Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BCV59_RS11080 Genome accession   NZ_CP014583
Coordinates   1919594..1920979 (+) Length   461 a.a.
NCBI ID   WP_001025153.1    Uniprot ID   -
Organism   Escherichia coli strain CFSAN004176     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1914594..1925979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCV59_RS11050 (BCV59_09955) aroP 1915143..1916516 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  BCV59_RS11055 (BCV59_09960) ampE 1916559..1917412 (-) 854 Protein_1908 beta-lactamase regulator AmpE -
  BCV59_RS11060 (BCV59_09965) ampD 1917409..1917960 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BCV59_RS11065 (BCV59_09970) nadC 1918048..1918941 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BCV59_RS11075 (BCV59_09980) pilA 1919144..1919584 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  BCV59_RS11080 (BCV59_09985) pilB 1919594..1920979 (+) 1386 WP_001025153.1 type II secretion system protein GspE Machinery gene
  BCV59_RS11085 (BCV59_09990) hofC 1920969..1922171 (+) 1203 WP_024225967.1 protein transport protein HofC -
  BCV59_RS11090 (BCV59_09995) guaC 1922206..1923249 (-) 1044 WP_001217338.1 GMP reductase -
  BCV59_RS31060 - 1923405..1923449 (-) 45 WP_120795372.1 protein YacM -
  BCV59_RS11095 (BCV59_10000) coaE 1923474..1924094 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  BCV59_RS11100 (BCV59_10005) zapD 1924094..1924837 (+) 744 WP_001194731.1 cell division protein ZapD -
  BCV59_RS11105 (BCV59_10010) yacG 1924847..1925044 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  BCV59_RS11115 (BCV59_10020) mutT 1925151..1925540 (-) 390 WP_000736004.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50577.23 Da        Isoelectric Point: 6.4971

>NTDB_id=148696 BCV59_RS11080 WP_001025153.1 1919594..1920979(+) (pilB) [Escherichia coli strain CFSAN004176]
MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=148696 BCV59_RS11080 WP_001025153.1 1919594..1920979(+) (pilB) [Escherichia coli strain CFSAN004176]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGTGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTTAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGCGTTGCTCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCAGCAGCAAGGGGAGCCCATCCATATTCCAGACACTGTATGGC
CGTCGCCGCTGCCCCACTGGCAAGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

42.798

100

0.451
  pilB Legionella pneumophila strain ERS1305867

49.871

83.948

0.419
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

39.957

100

0.406
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

47.013

83.514

0.393
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilF Neisseria gonorrhoeae MS11

44.675

83.514

0.373
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Thermus thermophilus HB27

41.542

87.202

0.362