Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACG10_RS03440 Genome accession   NZ_CP011835
Coordinates   729736..731853 (+) Length   705 a.a.
NCBI ID   WP_198319010.1    Uniprot ID   -
Organism   Azotobacter chroococcum strain B3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 724736..736853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG10_RS03420 (ACG10_03350) comM 726820..727884 (+) 1065 WP_089167129.1 pilus assembly protein PilM Machinery gene
  ACG10_RS03425 (ACG10_03355) - 727885..728541 (+) 657 WP_089167130.1 PilN domain-containing protein -
  ACG10_RS03430 (ACG10_03360) pilO 728538..729161 (+) 624 WP_089167131.1 type 4a pilus biogenesis protein PilO -
  ACG10_RS03435 (ACG10_03365) pilP 729158..729682 (+) 525 WP_089167132.1 type 4a pilus biogenesis lipoprotein PilP -
  ACG10_RS03440 (ACG10_03370) pilQ 729736..731853 (+) 2118 WP_198319010.1 type IV pilus secretin PilQ family protein Machinery gene
  ACG10_RS03445 (ACG10_03375) aroK 731858..732376 (+) 519 WP_089167134.1 shikimate kinase AroK -
  ACG10_RS03450 (ACG10_03380) aroB 732428..733531 (+) 1104 WP_089167135.1 3-dehydroquinate synthase -
  ACG10_RS03455 (ACG10_03385) - 733539..735203 (+) 1665 WP_089167136.1 AAA family ATPase -

Sequence

Protein


Download         Length: 705 a.a.        Molecular weight: 75953.16 Da        Isoelectric Point: 4.6089

>NTDB_id=148908 ACG10_RS03440 WP_198319010.1 729736..731853(+) (pilQ) [Azotobacter chroococcum strain B3]
MNSTLSRLGISVLAALLSPELLAADLKALDVAALPGDRVELKLSFDEPVPAPRGYTIEQPARIALDLPGVANKLGTRYRE
LGVGNARSVTLVEAGGRTRMIVNLASLSPYSTRAEGNNLYVVIGESAAAPRQAGTAPSPVVTAAPVPSVKPYAPAGKTVQ
GIDFQRGEQGEGNVIIDLSSPGMNPDVQEQGGKIRLDFPGAQLPESLRARLDVKDFATPVQFVSASSEGGKASILIEPTG
MYDYLVYQTDNRLTVSVKPLTQENVAKRNADRFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGNITLRLQN
VPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELEAQQQIAELAPLRRELVQVNYAKAADIAKLFQSVTSGEE
GEEQRGSITVDERTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSFVNDQWSLYGKDG
AQSIDDGQVFLPGTDQVGNFTLEDGVAPVPFVDMGVANSTSGLGIGFVSDHLILDLQLSAMEKTGNGEVISQPKVLTSDK
ETAKILTGEEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDQADFSKAIGGVPPINKNEVNAKVLIG
DGETIVIGGVFSNTQAKSVDKVPFLGDLPFIGRLFRRDVVADSKSELLVFLTPRILNNQAIAVSR

