Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   TP50_RS11255 Genome accession   NZ_CP011163
Coordinates   2500420..2501550 (+) Length   376 a.a.
NCBI ID   WP_003489921.1    Uniprot ID   A0AAI7ZGP8
Organism   Xanthomonas citri pv. aurantifolii strain FDC 1609     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2495420..2506550
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TP50_RS11230 (TP50_10945) - 2495534..2496175 (-) 642 WP_007973476.1 M23 family metallopeptidase -
  TP50_RS11235 (TP50_10950) - 2496278..2496703 (-) 426 WP_042805936.1 HU family DNA-binding protein -
  TP50_RS11240 (TP50_10955) proC 2497268..2498122 (-) 855 WP_007966170.1 pyrroline-5-carboxylate reductase -
  TP50_RS11245 (TP50_10960) - 2498167..2498859 (-) 693 WP_042675756.1 YggS family pyridoxal phosphate-dependent enzyme -
  TP50_RS11250 (TP50_10965) pilT 2499270..2500307 (+) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  TP50_RS11255 (TP50_10970) pilU 2500420..2501550 (+) 1131 WP_003489921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TP50_RS11260 (TP50_10975) - 2502055..2502729 (+) 675 WP_042675758.1 YitT family protein -
  TP50_RS11265 (TP50_10980) - 2502904..2503830 (-) 927 WP_173362056.1 DUF72 domain-containing protein -
  TP50_RS11270 (TP50_10985) - 2503838..2504410 (-) 573 WP_007973470.1 DNA-3-methyladenine glycosylase I -
  TP50_RS11280 (TP50_10995) - 2504700..2505266 (+) 567 WP_003489930.1 YqgE/AlgH family protein -
  TP50_RS11285 (TP50_11000) ruvX 2505259..2505726 (+) 468 WP_003489932.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42044.26 Da        Isoelectric Point: 6.8286

>NTDB_id=143057 TP50_RS11255 WP_003489921.1 2500420..2501550(+) (pilU) [Xanthomonas citri pv. aurantifolii strain FDC 1609]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=143057 TP50_RS11255 WP_003489921.1 2500420..2501550(+) (pilU) [Xanthomonas citri pv. aurantifolii strain FDC 1609]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGCCCGATTACCCAGACCCCGCTGACCGCGCAGCAAAGTCGCGATCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGTTTTCGTGTGAGCTGCTTCTACCAGCGTAATCAGGTGGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCTTGCCGCCGGTGATCAAGACGCTGTCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGGACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCATGCGATCGCCTTCGCCGAAACCGGCCACCTGGTGTTGTGTACGCTGCACGCCAACAACGCCAACCAGGCGATG
GATCGCATCATCAACTTCTTCCCCGAAGATCGCCGCAACCAGCTATTGATGGATCTGTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGATGGCCGCAGCCGTCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCTTGTTCGAGCTCTACCAGGCCGGCGAAATCAGCTACGAAGATGCGTTGCGCTACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTCTCGCAGGGTGGCGATGCCAAGACATTGTCGCAGGGCCTGGACGGTGTAGAGATTTCCG
AAGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.457

94.947

0.394
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364
  pilT Acinetobacter nosocomialis M2

39.826

91.489

0.364
  pilT Acinetobacter baumannii D1279779

39.826

91.489

0.364
  pilT Acinetobacter baumannii strain A118

39.826

91.489

0.364

Multiple sequence alignment