Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VO64_RS18500 Genome accession   NZ_CP011117
Coordinates   3914718..3916043 (+) Length   441 a.a.
NCBI ID   WP_046070558.1    Uniprot ID   -
Organism   Pseudomonas synxantha strain LBUM223     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3909718..3921043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VO64_RS18480 (VO64_3483) - 3909781..3912243 (-) 2463 WP_411741028.1 penicillin-binding protein 1A -
  VO64_RS18485 (VO64_3485) pilM 3912476..3913132 (+) 657 WP_046072635.1 type IV pilus assembly protein PilM -
  VO64_RS18490 (VO64_3486) - 3913129..3913680 (+) 552 WP_046070556.1 PilN domain-containing protein -
  VO64_RS18495 (VO64_3487) - 3913677..3914648 (+) 972 WP_046070557.1 pilus assembly protein PilP -
  VO64_RS18500 (VO64_3488) pilQ 3914718..3916043 (+) 1326 WP_046070558.1 type IV pilus secretin PilQ Machinery gene
  VO64_RS18505 (VO64_3489) aroK 3916048..3916566 (+) 519 WP_046070559.1 shikimate kinase AroK -
  VO64_RS18510 (VO64_3490) aroB 3916691..3917791 (+) 1101 WP_046070560.1 3-dehydroquinate synthase -
  VO64_RS18515 (VO64_3491) - 3917802..3919373 (+) 1572 WP_046070561.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47961.97 Da        Isoelectric Point: 5.2405

>NTDB_id=142501 VO64_RS18500 WP_046070558.1 3914718..3916043(+) (pilQ) [Pseudomonas synxantha strain LBUM223]
MAAAVPNRPDLIHLPPPGSALPVSYTGKKLTLNFQDIDLRAVLQQIADVAGLNLVTSDEVQGSITLRLKDVPWDQALDLV
LQTKGLDKRVKAGVLLVAPADELAARELLVLESRKQMAELAPLRRELLQVNYAKAADLAKLFQSMTGFEGATDERGSVAV
DDRTNNIIAYQTGERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGRLSRGNWGAGGISKPVADGAEPPQ
SPPSSPFVDLGSLTGTSGLGIAYITDNLLLDLELTAMEKSGNGEIVSQPKVVTSDKETARILKGTEIPYQESTSQGATSV
SFKEASLSLEVTPQITPDNHVFMAVKVTKDEPDYLNRLNDVPPIKKNEVNAKVLVRDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRNVLSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=142501 VO64_RS18500 WP_046070558.1 3914718..3916043(+) (pilQ) [Pseudomonas synxantha strain LBUM223]
ATGGCTGCCGCTGTGCCCAATCGCCCGGACCTGATCCACCTGCCGCCGCCTGGCAGTGCCTTGCCGGTCAGCTATACGGG
CAAAAAACTGACCCTCAATTTCCAGGACATCGACCTGCGCGCGGTATTGCAGCAAATCGCCGATGTCGCCGGGCTCAACC
TGGTGACCAGCGACGAGGTGCAGGGCTCGATCACCCTGCGGCTCAAGGACGTGCCCTGGGATCAGGCGCTGGACCTGGTG
CTGCAAACCAAGGGCCTGGACAAGCGGGTTAAGGCCGGCGTGTTGCTGGTGGCGCCCGCCGATGAATTGGCCGCTCGCGA
ACTGCTGGTGCTGGAGTCGCGCAAGCAGATGGCCGAGCTGGCACCCTTGCGTCGGGAATTATTGCAGGTCAATTACGCCA
AGGCTGCGGACCTGGCCAAGCTGTTCCAGTCGATGACCGGCTTTGAAGGTGCCACGGATGAACGCGGCTCGGTGGCGGTG
GATGACCGCACCAACAACATCATCGCCTACCAGACCGGCGAACGGCTGGAAGAGCTGCGGCGCATCGTGGCGCAGTTGGA
TATCCCGGTACGCCAGGTGATGATCGAGGCGCGAATCGTCGAGGCTAATGTCGACTACGACAAAAGCCTGGGCGTGCGCT
GGGGTGGGCGGCTCAGCCGCGGCAACTGGGGCGCCGGGGGTATTTCCAAGCCCGTGGCGGACGGCGCCGAGCCTCCGCAA
AGCCCGCCCAGCTCACCCTTCGTCGATCTGGGTTCGCTGACCGGCACGTCGGGGCTGGGTATCGCCTACATCACCGACAA
CCTATTGCTGGATCTTGAACTGACGGCGATGGAGAAAAGCGGCAACGGCGAAATCGTTTCGCAGCCCAAGGTGGTCACGT
CCGATAAAGAGACCGCACGCATCCTCAAGGGCACCGAGATTCCCTACCAGGAGTCCACCTCCCAGGGCGCTACCTCGGTG
TCGTTCAAGGAAGCGTCCCTTTCACTGGAGGTTACGCCGCAAATCACCCCCGATAACCACGTGTTCATGGCGGTCAAGGT
TACCAAGGACGAGCCTGACTATTTGAACCGGCTCAACGACGTGCCGCCGATCAAGAAAAACGAAGTCAACGCCAAGGTGC
TGGTCCGGGATGGCGAGACCATTGTCATCGGCGGGGTTTTCTCTAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTACCGTATCTTGGCCGCCTTTTCCGGCGCAATGTGCTGTCGGAGAAAAAATCTGAGCTGCTGGTATTCCT
GACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(54-99)

Bacterial type II and III secretion system protein (Secretin)

(276-432)

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.938

99.093

0.703
  comQ Acinetobacter baylyi ADP1

44.027

100

0.451
  pilQ Legionella pneumophila strain Lp02

45

95.238

0.429
  pilQ Acinetobacter baumannii strain A118

40.645

100

0.429
  pilQ Acinetobacter baumannii D1279779

40.645

100

0.429
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.721

97.506

0.426
  pilQ Vibrio cholerae strain A1552

43.721

97.506

0.426
  pilQ Vibrio campbellii strain DS40M4

44.104

96.145

0.424
  pilQ Legionella pneumophila strain ERS1305867

44.524

95.238

0.424

Multiple sequence alignment