Detailed information NTG0142502

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: VO64_RS20485
• Genome accession: NZ_CP011117
• Coordinates: 4360020..4360886 (-)
• Length: 288 a.a.
• NCBI ID: WP_046070861.1
• Uniprot ID: A0AAU8U0Z6
• Organism: Pseudomonas synxantha strain LBUM223
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4355020..4365886

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VO64_RS20450 (VO64_3864)	-	4355857..4357155 (-)	1299	WP_046070857.1	NAD(P)/FAD-dependent oxidoreductase	-
VO64_RS20455 (VO64_3865)	-	4357216..4357389 (-)	174	WP_046070858.1	DUF3094 family protein	-
VO64_RS20460 (VO64_3866)	-	4357478..4358107 (-)	630	WP_043049309.1	DUF1780 domain-containing protein	-
VO64_RS20465 (VO64_3867)	-	4358237..4358926 (+)	690	WP_046070859.1	energy-coupling factor ABC transporter permease	-
VO64_RS20470 (VO64_3868)	-	4358991..4359206 (+)	216	WP_003171680.1	hypothetical protein	-
VO64_RS20475 (VO64_3869)	yacG	4359203..4359403 (-)	201	WP_043049311.1	DNA gyrase inhibitor YacG	-
VO64_RS20480 (VO64_3870)	coaE	4359400..4360023 (-)	624	WP_046070860.1	dephospho-CoA kinase	-
VO64_RS20485 (VO64_3871)	pilD	4360020..4360886 (-)	867	WP_046070861.1	prepilin peptidase	Machinery gene
VO64_RS20490 (VO64_3872)	pilC	4360883..4362091 (-)	1209	WP_046070862.1	type II secretion system F family protein	Machinery gene
VO64_RS20495 (VO64_3873)	-	4362293..4362706 (+)	414	WP_046070863.1	pilin	-
VO64_RS20505 (VO64_3874)	-	4362993..4363298 (+)	306	WP_046070864.1	DUF6388 family protein	-
VO64_RS20510 (VO64_3875)	nadC	4363417..4364265 (-)	849	WP_046070865.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31816.23 Da
• Isoelectric Point: 8.3021

>NTDB_id=142502 VO64_RS20485 WP_046070861.1 4360020..4360886(-) (pilD) [Pseudomonas synxantha strain LBUM223]
MILLLTERPWFFVAMALVLGLIVGSFLNVLVWRLPKMLEREWRAQAREILGLPAAPGGPPYNLMCPNSCCTRCEQPIRPW
ENIPLLSYLLLRGRCAHCRERISLRYPLTELACALVSATVAWHFGLGWQAGAVLLLSWGLLGLSLIDLDHQLLPDALVLP
LLWLGLILNSADLLATLPDALWGAVIGYMSLWSVFWLFKLVTGKDGMGHGDFKLLALLGAWGGWQILPMTLLMASLLGLI
AGLILLRLRKTQASAPLPFGPCLAIAGWIALLWGGQITDFYLQSVGFR

Nucleotide

• Length: 867 bp

>NTDB_id=142502 VO64_RS20485 WP_046070861.1 4360020..4360886(-) (pilD) [Pseudomonas synxantha strain LBUM223]
TTGATCCTGCTGCTGACCGAACGGCCCTGGTTTTTTGTTGCCATGGCACTGGTGCTAGGTCTGATCGTCGGCAGTTTCCT
CAATGTGCTGGTGTGGCGACTGCCAAAAATGCTCGAGCGCGAATGGCGTGCCCAAGCCCGTGAGATCCTCGGCTTGCCTG
CTGCACCCGGCGGGCCGCCCTATAACCTGATGTGCCCGAATTCCTGCTGCACCCGTTGCGAACAGCCGATTCGGCCCTGG
GAAAATATCCCACTGCTCAGCTACCTGTTGCTCAGGGGGCGTTGTGCCCATTGCCGTGAGCGCATCAGCCTTCGTTATCC
CTTAACCGAGCTGGCCTGCGCGTTGGTCTCGGCCACCGTTGCCTGGCACTTCGGCCTTGGCTGGCAGGCCGGCGCGGTGC
TGCTGCTGAGCTGGGGCTTACTGGGCCTGAGCCTGATTGACCTCGACCATCAATTGTTGCCAGATGCGCTGGTGCTGCCG
CTGCTATGGCTGGGACTCATCCTCAACAGCGCCGACCTGCTGGCGACCTTGCCCGATGCGCTGTGGGGCGCGGTCATTGG
CTACATGAGCCTGTGGAGCGTGTTCTGGCTGTTCAAGCTGGTCACCGGTAAAGATGGCATGGGCCATGGCGACTTCAAGT
TATTGGCATTGCTGGGAGCCTGGGGTGGGTGGCAGATACTGCCGATGACATTACTGATGGCCTCATTGCTGGGCCTGATC
GCCGGGTTGATCCTGCTGCGTTTGCGCAAGACCCAAGCGTCAGCTCCGCTGCCCTTTGGTCCCTGCCTGGCAATTGCCGG
CTGGATTGCATTGCTCTGGGGTGGTCAAATAACCGACTTCTATTTGCAGTCTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-124)

• Type IV leader peptidase family (Peptidase_A24) (135-243)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.857	97.222	0.514
pilD	Vibrio campbellii strain DS40M4	50.534	97.569	0.493
pilD	Acinetobacter baumannii D1279779	48.718	94.792	0.462
pilD	Acinetobacter nosocomialis M2	48.718	94.792	0.462
pilD	Neisseria gonorrhoeae MS11	50	90.278	0.451