Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   ACG10_RS03420 Genome accession   NZ_CP011835
Coordinates   726820..727884 (+) Length   354 a.a.
NCBI ID   WP_089167129.1    Uniprot ID   -
Organism   Azotobacter chroococcum strain B3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 721820..732884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG10_RS03410 (ACG10_03340) - 722846..724114 (+) 1269 WP_089167127.1 malic enzyme-like NAD(P)-binding protein -
  ACG10_RS03415 (ACG10_03345) - 724174..726639 (-) 2466 WP_198319009.1 penicillin-binding protein 1A -
  ACG10_RS03420 (ACG10_03350) comM 726820..727884 (+) 1065 WP_089167129.1 pilus assembly protein PilM Machinery gene
  ACG10_RS03425 (ACG10_03355) - 727885..728541 (+) 657 WP_089167130.1 PilN domain-containing protein -
  ACG10_RS03430 (ACG10_03360) pilO 728538..729161 (+) 624 WP_089167131.1 type 4a pilus biogenesis protein PilO -
  ACG10_RS03435 (ACG10_03365) pilP 729158..729682 (+) 525 WP_089167132.1 type 4a pilus biogenesis lipoprotein PilP -
  ACG10_RS03440 (ACG10_03370) pilQ 729736..731853 (+) 2118 WP_198319010.1 type IV pilus secretin PilQ family protein Machinery gene
  ACG10_RS03445 (ACG10_03375) aroK 731858..732376 (+) 519 WP_089167134.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37732.46 Da        Isoelectric Point: 4.7199

>NTDB_id=148907 ACG10_RS03420 WP_089167129.1 726820..727884(+) (comM) [Azotobacter chroococcum strain B3]
MLGLFNKKAGSLLGIDISSTTVKLIELSRSGNRYRVEAYAVEPLPANAVVEKNIVDLDGVGQTLSKVLAKAKTGTRNAAV
AVAGSVVITKTVEMDSGLSEDEMETQLKLEADQYIPYPLEEVAIDFEIQGPLPRSPGRAEVLLAACRKENVEAREAVLAL
AGLTAKVVDVEAYAMERACGLLVDQLGGDPAGLIVAVVDIGATMMTLSVMHQGRTLYTREQLFGGKQLTDEIQRRYGLSM
AEAGLAKKKGGLPADYDSEVLQPFKDAVVQQVSRSLQFFFAAGQYNQVDYILLAGGTSSLPGLDQLIQQKTGTRTLVANP
FAEMALSPKVNASALVSDAPAMMVACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=148907 ACG10_RS03420 WP_089167129.1 726820..727884(+) (comM) [Azotobacter chroococcum strain B3]
GTGCTAGGGCTCTTCAACAAGAAAGCGGGTTCATTACTGGGGATCGATATTAGTTCGACCACGGTCAAGCTTATCGAATT
GAGTCGTTCAGGTAACCGTTACCGGGTAGAGGCCTACGCTGTCGAGCCGCTTCCTGCCAATGCCGTCGTCGAGAAGAACA
TTGTCGATCTCGATGGTGTCGGCCAGACCTTGAGCAAGGTGCTGGCCAAGGCCAAGACCGGAACCAGGAACGCGGCCGTG
GCCGTGGCGGGTTCCGTGGTGATTACCAAGACCGTCGAGATGGATTCCGGGCTTTCCGAGGATGAAATGGAAACCCAGTT
GAAGCTCGAGGCCGACCAATACATTCCCTATCCCCTCGAGGAAGTGGCGATCGACTTCGAGATCCAGGGGCCGCTGCCAC
GCAGCCCCGGACGCGCCGAGGTGCTGCTCGCCGCCTGCCGCAAGGAGAACGTCGAGGCCCGCGAGGCGGTGCTGGCACTG
GCCGGCCTGACGGCCAAGGTCGTCGATGTCGAGGCCTATGCCATGGAGCGCGCCTGCGGCCTGCTGGTCGACCAACTGGG
CGGCGATCCGGCGGGGTTGATCGTAGCTGTCGTCGATATCGGCGCGACCATGATGACGCTCAGCGTAATGCATCAAGGTC
GCACCCTCTATACCCGCGAGCAGCTGTTCGGCGGCAAGCAGCTCACCGACGAAATCCAGCGCCGCTATGGCCTGTCCATG
GCCGAAGCCGGTCTGGCCAAGAAGAAAGGCGGACTTCCAGCAGACTACGACAGCGAGGTTTTGCAGCCGTTCAAGGACGC
TGTCGTACAGCAGGTTTCCCGTTCGCTGCAGTTCTTTTTCGCGGCGGGCCAGTACAACCAGGTCGACTACATCCTGCTGG
CGGGAGGGACCTCTTCCCTGCCGGGACTTGATCAGCTGATCCAGCAGAAGACCGGGACCCGGACGCTGGTGGCCAACCCG
TTTGCCGAGATGGCGTTGAGCCCCAAGGTGAATGCCTCGGCGCTGGTCAGCGATGCTCCAGCCATGATGGTTGCCTGCGG
TCTGGCGATGAGGAGTTTCGATTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

54.366

100

0.545
  pilM Acinetobacter baumannii D1279779

54.085

100

0.542
  comM Acinetobacter baylyi ADP1

52.113

100

0.523
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.449

Multiple sequence alignment