Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PTUN_RS03500 Genome accession   NZ_CP011032
Coordinates   738301..739983 (-) Length   560 a.a.
NCBI ID   WP_009838313.1    Uniprot ID   A4C8Y7
Organism   Pseudoalteromonas tunicata strain D2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 733301..744983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PTUN_RS03475 (PTUN_a0826) - 733977..734600 (+) 624 WP_009838308.1 LysE family translocator -
  PTUN_RS03480 (PTUN_a0827) - 734597..735094 (+) 498 WP_009838309.1 gamma-glutamylcyclotransferase family protein -
  PTUN_RS03485 (PTUN_a0829) - 735290..736849 (+) 1560 WP_009838310.1 serine hydrolase -
  PTUN_RS03490 (PTUN_a0831) - 736960..737877 (+) 918 WP_009838311.1 histone deacetylase family protein -
  PTUN_RS03495 (PTUN_a0832) yacG 737991..738230 (-) 240 WP_009838312.1 DNA gyrase inhibitor YacG -
  PTUN_RS03500 (PTUN_a0833) pilB 738301..739983 (-) 1683 WP_009838313.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PTUN_RS03505 (PTUN_a0834) coaE 740131..740748 (-) 618 WP_009838314.1 dephospho-CoA kinase -
  PTUN_RS03510 (PTUN_a0836) pilD 740784..741689 (-) 906 WP_232284995.1 prepilin peptidase Machinery gene
  PTUN_RS03515 (PTUN_a0837) pilC 741709..742947 (-) 1239 WP_009838316.1 type II secretion system F family protein Machinery gene
  PTUN_RS03520 (PTUN_a0838) pilB 743044..744747 (-) 1704 WP_009838317.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 62316.41 Da        Isoelectric Point: 5.3462

>NTDB_id=141608 PTUN_RS03500 WP_009838313.1 738301..739983(-) (pilB) [Pseudoalteromonas tunicata strain D2]
MNLNSPLIRKFISLGYITPEDVSNKGLSSTSVIELITSSSDFTTKTFAAKCSELFKVPLFDLASFDPQHIPSELVNESLI
RKHHILPMSKRNRTLFLATADPTDFDAFENFEFNTGLQTEVVVVDFAELEKKIDQLLDNSSGLNLSDEEFKEFSDVEIDT
SEGTKDNDVADEKEDAPIIVYINKILMDAIRKGASDLHFEPYEFTYRIRFRIDGILHEVAAPPQALATRISARVKVMARL
DIAEKRKPQDGRIKLKITKKKSIDFRVSTLPTLWGEKIVMRILDSSSAMLGIDVLGYEVEQKELYLNALAQPQGMILVTG
PTGSGKTVSLYTGLNILNKPERNISTAEDPVEINLVGINQVQINARADMTFANALRAFLRQDPDVVMVGEIRDLETAEIS
IKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSVTLIIAQRLARRLCNNCKQPEELPEQELIRQGFSEQQSKEL
TLFKANGCELCTEGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAQKSKEQGFNNLRLSGLRKAAKGITSLAEINRVTSF