Nucleotide


Download         Length: 2118 bp        

>NTDB_id=148908 ACG10_RS03440 WP_198319010.1 729736..731853(+) (pilQ) [Azotobacter chroococcum strain B3]
ATGAACAGCACCCTTTCGCGTCTTGGTATTTCAGTCCTGGCTGCCCTGCTGTCGCCGGAATTGTTGGCGGCCGACCTCAA
GGCTCTGGATGTGGCCGCCCTGCCTGGCGATCGGGTCGAGCTCAAGTTGTCCTTCGACGAGCCCGTGCCGGCGCCGCGCG
GCTATACCATCGAGCAGCCGGCCCGGATCGCCCTGGATCTGCCCGGTGTAGCCAACAAGCTGGGGACCCGGTATCGCGAG
CTGGGCGTCGGCAATGCCCGCAGCGTCACCCTGGTCGAGGCGGGCGGCCGCACCCGGATGATCGTCAACCTGGCGAGCCT
GTCGCCCTATAGCACGCGAGCCGAAGGCAACAACCTCTATGTGGTGATCGGCGAGTCGGCCGCCGCGCCCCGGCAGGCCG
GCACTGCGCCGTCACCGGTCGTCACGGCGGCTCCGGTTCCGTCGGTCAAGCCCTATGCGCCGGCCGGCAAGACCGTCCAG
GGCATCGACTTCCAGCGCGGCGAGCAGGGCGAAGGCAACGTTATCATCGATCTGTCGAGTCCGGGCATGAATCCCGATGT
CCAGGAGCAGGGTGGCAAGATCCGCCTGGACTTCCCGGGTGCGCAGTTGCCGGAATCCCTGCGGGCACGTCTGGATGTCA
AGGATTTCGCTACACCCGTGCAGTTCGTGAGTGCTTCGAGTGAGGGTGGCAAGGCCAGCATCCTGATTGAGCCGACCGGC
ATGTACGACTATCTGGTCTACCAGACCGACAACCGCCTGACCGTCAGCGTCAAGCCGCTGACCCAGGAAAATGTCGCCAA
GCGCAATGCCGACCGCTTCGCCTATACCGGCGAGAAGCTGTCGCTGAACTTCCAGGATATCGATGTCCGCTCGGTCCTGC
AGCTGATCGCCGACTTCACCAACCTCAACCTGGTGGCCAGCGACACCGTGCAGGGCAATATCACCCTGCGCCTGCAGAAT
GTGCCCTGGGACCAGGCGCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTCGGCAATGTCCTGCTGGT
GGCGCCGGCCGACGAAATCGCGGCGCGCGAGCGTCAGGAGCTGGAGGCGCAGCAGCAGATCGCCGAGCTGGCCCCCCTGC
GCCGCGAGCTGGTCCAGGTGAACTACGCCAAGGCAGCGGATATCGCCAAGCTGTTCCAATCGGTCACCAGCGGCGAAGAG
GGTGAAGAGCAGCGCGGTTCCATCACCGTCGACGAGCGGACCAACAACATCATCGCCTACCAGACCCAGGAGCGTCTGGA
CGAGCTGCGACGGATCGTCGCCCAGCTGGACATTCCGGTGCGCCAGGTGATGATCGAGGCACGCATCGTCGAGGCCAATG
TCGATTACGACAAGAGCCTGGGGGTGCGCTGGGGCGGCAGCTTCGTGAACGACCAGTGGAGCCTGTATGGCAAGGATGGT
GCGCAATCCATCGATGACGGGCAGGTGTTTCTGCCTGGGACCGATCAGGTCGGCAACTTCACCCTGGAGGATGGCGTGGC
GCCGGTCCCCTTCGTCGACATGGGCGTGGCGAACAGTACATCGGGCCTTGGCATCGGCTTCGTTTCCGACCACCTGATTC
TCGACCTTCAACTGTCGGCCATGGAAAAGACCGGCAATGGAGAGGTCATTTCCCAGCCCAAGGTCCTCACTTCGGACAAG
GAGACGGCGAAGATCCTGACCGGCGAGGAGATCCCCTACCAGGAGGCCAGCTCCAGCGGTGCGACCAGCACCTCCTTCAA
GGAGGCGGCGCTGTCGCTGGAGGTGACGCCGCAGATCACCCCCGACAACCGCATCATCATGGAGGTGAAGGTGACCAAGG
ATCAGGCGGACTTCTCCAAGGCGATAGGCGGAGTGCCGCCGATCAACAAGAACGAGGTCAACGCCAAGGTGCTGATCGGC
GACGGCGAAACCATCGTGATCGGCGGAGTCTTCTCCAATACCCAGGCCAAGTCCGTGGACAAGGTGCCCTTCCTCGGCGA
CCTGCCGTTCATCGGACGTCTGTTCCGCCGCGACGTGGTCGCCGACAGCAAATCCGAGCTGCTTGTTTTCCTGACTCCGC
GTATTCTTAACAACCAGGCTATTGCGGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II and III secretion system protein (Secretin)

(540-696)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

75.314

100

0.766
  comQ Acinetobacter baylyi ADP1

42.611

98.865

0.421
  pilQ Acinetobacter baumannii strain A118

40.647

100

0.41
  pilQ Acinetobacter baumannii D1279779

40.647

100

0.41
  pilQ Legionella pneumophila strain ERS1305867

38.109

96.028

0.366
  pilQ Legionella pneumophila strain Lp02

37.814

96.028

0.363

Multiple sequence alignment