Detailed information NTG0140810

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: B723_RS19345
• Genome accession: NZ_CP010945
• Coordinates: 4205976..4207085 (+)
• Length: 369 a.a.
• NCBI ID: WP_031318970.1
• Uniprot ID: A0A0K1QZI0
• Organism: Pseudomonas fluorescens NCIMB 11764
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4200976..4212085

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B723_RS19330 (B723_19365)	-	4201848..4202783 (+)	936	WP_017339147.1	sugar kinase	-
B723_RS19335 (B723_19370)	-	4202947..4203222 (-)	276	WP_007898282.1	peptidylprolyl isomerase	-
B723_RS19340 (B723_19375)	-	4203323..4205857 (-)	2535	WP_017339146.1	PAS domain-containing protein	-
B723_RS19345 (B723_19380)	pilU	4205976..4207085 (+)	1110	WP_031318970.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
B723_RS19350 (B723_19385)	-	4207114..4209069 (-)	1956	WP_017339144.1	acetoacetate--CoA ligase	-
B723_RS19355 (B723_19390)	hbdH	4209231..4210004 (-)	774	WP_017339143.1	3-hydroxybutyrate dehydrogenase	-
B723_RS19360 (B723_19395)	-	4210012..4211403 (-)	1392	WP_017339142.1	GntP family permease	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41326.31 Da
• Isoelectric Point: 6.1535

>NTDB_id=140810 B723_RS19345 WP_031318970.1 4205976..4207085(+) (pilU) [Pseudomonas fluorescens NCIMB 11764]
MEIDGLLKILASQEGSDLYLSTGAPPCARFDGVLKPVTDQPFKPGEITAIAMSIMDAEQRLEFDRELEMNLAISLPGVGR
FRVNIFKQRNDMSIVVRNVKLDIPRFEDLRLPAVLLETVMLKQGLMLFVGATDSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNAHQALDRI
INFFPEERRTQLLHDLGNNLKAFVSQRLVRTRDGQRRAAVEVMLGSPTIGDLIRRNEFDELKGIMEKSVEQGMQTFDSAL
FALVEEGAIDEEEALKHADSVNNLRLRLKLHAETSPGPHAPPGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=140810 B723_RS19345 WP_031318970.1 4205976..4207085(+) (pilU) [Pseudomonas fluorescens NCIMB 11764]
ATGGAAATCGATGGGCTTTTGAAGATTCTGGCCAGCCAGGAGGGTTCCGATCTGTACCTGTCCACCGGCGCGCCACCCTG
CGCACGGTTTGATGGTGTGCTTAAACCCGTGACCGACCAGCCATTCAAACCGGGGGAAATCACGGCGATTGCCATGTCCA
TCATGGACGCCGAGCAGCGGCTGGAGTTCGATCGGGAACTGGAGATGAACCTGGCGATCTCTTTGCCGGGTGTCGGGCGT
TTTCGGGTCAACATTTTCAAGCAGCGCAACGACATGTCGATTGTGGTGCGTAATGTCAAACTCGACATTCCACGTTTCGA
AGACCTCCGGTTGCCAGCGGTCCTGCTGGAAACGGTCATGCTCAAACAGGGGCTGATGCTGTTCGTCGGCGCCACCGACT
CAGGCAAGTCTACCTCACTGGCGGCGTTGATCGATTATCGCAACCGCCACAGCAGCGGCCATATCATCACCATCGAAGAC
CCGGTCGAATACATTCATCGGCACAAGAAGTCGATCATCAATCAGCGCGAGGTCGGCGTGGATACCCGCAGTTTTCATGC
GGCGTTGAAGAATACCCTGCGCCAGGCACCGGATGTGGTGCTGATCGGCGAGATCCGCGACCGCGAAACCATGGAGCATG
CGTTGGCGTTTGCCGACACCGGGCACCTGGTGATTTCCACCTTGCATGCCCACAACGCCCATCAGGCGCTGGACCGTATC
ATCAACTTCTTCCCGGAGGAGCGGCGGACGCAACTGCTGCATGACCTGGGCAACAATCTCAAAGCGTTCGTTTCCCAACG
GCTTGTGCGTACCCGTGATGGGCAACGGAGGGCAGCGGTGGAAGTGATGCTCGGTTCGCCGACCATTGGTGACTTGATCC
GCCGTAACGAATTCGATGAGCTCAAGGGGATCATGGAGAAGTCCGTCGAGCAGGGCATGCAGACGTTCGATAGCGCGCTG
TTCGCGTTGGTTGAAGAGGGTGCGATCGATGAGGAGGAGGCGCTGAAGCACGCGGACTCGGTGAACAATTTGCGCCTGCG
ACTGAAGCTGCATGCGGAAACATCACCGGGCCCCCATGCACCACCCGGCGAATGGGGACTGATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-271)

3D structure

• AlphaFold DB: A0A0K1QZI0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.62	99.729	0.545
pilU	Acinetobacter baylyi ADP1	51.541	96.748	0.499
pilU	Vibrio cholerae strain A1552	50.571	94.851	0.48
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	37.5	99.729	0.374
pilT	Pseudomonas stutzeri DSM 10701	39.826	93.225	0.371
pilT	Pseudomonas aeruginosa PAK	39.826	93.225	0.371
pilT	Vibrio cholerae strain A1552	40.541	90.244	0.366
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.541	90.244	0.366