Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RY45_RS20050 Genome accession   NZ_CP010947
Coordinates   4410006..4411712 (+) Length   568 a.a.
NCBI ID   WP_044800923.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain AL06-06 isolate Goldfish     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4405006..4416712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RY45_RS20025 (RY45_20010) pdhR 4406158..4406922 (-) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  RY45_RS23345 ampD 4407198..4407770 (-) 573 WP_080891465.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RY45_RS20035 (RY45_20020) - 4407918..4408388 (+) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  RY45_RS20040 (RY45_20025) nadC 4408394..4409251 (+) 858 WP_044800921.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RY45_RS23355 (RY45_20030) tapA 4409574..4410002 (+) 429 WP_044800922.1 type IVa pilus major pilin TapA -
  RY45_RS20050 (RY45_20035) pilB 4410006..4411712 (+) 1707 WP_044800923.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  RY45_RS20055 (RY45_20040) pilC 4411828..4413069 (+) 1242 WP_017409439.1 type II secretion system F family protein Machinery gene
  RY45_RS20060 (RY45_20045) pilD 4413092..4413964 (+) 873 WP_044800924.1 A24 family peptidase Machinery gene
  RY45_RS20065 (RY45_20050) coaE 4413984..4414598 (+) 615 WP_044800925.1 dephospho-CoA kinase -
  RY45_RS20070 (RY45_20055) zapD 4414636..4415358 (+) 723 WP_024945431.1 cell division protein ZapD -
  RY45_RS20075 (RY45_20060) yacG 4415368..4415562 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  RY45_RS20080 (RY45_20065) mutT 4415623..4416036 (-) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62509.87 Da        Isoelectric Point: 5.4342

>NTDB_id=140835 RY45_RS20050 WP_044800923.1 4410006..4411712(+) (pilB) [Aeromonas hydrophila strain AL06-06 isolate Goldfish]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAISKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVLVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=140835 RY45_RS20050 WP_044800923.1 4410006..4411712(+) (pilB) [Aeromonas hydrophila strain AL06-06 isolate Goldfish]
ATGACCTCTAGCCCCAATAGCGGCCTGGCATTAAGCCTGGCCGCCTCCTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCACAGCGCAAACCTTTTGTGACCTTTCTGATCGAAAACGAAATCCTCGACAGCAAGGCACTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAACATCACGTCCTGCCCATCTACACCCAGGGACACACCCTCTATATTGCCATGTCGGA
TCCCACCAATGTGTCTGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTAGTCGAGGAGAACA
AACTCACCACAGCCATCAGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAACTGGAGGTCTCGGATGAGGGCTCGCGGCTCGACGAAAGCGTCAATACGGCCGACGATGACGCCCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGTGGGGCCTCCGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGTTTTCGGATAGACGGGATCTTGCATGAAGTAGCGACTCCACCTGTCAATCTGGCCAACCGCTTCTCGGCCCGC
CTCAAGGTAATGGCTCGGCTCGACATCGCCGAGCGGCGACTGCCCCAAGATGGTCGCATCAAACTGAAGCTGTCGCGCAA
CAAGTCGATGGACATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCCTCCG
CGGCACGCCTCAATATCGAGCAGCTCGGGTTTGACGATCGCCAGAAGGCGCAATATCTGCGAGCCCTCTCGAAGCCGCAA
GGCATGATCCTGGTGACAGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGCCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAATCCCAAGG
CAGGACTGACCTTCGCGAGCGCCCTGCGCTCTTTCTTGCGCCAAGACCCCGACGTGGTCTTGGTGGGTGAAATCCGGGAT
CTGGAAACAGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACTTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
GGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGTAAATTGTGCGATCACTGCAAAGCTCCCGAAGTGGTACCTGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCAGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAGGAGTGTTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGTACCTTGCGTACCTCCGGCCTTGAGAAGGCCCGCCTAGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Acinetobacter baumannii D1279779

55.185

100

0.553
  pilB Legionella pneumophila strain ERS1305867

54.336

99.472

0.54
  pilB Vibrio cholerae strain A1552

53.982

99.472

0.537
  pilB Vibrio campbellii strain DS40M4

51.045

100

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

51.246

98.944

0.507
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

39.962

92.958

0.371

Multiple sequence alignment