Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LC55x_RS18950 Genome accession   NZ_CP011130
Coordinates   4723256..4724983 (+) Length   575 a.a.
NCBI ID   WP_057922447.1    Uniprot ID   -
Organism   Lysobacter capsici strain 55     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4718256..4729983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LC55x_RS18930 (LC55x_4254) - 4718389..4720014 (+) 1626 WP_057922444.1 ATP-binding protein -
  LC55x_RS18935 (LC55x_4255) pilR 4720100..4721509 (+) 1410 WP_036104561.1 sigma-54 dependent transcriptional regulator Regulator
  LC55x_RS18940 (LC55x_4256) pilA2 4722060..4722485 (+) 426 WP_057922445.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LC55x_RS28400 - 4722646..4723071 (+) 426 WP_057922446.1 pilin -
  LC55x_RS18950 (LC55x_4257) pilB 4723256..4724983 (+) 1728 WP_057922447.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LC55x_RS18955 (LC55x_4258) pilC 4724987..4726258 (+) 1272 WP_057922448.1 type II secretion system F family protein Machinery gene
  LC55x_RS18960 (LC55x_4259) - 4726280..4727167 (+) 888 WP_036104586.1 A24 family peptidase -
  LC55x_RS18965 (LC55x_4260) coaE 4727171..4727791 (+) 621 WP_057922449.1 dephospho-CoA kinase -
  LC55x_RS18970 (LC55x_4261) - 4727942..4728916 (-) 975 WP_057922450.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62853.22 Da        Isoelectric Point: 6.4476

>NTDB_id=142621 LC55x_RS18950 WP_057922447.1 4723256..4724983(+) (pilB) [Lysobacter capsici strain 55]
MSSVATANLVGITGIARRLVMDGALDEVKAREAMSAASAERKPLASYLAEHRLATPAQLAAANSIEFGVPLFDPSTMDPG
QAPIRAISEELIRKHNALPLFKRGNKLFIGLADPTNTKALDEIKFQTNAAVEAILVDEELIRRTLDRWLETSDALGDAVG
DGEGLDNLEIGKDDDLNTTSDTGIDAKGDDTPVVKFINKVLVDAIRRGASDIHFEPYETDYRVRLRIDGLLKQVAKVPNK
LHSRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRMSTLPTLFGEKIVLRILDGSAARLGIEKLGYEDHQRELF
VNAVVKPYGMVLVTGPTGSGKTVSLYTALNILNDEQRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPYNITSSVTLVIAQRLARRLHDCKREVHLPEHA
LLAEGFTHEEIASGFKLYEPAGCPDCTEGYKGRTGIYQVMPMTDEIQAIVLEGGNAMQIAAAAIKSGVRDLRRSALDKVM
SGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=142621 LC55x_RS18950 WP_057922447.1 4723256..4724983(+) (pilB) [Lysobacter capsici strain 55]
ATGTCCAGCGTCGCGACCGCCAACTTGGTGGGTATCACCGGCATTGCCCGGCGTTTAGTAATGGATGGCGCCCTGGACGA
GGTCAAGGCGCGCGAAGCCATGAGCGCGGCCAGCGCTGAACGCAAGCCGCTGGCCTCTTATCTGGCCGAACACCGCTTGG
CCACTCCGGCCCAGCTCGCCGCCGCCAACTCGATCGAGTTCGGCGTGCCGCTGTTCGACCCGAGCACCATGGACCCGGGC
CAGGCCCCGATCCGGGCGATCAGCGAGGAACTGATCCGCAAGCACAACGCCTTGCCGCTGTTCAAGCGCGGCAACAAGCT
GTTCATCGGCCTGGCCGATCCGACCAACACCAAGGCCCTGGACGAGATCAAGTTCCAGACCAATGCCGCGGTCGAGGCGA
TCCTGGTCGATGAGGAACTGATCCGTCGCACCCTGGACCGTTGGCTGGAGACCTCCGACGCCCTGGGCGACGCCGTCGGC
GACGGCGAGGGCCTGGACAACCTGGAGATCGGCAAGGACGACGACCTCAACACCACCAGCGACACCGGCATCGACGCCAA
GGGCGACGACACCCCGGTGGTCAAGTTCATCAACAAGGTGCTGGTCGACGCGATCCGCCGCGGCGCGTCCGACATCCATT
TCGAACCCTACGAAACCGACTATCGGGTACGCCTGCGCATCGATGGCCTGCTCAAGCAGGTAGCGAAGGTTCCGAACAAG
CTGCACTCGCGCATTGCGGCGCGCCTGAAGGTCATGGCGCAACTCGACATCGCCGAGAAGCGAGTGCCGCAGGACGGACG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCATGAGCACCTTGCCGACCCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTCGACGGCAGCGCGGCGCGCCTCGGCATCGAGAAGCTGGGTTACGAAGACCATCAAAGAGAGTTGTTC
GTCAACGCGGTGGTCAAGCCCTACGGCATGGTGCTGGTCACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTCAACATCCTCAACGACGAACAACGCAATATCTCAACGGTCGAGGACCCGGTCGAAATCCGCGTGCCGGGCATCA
ACCAGGTGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGCCAGGATCCGGACGTG
ATCATGGTCGGCGAAATCCGCGACCTCGAGACCGCCGAGATCGCGATCAAGGCCGCCCAGACCGGCCACATGGTGCTGTC
GACCCTGCACACCAACGATGCGCCGCAGACCATCGCCCGCCTGATGAACATGGGCGTGGCGCCGTACAACATCACCTCGT
CGGTCACCCTGGTGATCGCACAGCGTCTCGCCCGCCGTCTGCACGACTGCAAGCGCGAGGTGCATCTGCCCGAACACGCG
CTGCTCGCCGAGGGGTTCACCCACGAGGAGATCGCGTCGGGTTTCAAGCTGTACGAGCCGGCGGGATGCCCGGACTGCAC
CGAAGGGTACAAGGGACGTACCGGTATCTATCAGGTCATGCCCATGACCGACGAAATCCAGGCCATCGTGCTCGAGGGGG
GCAACGCGATGCAGATCGCCGCCGCTGCGATCAAGTCCGGCGTGCGCGATCTGCGCCGCTCGGCCTTGGACAAAGTGATG
AGCGGCGTCACCAGCCTGGCCGAAATCAACCGCGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.36

98.957

0.548
  pilB Acinetobacter baylyi ADP1

55.379

98.609

0.546
  pilB Legionella pneumophila strain ERS1305867

50.613

99.304

0.503
  pilB Vibrio cholerae strain A1552

52.621

89.565

0.471
  pilF Neisseria gonorrhoeae MS11

47.619

98.609

0.47
  pilB Vibrio campbellii strain DS40M4

51.738

85.043

0.44
  pilB Vibrio parahaemolyticus RIMD 2210633

51.745

84.696

0.438
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.242

100

0.376
  pilF Thermus thermophilus HB27

39.509

92

0.363

Multiple sequence alignment