Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   VO64_RS20490 Genome accession   NZ_CP011117
Coordinates   4360883..4362091 (-) Length   402 a.a.
NCBI ID   WP_046070862.1    Uniprot ID   A0AAU8TRN8
Organism   Pseudomonas synxantha strain LBUM223     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4355883..4367091
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VO64_RS20455 (VO64_3865) - 4357216..4357389 (-) 174 WP_046070858.1 DUF3094 family protein -
  VO64_RS20460 (VO64_3866) - 4357478..4358107 (-) 630 WP_043049309.1 DUF1780 domain-containing protein -
  VO64_RS20465 (VO64_3867) - 4358237..4358926 (+) 690 WP_046070859.1 energy-coupling factor ABC transporter permease -
  VO64_RS20470 (VO64_3868) - 4358991..4359206 (+) 216 WP_003171680.1 hypothetical protein -
  VO64_RS20475 (VO64_3869) yacG 4359203..4359403 (-) 201 WP_043049311.1 DNA gyrase inhibitor YacG -
  VO64_RS20480 (VO64_3870) coaE 4359400..4360023 (-) 624 WP_046070860.1 dephospho-CoA kinase -
  VO64_RS20485 (VO64_3871) pilD 4360020..4360886 (-) 867 WP_046070861.1 prepilin peptidase Machinery gene
  VO64_RS20490 (VO64_3872) pilC 4360883..4362091 (-) 1209 WP_046070862.1 type II secretion system F family protein Machinery gene
  VO64_RS20495 (VO64_3873) - 4362293..4362706 (+) 414 WP_046070863.1 pilin -
  VO64_RS20505 (VO64_3874) - 4362993..4363298 (+) 306 WP_046070864.1 DUF6388 family protein -
  VO64_RS20510 (VO64_3875) nadC 4363417..4364265 (-) 849 WP_046070865.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VO64_RS20515 (VO64_3876) - 4364511..4366664 (+) 2154 WP_046070866.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43570.99 Da        Isoelectric Point: 10.1121

>NTDB_id=142503 VO64_RS20490 WP_046070862.1 4360883..4362091(-) (pilC) [Pseudomonas synxantha strain LBUM223]
MNDTSTIYAWEGINRKGRRVSGQIAGNDLALIKAQLRQQGICPERVRKKPAPRPTFAPRILPADIALFTRQLATLLKAGI
PLLHAFDIIREGVENRQVRDLVEALKQQIASGNSLADALRLHPRYFDDLYCNLVSTGEQAGALELLLERIATHLEKSQRL
KARIKKAMTYPVAVLVVATLVSAVLLIHVVPQFQNLFAGVNGQLPGFTLAVIALSEFLQQAWWVLALGLGASLAGLRQAY
RAYPGFRHRLEAGLLKMPLAGKLLHNSAVARYARTLSTTFAAGVPLVQALGSVAGAVGNGPFKQAIERMRHDVSTGMQLN
QSMASSGLFPGMAIQMTAIGEESGTLDRMLEKVANHYEADVDTLVDNLTSLMEPLIMVVLGGIVGALVIALYLPVFQLGT
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=142503 VO64_RS20490 WP_046070862.1 4360883..4362091(-) (pilC) [Pseudomonas synxantha strain LBUM223]
ATGAATGACACATCAACGATCTACGCCTGGGAAGGCATCAACCGCAAGGGACGCAGGGTGTCCGGGCAAATTGCAGGCAA
TGACCTTGCGCTGATCAAGGCACAATTGCGCCAGCAAGGGATCTGTCCCGAGCGTGTGCGTAAAAAGCCTGCGCCTCGGC
CGACGTTCGCCCCACGTATATTGCCTGCGGATATCGCCCTGTTCACCCGCCAGTTGGCCACATTGCTCAAGGCCGGTATC
CCGCTGCTACATGCCTTCGACATCATCAGAGAGGGCGTCGAGAACCGGCAGGTGCGTGACCTGGTCGAAGCCTTGAAACA
GCAGATCGCCAGTGGCAACAGCTTGGCCGATGCGCTGCGCCTACATCCCCGTTATTTCGATGACCTGTACTGCAACTTGG
TGTCCACCGGGGAACAGGCCGGCGCCCTGGAATTGCTGCTGGAGCGAATAGCGACTCACCTGGAGAAAAGCCAACGGCTC
AAAGCCAGGATCAAAAAGGCCATGACCTACCCTGTTGCGGTACTGGTGGTTGCCACGCTGGTCAGCGCGGTGCTGTTGAT
ACACGTAGTGCCGCAATTCCAGAACCTGTTCGCCGGGGTCAACGGCCAATTGCCCGGGTTTACCTTGGCGGTCATTGCCC
TGTCCGAATTCCTGCAACAGGCGTGGTGGGTGCTGGCGCTGGGCTTGGGTGCGTCGCTGGCAGGGCTTCGCCAGGCTTAC
CGTGCTTACCCAGGCTTTCGCCATCGGCTGGAAGCGGGTTTGTTGAAAATGCCCCTGGCAGGCAAACTGCTGCACAACTC
TGCCGTCGCTCGTTACGCCCGCACGCTTTCAACCACGTTCGCAGCGGGCGTGCCCCTCGTGCAGGCATTGGGCTCGGTGG
CCGGCGCCGTCGGCAATGGACCATTCAAACAGGCAATTGAACGTATGCGCCACGATGTATCCACAGGCATGCAATTGAAC
CAGTCCATGGCCAGCAGTGGCCTGTTTCCAGGCATGGCGATCCAGATGACGGCCATCGGTGAAGAATCCGGCACGCTGGA
CCGAATGTTGGAAAAAGTCGCGAACCACTACGAGGCGGACGTGGACACCTTGGTCGATAACCTCACCAGCCTGATGGAAC
CGCTGATCATGGTGGTACTGGGGGGCATTGTCGGGGCGTTGGTGATCGCCCTGTACCTGCCGGTCTTTCAACTGGGTACG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.818

98.507

0.56
  pilC Acinetobacter baylyi ADP1

50.253

98.507

0.495
  pilC Legionella pneumophila strain ERS1305867

48.363

98.756

0.478
  pilC Acinetobacter baumannii D1279779

48.101

98.259

0.473
  pilC Thermus thermophilus HB27

37.406

99.751

0.373
  pilC Vibrio cholerae strain A1552

37.817

98.01

0.371
  pilG Neisseria gonorrhoeae MS11

38.845

94.776

0.368
  pilG Neisseria meningitidis 44/76-A

38.058

94.776

0.361

Multiple sequence alignment