Detailed information NTG0149962

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ECTOBSL9_RS01960
• Genome accession: NZ_CP011994
• Coordinates: 379011..379874 (+)
• Length: 287 a.a.
• NCBI ID: WP_063465949.1
• Uniprot ID: A0A167BKF9
• Organism: Ectothiorhodospira sp. BSL-9
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 374011..384874

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ECTOBSL9_RS01945 (ECTOBSL9_0389)	pilR	374559..375920 (+)	1362	WP_063463641.1	sigma-54 dependent transcriptional regulator	Regulator
ECTOBSL9_RS01950 (ECTOBSL9_0390)	pilB	376075..377790 (+)	1716	WP_063463642.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ECTOBSL9_RS01955 (ECTOBSL9_0391)	pilC	377794..379002 (+)	1209	WP_063463643.1	type II secretion system F family protein	Machinery gene
ECTOBSL9_RS01960 (ECTOBSL9_0392)	pilD	379011..379874 (+)	864	WP_063465949.1	A24 family peptidase	Machinery gene
ECTOBSL9_RS01965 (ECTOBSL9_0393)	coaE	379874..380473 (+)	600	WP_063463644.1	dephospho-CoA kinase	-
ECTOBSL9_RS01970 (ECTOBSL9_0394)	zapD	380531..381373 (+)	843	WP_082829683.1	cell division protein ZapD	-
ECTOBSL9_RS01975 (ECTOBSL9_0395)	-	381398..381907 (-)	510	WP_156500015.1	hypothetical protein	-
ECTOBSL9_RS01980 (ECTOBSL9_0396)	-	381920..382897 (-)	978	WP_063463646.1	Nudix family hydrolase	-
ECTOBSL9_RS01985 (ECTOBSL9_0397)	argJ	382915..384129 (-)	1215	WP_063463647.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 30983.78 Da
• Isoelectric Point: 7.7657

>NTDB_id=149962 ECTOBSL9_RS01960 WP_063465949.1 379011..379874(+) (pilD) [Ectothiorhodospira sp. BSL-9]
MASTPWLLLTVTGLFSLLIGSFLNVVIHRLPIMMERQWQSEARAWLQTQGQSGDGGEVAGPYNLVVPRSGCPHCGHHIKA
VENIPVLSYLFLRGRCSSCGARISSQYPLVEAATALLSMLVVWHFGFTWAGGAALLFTWALIALAVIDLRTTLLPDMITL
PMLWLGLIVNLPGLFTDATSSLIGAIAGYLSLWLIYHAFRLLTGKEGMGYGDFKLFALIGAWLGWQVLPLVILLSSLVGA
VVGIALIVARGRDRDIPIPFGPYLAAAGFIALLWGDAIIGVYLQALA

Nucleotide

• Length: 864 bp

>NTDB_id=149962 ECTOBSL9_RS01960 WP_063465949.1 379011..379874(+) (pilD) [Ectothiorhodospira sp. BSL-9]
ATGGCAAGTACCCCCTGGCTGCTGCTCACCGTGACCGGGCTGTTCAGCCTGCTCATCGGCAGTTTCCTTAATGTGGTGAT
CCACCGGCTGCCCATCATGATGGAGCGCCAGTGGCAGAGTGAGGCCCGGGCCTGGTTGCAGACCCAGGGGCAATCCGGTG
ACGGTGGCGAGGTGGCCGGTCCTTACAATCTGGTGGTGCCCCGCTCCGGCTGCCCCCATTGCGGGCACCACATCAAGGCG
GTGGAAAACATTCCGGTGCTGAGCTACCTGTTCCTGCGGGGTCGGTGCTCGTCCTGCGGTGCCCGCATCTCCAGCCAGTA
CCCGCTGGTGGAGGCCGCCACGGCGCTGCTGTCCATGCTGGTGGTGTGGCATTTCGGGTTCACCTGGGCCGGCGGCGCGG
CGCTGCTGTTCACCTGGGCCCTCATCGCCCTGGCGGTCATCGACCTGCGCACCACCCTGCTGCCGGACATGATCACCCTG
CCCATGCTCTGGCTGGGCCTGATCGTCAACCTGCCCGGCCTGTTCACCGATGCCACCAGCAGCCTCATCGGTGCCATCGC
CGGCTATCTCAGCCTGTGGCTGATCTATCATGCCTTCCGGTTGCTCACCGGCAAGGAGGGTATGGGCTATGGGGACTTCA
AGCTCTTTGCCCTGATCGGGGCCTGGCTGGGCTGGCAGGTCCTGCCACTGGTCATCCTGCTCTCCTCCCTGGTGGGTGCC
GTGGTGGGCATTGCCCTGATCGTGGCACGGGGGCGCGACCGCGACATCCCCATCCCGTTCGGCCCCTACCTGGCCGCCGC
CGGCTTCATCGCCCTGTTATGGGGAGATGCCATTATCGGGGTCTATCTGCAGGCCCTTGCCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (14-125)

3D structure

• AlphaFold DB: A0A167BKF9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.429	97.561	0.502
pilD	Vibrio campbellii strain DS40M4	47.203	99.652	0.47
pilD	Acinetobacter baumannii D1279779	47.826	96.167	0.46
pilD	Acinetobacter nosocomialis M2	47.464	96.167	0.456
pilD	Neisseria gonorrhoeae MS11	47.71	91.289	0.436