Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PTUN_RS03520 Genome accession   NZ_CP011032
Coordinates   743044..744747 (-) Length   567 a.a.
NCBI ID   WP_009838317.1    Uniprot ID   A4C8Z1
Organism   Pseudoalteromonas tunicata strain D2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 738044..749747
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PTUN_RS03500 (PTUN_a0833) pilB 738301..739983 (-) 1683 WP_009838313.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PTUN_RS03505 (PTUN_a0834) coaE 740131..740748 (-) 618 WP_009838314.1 dephospho-CoA kinase -
  PTUN_RS03510 (PTUN_a0836) pilD 740784..741689 (-) 906 WP_232284995.1 prepilin peptidase Machinery gene
  PTUN_RS03515 (PTUN_a0837) pilC 741709..742947 (-) 1239 WP_009838316.1 type II secretion system F family protein Machinery gene
  PTUN_RS03520 (PTUN_a0838) pilB 743044..744747 (-) 1704 WP_009838317.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PTUN_RS21925 - 745098..745203 (-) 106 Protein_684 pilin -
  PTUN_RS03530 (PTUN_a0841) nadC 745749..746639 (-) 891 WP_009838319.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PTUN_RS03535 (PTUN_a0842) ampD 746932..747477 (+) 546 WP_009838320.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PTUN_RS03540 (PTUN_a0843) ampE 747543..748385 (+) 843 WP_009838321.1 beta-lactamase regulator AmpE -
  PTUN_RS03545 (PTUN_a0844) pdhR 748831..749580 (+) 750 WP_009838322.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62879.36 Da        Isoelectric Point: 6.1613

>NTDB_id=141611 PTUN_RS03520 WP_009838317.1 743044..744747(-) (pilB) [Pseudoalteromonas tunicata strain D2]
MLLNSPLIRKLSNIGVIELESFRGINKTDKTSIPEIICKESSLTSIELAKKTSSLFQVPFIDLNSYDFQSLPNEKILNEK
LIKKHHVLPIAIKGKTIYLASADPTNFDAFEDYEFNTGMQTEVVVVNYEQLNLAIESLFDKQDGLELSDAQIAELEGVDG
EQETNSKKSLSTDVNNKDDDAPIIVYINKILMDAIKKGASDLHFEPYEHTYRIRFRVDGVLHEIANPPVGLESRISARIK
VMSHMDLAEKRKPQDGRIKLKITKKKSIDFRVSTLPTLWGEKIVMRILDSSSAMLGIDVLGYEPKQKDLYMNALAQPQGM
ILVTGPTGSGKTVSLYTGLNILNKPERNISTAEDPVEINLAGINQVQINIRADMTFANALRAFLRQDPDVVMVGEIRDLE
TAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPSYNIASSVSLIIAQRLARRLCSHCKEPEHIPTEELLKLGFTQT
DLTDLTLFKAKGCEHCTEGYKGRVGIYEVMPITPNIAQIIMRGGNSLEIAEAATNEGVNNLRRSGLIKAASGMTSIIEIN
RVTSYQP

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=141611 PTUN_RS03520 WP_009838317.1 743044..744747(-) (pilB) [Pseudoalteromonas tunicata strain D2]
ATGTTATTAAACTCCCCATTAATAAGAAAATTAAGTAACATTGGTGTTATCGAATTAGAAAGTTTTAGGGGGATAAATAA
GACTGATAAAACCTCCATTCCTGAAATAATTTGTAAAGAATCCAGCTTAACATCTATAGAATTAGCAAAAAAAACATCTT
CATTGTTTCAAGTTCCATTTATTGATTTGAACTCTTATGATTTCCAATCTCTCCCAAATGAGAAAATTTTAAATGAAAAG
TTAATTAAAAAACATCACGTTTTACCTATAGCAATAAAAGGCAAAACAATTTATCTCGCTAGCGCTGACCCTACCAACTT
TGATGCCTTTGAAGACTACGAATTCAATACCGGCATGCAAACCGAAGTGGTAGTGGTCAATTACGAGCAGCTTAACCTTG
CGATAGAGTCGTTATTTGATAAACAAGATGGCCTGGAACTTAGTGATGCGCAAATAGCAGAGCTAGAAGGCGTTGATGGC
GAGCAAGAGACTAATAGTAAAAAATCACTCTCTACCGATGTAAACAATAAAGATGACGATGCACCGATTATTGTTTATAT
CAATAAAATATTGATGGATGCGATTAAAAAAGGTGCATCTGACTTACATTTTGAACCCTATGAACATACTTATCGCATCC
GCTTTCGGGTTGATGGGGTATTACACGAAATAGCCAATCCGCCCGTCGGGCTTGAAAGCCGAATATCGGCTCGGATAAAA
GTCATGTCGCACATGGATTTGGCCGAAAAACGCAAACCACAAGATGGTCGTATTAAGCTAAAAATCACTAAAAAGAAAAG
CATCGACTTTCGTGTTTCGACCTTACCTACTTTGTGGGGCGAAAAGATTGTAATGCGTATTTTAGACTCATCAAGCGCTA
TGTTGGGCATTGATGTATTAGGTTACGAGCCAAAACAAAAAGATTTATATATGAATGCCCTTGCTCAGCCGCAAGGCATG
ATTTTAGTTACAGGCCCAACGGGTTCAGGTAAAACAGTATCGCTTTATACCGGTTTAAACATTCTGAATAAACCCGAGCG
TAATATTTCAACCGCAGAAGACCCGGTTGAAATTAACTTAGCCGGTATCAACCAAGTACAAATAAATATTCGTGCTGATA
TGACCTTTGCTAATGCCCTGCGCGCGTTTTTACGCCAAGATCCTGATGTCGTCATGGTGGGGGAGATTCGTGATCTCGAA
ACCGCCGAAATCGCCATCAAAGCCGCGCAAACGGGTCACTTAGTACTTTCTACTTTGCATACCAACTCAGCGCCAGAGAC
CCTTACTCGTTTGATGAACATGGGTGTGCCGTCTTATAATATCGCTAGTTCAGTGAGTTTAATTATAGCCCAGCGCCTAG
CGCGTCGTTTATGCAGCCACTGTAAAGAGCCTGAGCATATTCCTACTGAAGAGTTATTAAAGCTTGGCTTTACCCAAACT
GACTTAACCGATTTAACCTTATTTAAAGCCAAGGGCTGTGAGCACTGTACAGAAGGCTATAAAGGCCGCGTTGGTATTTA
TGAAGTGATGCCCATTACCCCTAATATTGCGCAAATTATTATGCGCGGTGGTAACTCCCTTGAAATAGCAGAAGCGGCAA
CTAACGAAGGGGTGAATAATCTTCGCCGTTCTGGCTTAATAAAGGCGGCATCGGGTATGACGTCGATTATTGAAATAAAT
CGCGTGACTAGCTATCAACCATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-143)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A4C8Z1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.3

99.824

0.552
  pilB Acinetobacter baumannii D1279779

58.302

93.474

0.545
  pilB Vibrio campbellii strain DS40M4

52.007

100

0.526
  pilB Vibrio parahaemolyticus RIMD 2210633

54.197

96.649

0.524
  pilB Legionella pneumophila strain ERS1305867

54.144

95.767

0.519
  pilB Vibrio cholerae strain A1552

53.42

95.414

0.51
  pilF Neisseria gonorrhoeae MS11

47.834

97.707

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

36.774

100

0.402
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.547

94.709

0.365

Multiple sequence alignment