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=141608 PTUN_RS03500 WP_009838313.1 738301..739983(-) (pilB) [Pseudoalteromonas tunicata strain D2]
ATGAATTTAAACTCTCCCTTAATTCGAAAATTTATCAGCTTAGGGTACATAACACCTGAAGATGTAAGTAATAAGGGTTT
AAGCAGCACCTCGGTGATTGAGCTTATTACCAGCAGCTCAGATTTCACCACAAAAACATTTGCCGCCAAATGCAGTGAGT
TATTTAAAGTTCCTTTATTTGATTTAGCCAGCTTTGACCCGCAGCATATACCAAGCGAATTAGTAAACGAAAGTCTGATC
CGCAAACATCATATATTACCTATGAGCAAGCGTAACCGTACGCTGTTTTTAGCTACCGCCGACCCCACTGATTTTGATGC
TTTTGAAAATTTTGAATTTAATACTGGCTTACAAACCGAAGTGGTCGTAGTTGATTTTGCTGAGCTTGAGAAAAAGATTG
ATCAATTACTTGATAATTCATCTGGCTTAAATCTCAGTGATGAAGAGTTTAAAGAGTTTAGTGATGTTGAGATTGATACA
TCCGAAGGTACCAAAGACAACGATGTAGCCGATGAAAAAGAAGACGCTCCTATTATTGTTTATATCAATAAAATTTTAAT
GGATGCCATTCGTAAAGGAGCGTCTGATTTACACTTTGAACCCTATGAATTCACTTACCGAATTCGCTTTCGGATTGACG
GTATTTTGCATGAAGTAGCGGCCCCGCCACAAGCATTAGCAACTCGCATTTCGGCACGGGTTAAGGTTATGGCCCGTCTT
GATATTGCCGAAAAACGCAAACCACAAGATGGCCGCATTAAACTAAAAATCACTAAAAAGAAAAGTATCGATTTTCGTGT
TTCTACCTTGCCCACCCTTTGGGGTGAAAAAATCGTAATGCGGATTTTAGATTCATCAAGCGCCATGCTCGGCATTGATG
TATTAGGTTATGAAGTCGAACAAAAAGAGCTTTACTTAAATGCCTTAGCGCAGCCTCAAGGCATGATTTTAGTAACAGGT
CCAACAGGCTCAGGTAAAACCGTTTCACTTTATACCGGCTTAAATATTTTAAATAAACCAGAGCGCAATATTTCAACCGC
AGAAGATCCGGTTGAGATCAATTTAGTTGGCATTAATCAGGTGCAAATTAATGCGCGTGCCGATATGACCTTTGCCAATG
CTTTACGGGCATTTTTACGCCAAGATCCCGATGTCGTTATGGTTGGGGAAATTCGCGACCTCGAAACGGCTGAAATATCA
ATTAAAGCGGCGCAAACGGGCCATCTAGTGCTTTCAACCCTACATACGAACTCAGCGCCCGAAACGCTCACCCGCTTGCT
AAACATGGGCGTGCCAGCGTATAACGTCGCCAGTTCTGTAACCCTTATTATTGCCCAGCGTCTTGCTCGGCGCTTATGCA
ACAATTGCAAGCAACCAGAAGAGTTACCAGAACAAGAACTTATTCGTCAGGGCTTTTCTGAGCAGCAAAGTAAAGAACTC
ACCCTTTTTAAAGCAAATGGTTGCGAGCTTTGTACCGAAGGTTACAAAGGTCGGGTGGGTATTTACGAGGTAATGCAAAT
TACTCCAGAAATAGCACAAATTATTATGCGTGGCGGTAACTCGCTCGAAATTGCACAAAAGTCCAAAGAGCAAGGATTTA
ATAATTTACGCCTATCAGGCCTGCGCAAAGCCGCCAAAGGGATCACCAGTTTAGCCGAAATAAACCGCGTCACGAGTTTC
TAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-451)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A4C8Y7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.694

100

0.559
  pilB Acinetobacter baumannii D1279779

58.868

94.643

0.557
  pilB Legionella pneumophila strain ERS1305867

55.451

95

0.527
  pilB Vibrio parahaemolyticus RIMD 2210633

53.974

96.607

0.521
  pilB Vibrio cholerae strain A1552

52.661

97.321

0.513
  pilB Vibrio campbellii strain DS40M4

52.68

96.607

0.509
  pilF Neisseria gonorrhoeae MS11

47.681

96.25

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

36.613

100

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.189

100

0.373
  pilF Thermus thermophilus HB27

36.092

100

0.366
  pilB Haemophilus influenzae Rd KW20

38.697

93.214

0.361

Multiple sequence alignment