERRIPQDGRIKMQLSKNRAIDFRVNTCPTLYGEKVVLRILDPTSAFLGIDQLGYEPHQKDLYMNALS
KPYGMILVTGPTGSGKTVSLYTGLGILNTADRNISTAEDPAEINMPGVNQVNVNPKVGLTFAAALRAFLRQDPDIIMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNDAPQTLTRLANMGVPSYNIASSILLIIAQRLGRRLCNHCKTPHDVPDDVLLEE
GFTEQEVKEGVKLFKPVGCDRCTLGYKGRVGIYQVLPISEGMQRLILEGGNAMQLAEQANKEGIADLRRSGLLKVKAGIT
SLEEINRITKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=149960 ECTOBSL9_RS01950 WP_063463642.1 376075..377790(+) (pilB) [Ectothiorhodospira sp. BSL-9]
ATGTCCACACCAGCCAGTTCCATCCCCCTGAGCGGCCTCGCCCGCAGACTTGTCCAGGATGGCCAGCTCAATGAGGCGGA
CGCTAACCGGGCTCTGGAGCAGTCGCGCAAGAAACGCGTGCCACTGGTGGCCTACCTGGTGGACGAGAAGCTGGTGGAAG
CCCGCAGCCTGGCACAATCCGCCTGCGAGGAATTTGGCACCCCGGTGTTCGACCTGGACACCCTGGACCGGGATCACCTG
CCCAAGAATCTCATGGACGAAAAGCTGGTCCGCCAGCACCAGGCCCTGCCCCTGTACAAGCGCGGCAATCGCCTGTTCGT
GGGCCTGGCCGACCCCATGAACCTCACCGCCCTGGACGAGATCAAGTTCAACACCGGCATCGCCACCGAGCCGGTGCTGG
TGGAACAGGACAAGCTGGTCAAGCTGATCGAGGATGCCCTCAACGATTCGGGGGCCATGATGGACGGCCTCATGGACGAG
GATCTGGACGATCTGCAGATCAAGGGGGATGACGACGACCGGGGCCAGGATGTTTCGGAAAGCGACATCGACGACACCCC
GGTGGTGCGCTTCGTCAACAAGGTGCTGCTGGATGCCATCAACAAGAAGGCCTCGGACATCCACTTCGAGCCCTACGAGA
AGAACTTCCGGGTGCGCTTTCGCCTGGACGGGGTGCTCTCGGAGGTGGCTCACCCGCCCTCCAACCTGGCCCCCCGCATC
ACCGCCCGCATCAAGGTCATGTCCCGCATGGACATTTCCGAGCGCCGCATCCCCCAGGATGGCCGCATCAAGATGCAGCT
TTCCAAGAACCGGGCCATCGACTTTCGGGTGAACACCTGCCCCACGCTGTACGGGGAGAAGGTGGTCCTGCGTATCCTGG
ACCCCACCAGTGCCTTTCTGGGGATCGACCAACTGGGCTATGAGCCGCACCAGAAAGACCTCTACATGAATGCCCTGAGC
AAGCCCTATGGCATGATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCGTGTCACTGTATACGGGCCTGGGCATCCT
CAACACGGCGGACCGCAACATCTCCACCGCCGAGGATCCGGCCGAGATCAACATGCCCGGGGTCAACCAGGTGAACGTGA
ACCCCAAGGTGGGCCTCACCTTCGCTGCTGCCCTGCGGGCTTTCTTGCGCCAGGATCCGGACATCATCATGGTGGGGGAG
ATCCGCGATCTGGAAACCGCCGAGATCGCCATCAAGGCGGCGCAGACGGGCCACCTGGTGCTGTCCACCCTGCACACCAA
CGACGCCCCACAGACCCTCACCCGGCTGGCCAACATGGGGGTGCCCTCCTACAACATCGCCTCCTCCATCCTCCTGATCA
TCGCCCAGCGACTGGGGCGCCGCCTGTGCAATCATTGCAAGACCCCCCACGATGTGCCCGACGATGTGCTGCTGGAGGAG
GGTTTCACCGAACAGGAGGTCAAGGAGGGTGTCAAGCTGTTCAAGCCGGTGGGATGCGACCGCTGCACCCTGGGTTACAA
GGGGCGTGTCGGCATCTATCAGGTGCTGCCCATCTCCGAAGGCATGCAGCGACTGATCCTGGAGGGGGGCAACGCCATGC
AGCTGGCCGAACAGGCCAACAAGGAAGGCATTGCGGACCTGCGGCGCTCGGGACTGCTCAAGGTCAAGGCCGGCATCACC
AGCCTTGAAGAAATCAACCGTATCACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-146)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A167A085

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.545

100

0.567
  pilB Acinetobacter baylyi ADP1

55.672

100

0.559
  pilB Legionella pneumophila strain ERS1305867

53.739

100

0.541
  pilF Neisseria gonorrhoeae MS11

49.735

98.949

0.492
  pilB Vibrio cholerae strain A1552

51.803

92.294

0.478
  pilB Vibrio campbellii strain DS40M4

48.057

99.124

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

47.452

99.65

0.473
  pilF Thermus thermophilus HB27

38.669

97.373

0.377

Multiple sequence alignment