Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BCV59_RS11075 Genome accession   NZ_CP014583
Coordinates   1919144..1919584 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain CFSAN004176     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1914144..1924584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCV59_RS11050 (BCV59_09955) aroP 1915143..1916516 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  BCV59_RS11055 (BCV59_09960) ampE 1916559..1917412 (-) 854 Protein_1908 beta-lactamase regulator AmpE -
  BCV59_RS11060 (BCV59_09965) ampD 1917409..1917960 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BCV59_RS11065 (BCV59_09970) nadC 1918048..1918941 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BCV59_RS11075 (BCV59_09980) pilA 1919144..1919584 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  BCV59_RS11080 (BCV59_09985) pilB 1919594..1920979 (+) 1386 WP_001025153.1 type II secretion system protein GspE Machinery gene
  BCV59_RS11085 (BCV59_09990) hofC 1920969..1922171 (+) 1203 WP_024225967.1 protein transport protein HofC -
  BCV59_RS11090 (BCV59_09995) guaC 1922206..1923249 (-) 1044 WP_001217338.1 GMP reductase -
  BCV59_RS31060 - 1923405..1923449 (-) 45 WP_120795372.1 protein YacM -
  BCV59_RS11095 (BCV59_10000) coaE 1923474..1924094 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=148695 BCV59_RS11075 WP_000360904.1 1919144..1919584(+) (pilA) [Escherichia coli strain CFSAN004176]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=148695 BCV59_RS11075 WP_000360904.1 1919144..1919584(+) (pilA) [Escherichia coli strain CFSAN004176]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGTGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363